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Separation of protein mixtures by an integrated electro-ultrafiltration-electrodialysis process
Chen, Guoqiang1,2; Song, Weijie1; Qi, Benkun1; Li, Jing1; Ghosh, Raja3; Wan, Yinhua1
2015-06-16
Source PublicationSEPARATION AND PURIFICATION TECHNOLOGY
ISSN1383-5866
Volume147Issue:JUNPages:32-43
Abstract

An integrated process for improving selectivity and permeate flux in ultrafiltration based protein fractionation was developed by combining electrodialysis (ED) with electro-ultrafiltration (EUF). The performance of such a process was first investigated using individual proteins (bovine serum albumin and lysozyme) and then with a mixture of the two. The experimental results showed that as with EUF on its own, the build-up of lysozyme concentration polarization near the cation exchange membrane in the permeate compartment significantly affected ion migration and led to change in pH and conductivity of the feed solution during an EUF-ED process with lysozyme, or bovine serum albumin and lysozyme mixture. As compared with EUF, EUF-ED of protein mixture resulted in a 20% increase in permeate flux with the lysozyme transmission remaining the same. The demineralization that occurred during EUF-ED made this process suitable for protein separation from the feed solutions with high conductivity. (C) 2015 Elsevier B.V. All rights reserved.

KeywordSeparation Ultrafiltration Electro-ultrafiltration Electrodialysis Protein
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1016/j.seppur.2015.04.003
Indexed BySCI
Language英语
WOS KeywordBIOACTIVE PEPTIDES ; MEMBRANE ; ELECTROULTRAFILTRATION ; REMOVAL ; WATER ; FRACTIONATION ; DESALINATION ; HYDROLYSATE ; JUICE
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
WOS IDWOS:000356553300005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19298
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.McMaster Univ, Dept Chem Engn, Hamilton, ON L8S 4L7, Canada
Recommended Citation
GB/T 7714
Chen, Guoqiang,Song, Weijie,Qi, Benkun,et al. Separation of protein mixtures by an integrated electro-ultrafiltration-electrodialysis process[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2015,147(JUN):32-43.
APA Chen, Guoqiang,Song, Weijie,Qi, Benkun,Li, Jing,Ghosh, Raja,&Wan, Yinhua.(2015).Separation of protein mixtures by an integrated electro-ultrafiltration-electrodialysis process.SEPARATION AND PURIFICATION TECHNOLOGY,147(JUN),32-43.
MLA Chen, Guoqiang,et al."Separation of protein mixtures by an integrated electro-ultrafiltration-electrodialysis process".SEPARATION AND PURIFICATION TECHNOLOGY 147.JUN(2015):32-43.
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