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Fine-tuning of ecaA and pepc gene expression increases succinic acid production in Escherichia coli
Wang, Jing1; Qin, Dandan1; Zhang, Baoyun2; Li, Qiang3; Li, Sha4; Zhou, Xiaohua1; Dong, Lichun1; Wang, Dan1,4
2015-10-01
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
Volume99Issue:20Pages:8575-8586
Abstract

For making a complex synthetic gene network function as designed, the parameters in the network have to be extensively tuned. In this study, a simple and general approach to rapidly tune gene networks in Escherichia coli was developed, which uses the hypermutable simple sequence repeats embedded in the spacer region between the ribosome binding site and the initiation codon. It was found that the change of sequence length and compositions of the repeated base pairs in 5'UTR contributes together to the changeable expression levels of the target gene. The mechanism of this phenomenon is that the transcriptional process makes greater impact on the expression level when compared to the translational process, which is utilized to successfully predict sample gene expression levels over a 50-fold range. The utility of the approach to regulate heterologous ecaA and pepc gene expression in the engineered E. coli for improving succinic acid yield and production has also been demonstrated. When the expression level of ecaA gene was 3.53-fold of the control and the expression level of pepc gene was 1.06-fold of the control, the highest yield of succinic acid and productivity were achieved, which was 0.87 g g(-1) and 2.01 g L-1 h(-1), respectively.

KeywordGene Regulation Rna Polymerase Holoenzyme Rbs Transcriptional Level Translational Level Succinic Acid
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00253-015-6734-7
Indexed BySCI
Language英语
WOS KeywordRIBOSOME BINDING-SITES ; SYNTHETIC BIOLOGY ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; 2,3-BUTANEDIOL PRODUCTION ; MESSENGER-RNAS ; CO2 ; OVEREXPRESSION ; DEHYDROGENASE ; TRANSLATION ; CHEMICALS
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000361493200022
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19571
Collection生化工程国家重点实验室
Affiliation1.Chongqing Univ, Sch Chem & Chem Engn, Chongqing 400044, Peoples R China
2.Chongqing Univ, Sch Bioengn, Chongqing 400044, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
4.Nanjing Univ Technol, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Wang, Jing,Qin, Dandan,Zhang, Baoyun,et al. Fine-tuning of ecaA and pepc gene expression increases succinic acid production in Escherichia coli[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2015,99(20):8575-8586.
APA Wang, Jing.,Qin, Dandan.,Zhang, Baoyun.,Li, Qiang.,Li, Sha.,...&Wang, Dan.(2015).Fine-tuning of ecaA and pepc gene expression increases succinic acid production in Escherichia coli.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,99(20),8575-8586.
MLA Wang, Jing,et al."Fine-tuning of ecaA and pepc gene expression increases succinic acid production in Escherichia coli".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 99.20(2015):8575-8586.
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