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Activation of glycerol metabolic pathway by evolutionary engineering of Rhizopus oryzae to strengthen the fumaric acid biosynthesis from crude glycerol
Huang, Di1,2,3; Wang, Ru1,2; Du, Wenjie4; Wang, Guanyi4; Xia, Menglei5,6
2015-11-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume196Issue:NOVPages:263-272
AbstractRhizopus oryzae is strictly inhibited by biodiesel-based by-product crude glycerol, which results in low fumaric acid production. In this study, evolutionary engineering was employed to activate the glycerol utilization pathway for fumaric acid production. An evolved strain G80 was selected, which could tolerate and utilize high concentrations of crude glycerol to produce 14.9 g/L fumaric acid with a yield of 0.248 g/g glycerol. Key enzymes activity analysis revealed that the evolved strain displayed a significant upregulation in glycerol dissimilation, pyruvate consumption and reductive tricarboxylic acid pathways, compared with the parent strain. Subsequently, intracellular metabolic profiling analysis showed that amino acid biosynthesis, tricarboxylic acid cycle, fatty acid and stress response metabolites accounted for metabolic difference between two strains. Moreover, a glycerol fed-batch strategy was optimized to obtain the highest fumaric acid production of 25.5 g/L, significantly increased by 20.9-fold than that of the parent strain of 1.2 g/L. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordCrude Glycerol Evolutionary Engineering Intracellular Metabolic Profiling Fumaric Acid Rhizopus Oryzae
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2015.07.104
Indexed BySCI
Language英语
WOS KeywordESCHERICHIA-COLI ; SACCHAROMYCES-CEREVISIAE ; LACTATE-DEHYDROGENASE ; KLEBSIELLA-PNEUMONIAE ; PROFILING ANALYSIS ; FERMENTATION ; 1,3-PROPANEDIOL ; GLUCOSE ; STRAIN ; XYLOSE
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000361040500035
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19541
Collection生化工程国家重点实验室
Affiliation1.Nankai Univ, TEDA, TEDA Inst Biol Sci & Biotechnol, Tianjin 300457, Peoples R China
2.Minist Educ, Key Lab Mol Microbiol & Technol, Tianjin 300071, Peoples R China
3.Tianjin Key Lab Microbial Funct Genom, Tianjin 300457, Peoples R China
4.Tianjin Univ, Key Lab Syst Bioengn, Minist Educ, Tianjin 300072, Peoples R China
5.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
6.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Huang, Di,Wang, Ru,Du, Wenjie,et al. Activation of glycerol metabolic pathway by evolutionary engineering of Rhizopus oryzae to strengthen the fumaric acid biosynthesis from crude glycerol[J]. BIORESOURCE TECHNOLOGY,2015,196(NOV):263-272.
APA Huang, Di,Wang, Ru,Du, Wenjie,Wang, Guanyi,&Xia, Menglei.(2015).Activation of glycerol metabolic pathway by evolutionary engineering of Rhizopus oryzae to strengthen the fumaric acid biosynthesis from crude glycerol.BIORESOURCE TECHNOLOGY,196(NOV),263-272.
MLA Huang, Di,et al."Activation of glycerol metabolic pathway by evolutionary engineering of Rhizopus oryzae to strengthen the fumaric acid biosynthesis from crude glycerol".BIORESOURCE TECHNOLOGY 196.NOV(2015):263-272.
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