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Stabilization of Phenylalanine Ammonia Lyase from Rhodotorula glutinis by Encapsulation in Polyethyleneimine-Mediated Biomimetic Silica
Cui, Jiandong1,2,3; Liang, Longhao1; Han, Cong1; Liu, Rong Lin1
2015-07-01
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
Volume176Issue:4Pages:999-1011
Abstract

Phenylalanine ammonia lyase (PAL) from Rhodotorula glutinis was encapsulated within polyethyleneimine-mediated biomimetic silica. The main factors in the preparation of biomimetic silica were optimized by response surface methodology (RSM). Compared to free PAL (about 2 U), the encapsulated PAL retained more than 43 % of their initial activity after 1 h of incubation time at 60 degrees C, whereas free PAL lost most of activity in the same conditions. It was clearly indicated that the thermal stability of PAL was improved by encapsulation. Moreover, the encapsulated PAL exhibited the excellent stability of the enzyme against denaturants and storage stability, and pH stability was improved by encapsulation. Operational stability of 7 reaction cycles showed that the encapsulated PAL was stable. Nevertheless, the K-m value of encapsulated PAL in biomimetic silica was higher than that of the free PAL due to lower total surface area and increased mass transfer resistance.

KeywordPhenylalanine Ammonia Lyase Biomimetic Silica Immobilization Enzyme Rhodotorula Glutinis Stability
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s12010-015-1624-0
Indexed BySCI
Language英语
WOS KeywordENZYME IMMOBILIZATION ; ORGANIC-SOLVENTS ; PHENYLKETONURIA ; PERFORMANCE ; IMPROVE ; COAGGREGATION ; BIOCATALYSTS ; AMINATION ; CLEAS ; COLI
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000358379900004
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19408
Collection生化工程国家重点实验室
Affiliation1.Hebei Univ Sci & Technol, Coll Biosci & Bioengn, Res Ctr Fermentat Engn Hebei, Shijiazhuang 050018, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Tianjin Univ Sci & Technol, Key Lab Ind Microbiol, Minist Educ, Tianjin 300457, Peoples R China
Recommended Citation
GB/T 7714
Cui, Jiandong,Liang, Longhao,Han, Cong,et al. Stabilization of Phenylalanine Ammonia Lyase from Rhodotorula glutinis by Encapsulation in Polyethyleneimine-Mediated Biomimetic Silica[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2015,176(4):999-1011.
APA Cui, Jiandong,Liang, Longhao,Han, Cong,&Liu, Rong Lin.(2015).Stabilization of Phenylalanine Ammonia Lyase from Rhodotorula glutinis by Encapsulation in Polyethyleneimine-Mediated Biomimetic Silica.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,176(4),999-1011.
MLA Cui, Jiandong,et al."Stabilization of Phenylalanine Ammonia Lyase from Rhodotorula glutinis by Encapsulation in Polyethyleneimine-Mediated Biomimetic Silica".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 176.4(2015):999-1011.
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