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Utilization of alkaline phosphatase PhoA in the bioproduction of geraniol by metabolically engineered Escherichia coli
Liu, Wei1,2,3; Zhang, Rubing1,3; Tian, Ning1; Xu, Xin1; Cao, Yujing1; Xian, Mo1; Liu, Huizhou1
2015-09-01
Source PublicationBIOENGINEERED
ISSN2165-5979
Volume6Issue:5Pages:288-293
AbstractGeraniol is a valuable acyclic monoterpene alcohol and has many applications in the perfume industries, pharmacy and others. It has been hypothesized that phosphatases can convert geranyl diphosphate (GPP) into geraniol. However, whether and which phosphatases can transform GPP to geraniol has remained unanswered up till now. In this paper, the catalysis abilities of 4 different types of phosphatases were studied with GPP as substrate in vitro. They are bifunctional diacylglycerol diphosphate phosphatase (DPP1) and lipid phosphate phosphatase (LPP1) from Saccharomyces cerevisiae, ADP-ribose pyrophosphatase (NudF) and alkaline phosphatase (PhoA) from Escherichia coli. The results show that just PhoA from E. coli can convert GPP into geraniol. Moreover, in order to confirm the ability of PhoA in vivo, the heterologous mevalonate pathway and geranyl diphosphate synthase gene from Abies grandis were co-overexpressed in E. coli with PhoA gene and 5.3 +/- 0.2mg/l geraniol was produced from glucose in flask-culture. Finally, we also evaluated the fed-batch fermentation of this engineered E. coli and a maximum concentration of 99.3mg/l geraniol was produced while the conversion efficiency of glucose to geranoid (gram to gram) was 0.51%. Our results offer a new option for geraniol biosynthesis and promote the industrial bio-production of geraniol.
KeywordBiosynthesis Engineered Escherichia Coli Geraniol Phoa Phosphatase
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1080/21655979.2015.1062188
Indexed BySCI
Language英语
WOS KeywordSACCHAROMYCES-CEREVISIAE ; SWEET BASIL ; BIOSYNTHESIS ; SYNTHASE ; PATHWAY ; GLANDS
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000362787000005
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19609
Collection湿法冶金清洁生产技术国家工程实验室
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liu, Wei,Zhang, Rubing,Tian, Ning,et al. Utilization of alkaline phosphatase PhoA in the bioproduction of geraniol by metabolically engineered Escherichia coli[J]. BIOENGINEERED,2015,6(5):288-293.
APA Liu, Wei.,Zhang, Rubing.,Tian, Ning.,Xu, Xin.,Cao, Yujing.,...&Liu, Huizhou.(2015).Utilization of alkaline phosphatase PhoA in the bioproduction of geraniol by metabolically engineered Escherichia coli.BIOENGINEERED,6(5),288-293.
MLA Liu, Wei,et al."Utilization of alkaline phosphatase PhoA in the bioproduction of geraniol by metabolically engineered Escherichia coli".BIOENGINEERED 6.5(2015):288-293.
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