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Purification and characterization of a novel glycoprotein from Streptomyces sp ZX01
Zhang, Guoqiang1; Han, Lirong1; Zhang, Guifeng2; Zhang, Xing1; Feng, Juntao1
2015-07-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume78Issue:JULPages:195-201
Abstract

A novel glycoprotein GP-1 with antiviral activity against plant virus was isolated from the fermentation broth of the actinomycete Streptomyces sp. ZX01. MALDI-TOF-MS proved that molecular weight of GP-1 approximately was 8.5 kDa. GP-1 was a heat-sensitive glycoprotein with decreasing antiviral activity after treated from 80 degrees C to 100 degrees C for 30 min. GP-1 contained 40.23% carbohydrate with N-linked and O-linked glycan. FT-IR and NMR spectra proved that GP-1 contained protein and carbohydrate portions with alpha-D-(1,6)-glucose residues. Circular dichroism revealed that GP-1 was a glycoprotein with a large unordered content. Moreover, protein sequencing was predicted by using MALDI-TOF-MS and Mascot search. These results suggested that glycoprotein GP-1 could be used as a novel natural antiviral agent in agricultural industry. (C) 2015 Elsevier B.V. All rights reserved.

KeywordStreptomyces Sp Zx01 Antiviral Activity Tobacco Mosaic Virus
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.ijbiomac.2015.04.012
Indexed BySCI
Language英语
WOS KeywordMYCOBACTERIUM-TUBERCULOSIS ; LIVIDANS 66 ; STRUCTURAL ELUCIDATION ; CULTURED MYCELIA ; BINDING-PROTEIN ; GLYCOSYLATION ; HYDROXYPROLINE ; BIOSYNTHESIS ; IMPROVEMENT ; PATHOGENS
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000356195400026
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19308
Collection研究所(批量导入)
Affiliation1.Northwest A&F Univ, Res & Dev Ctr Biorat Pesticide, Yangling 712100, Shaanxi, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Guoqiang,Han, Lirong,Zhang, Guifeng,et al. Purification and characterization of a novel glycoprotein from Streptomyces sp ZX01[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,78(JUL):195-201.
APA Zhang, Guoqiang,Han, Lirong,Zhang, Guifeng,Zhang, Xing,&Feng, Juntao.(2015).Purification and characterization of a novel glycoprotein from Streptomyces sp ZX01.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,78(JUL),195-201.
MLA Zhang, Guoqiang,et al."Purification and characterization of a novel glycoprotein from Streptomyces sp ZX01".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 78.JUL(2015):195-201.
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