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Aggregation and antigenicity of virus like particle in salt solution-A case study with hepatitis B surface antigen
Chen, Yi1,2; Zhang, Yan1; Quan, Can3; Luo, Jian1; Yang, Yanli1; Yu, Mengran1; Kong, Yingjun1; Ma, Guanghui1; Su, Zhiguo1
2015-08-20
Source PublicationVACCINE
ISSN0264-410X
Volume33Issue:35Pages:4300-4306
AbstractThe phenomenon of aggregation of virus-like particles (VLPs) in salt solution and the corresponding effect upon antigenicity was reported. Asymmetrical flow field-flow fractionation (AF4) combined with multi-angle laser light scattering (MALLS) was used to characterize the size and the aggregation behavior of hepatitis 13 surface antigen (HBsAg). The average diameter of HBsAg VLP was 22.8 +/- 0.4 nm and it tended to aggregate in salt solution to form large particles and the antigenicity changed accordingly. In 0-4 M NaCl solution, part of HBsAg molecules aggregated rapidly into oligomeric particles (OP), whose diameter distributed from 25 to 40 nm, and the antigenicity slightly decreased about 10%. The aggregation reaction is reversible. After removing NaCl, both size and antigenicity could recover to normal level (92-96%). By contrast, the aggregation process is more complicated in (NH4)(2)SO4 solution. Most of HBsAg particles aggregated into OP and further aggregated into polymeric particles (PP). The diameter of the PP could reach 40 to 140 nm. The concentration of (NH4)(2)SO4 had remarkable influence upon the rate of aggregation. When concentration of (NH4)(2)SO4 was below 1 M, most of HBsAg aggregated only into OP in 1 h. While with concentration of (NH4)(2)SO4 above 1 M, most of particles formed PP within 1 h. The aggregation process to PP was irreversible. After removing (NH4)(2)SO4, the large aggregates could not recover to normal particles and the remaining antigenicity was below 30%. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordAsymmetrical Flow field-Flow Fractionation Virus-like Particle Aggregation Antigenicity Hepatitis b Surface Antigen
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.vaccine.2015.03.078
Indexed BySCI
Language英语
WOS KeywordFIELD-FLOW FRACTIONATION ; SIZE-EXCLUSION CHROMATOGRAPHY ; LASER-LIGHT SCATTERING ; QUANTITATIVE CHARACTERIZATION ; ELECTRON-MICROSCOPY ; VACCINE FORMULATION ; STABILIZATION ; DELIVERY ; PROTEIN
WOS Research AreaImmunology ; Research & Experimental Medicine
WOS SubjectImmunology ; Medicine, Research & Experimental
WOS IDWOS:000360870500012
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Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19538
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Natl Inst Metrol, Div Chem, Beijing 100013, Peoples R China
Recommended Citation
GB/T 7714
Chen, Yi,Zhang, Yan,Quan, Can,et al. Aggregation and antigenicity of virus like particle in salt solution-A case study with hepatitis B surface antigen[J]. VACCINE,2015,33(35):4300-4306.
APA Chen, Yi.,Zhang, Yan.,Quan, Can.,Luo, Jian.,Yang, Yanli.,...&Su, Zhiguo.(2015).Aggregation and antigenicity of virus like particle in salt solution-A case study with hepatitis B surface antigen.VACCINE,33(35),4300-4306.
MLA Chen, Yi,et al."Aggregation and antigenicity of virus like particle in salt solution-A case study with hepatitis B surface antigen".VACCINE 33.35(2015):4300-4306.
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