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Development of novel small peptide ligands for antibody purification
Wei, Yuping1,2; Xu, Jiandong1; Zhang, Liang1,2; Fu, Yankai1,2; Xu, Xia1
2015
Source PublicationRSC ADVANCES
ISSN2046-2069
Volume5Issue:82Pages:67093-67101
Abstract

The huge demand for the most promising biopharmaceuticals, monoclonal antibodies (mAb), has resulted in a need for more efficient and less costly downstream purification processes for mAb. The currently used Protein A Agarose is unattractive due to toxic ligand leakage and high cost. In this study, three novel small peptide ligands, DWHW, CEWW and HEYW, were designed based on the interactions with human immunoglobulin G (IgG) using molecular simulations. The effects of pH, ionic strength and flow rate on the binding capacities to IgG were investigated. The static and dynamic binding capacities were determined. The dissociation constant (K-d) of the DWHW resin was 1.1 x 10(-5) M. The binding capacity of the DWHW resin was 24.5 mg ml(-1), which is comparable to Protein A Agarose. The DWHW resin was able to purify IgG from cMEM and CHO cell culture supernatants with a purity of more than 95% under elution conditions of pH 9 (50 mM sodium borate buffer) and pH 3 (50 mM glycine-HCl buffer). The results indicate that the small peptide ligands, especially DWHW, can offer a potential alternative for mAb purification.

SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1039/c5ra07829f
Indexed BySCI
Language英语
WOS KeywordAFFINITY-CHROMATOGRAPHY ; PROTEIN-A ; MONOCLONAL-ANTIBODIES ; BIOMIMETIC LIGANDS ; BINDING-SITE ; IGG ; IMMUNOGLOBULINS ; INDUCTION ; CAPACITY ; DOCKING
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000359251600069
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19449
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wei, Yuping,Xu, Jiandong,Zhang, Liang,et al. Development of novel small peptide ligands for antibody purification[J]. RSC ADVANCES,2015,5(82):67093-67101.
APA Wei, Yuping,Xu, Jiandong,Zhang, Liang,Fu, Yankai,&Xu, Xia.(2015).Development of novel small peptide ligands for antibody purification.RSC ADVANCES,5(82),67093-67101.
MLA Wei, Yuping,et al."Development of novel small peptide ligands for antibody purification".RSC ADVANCES 5.82(2015):67093-67101.
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