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Fabrication of high-capacity protein ion-exchangers with polymeric ion-exchange groups grafted onto micron-sized beads by atom transfer radical polymerization
Li, Shu1,2,3; Sun, Yan1,2; Shi, Qing-Hong1,2,3
2015-11-15
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume103Issue:NOVPages:122-129
AbstractThe static adsorption capacity for proteins is regarded as the benchmark for achieving high binding capacity in protein chromatography. This research provided an efficient and controlled approach for the synthesis of ion-exchange beads with high adsorption capacity via surface-initiated atom transfer radical polymerization (SI-ATRP) by grafting methacryloxyethyltrimethyl ammonium chloride to a micron-sized poly(glycidyl methacrylate) (pGMA) matrix. The result showed that grafting of polymeric ion-exchange groups significantly increased the ionic capacity of the micron-sized beads and the adsorption density for gamma-globulin. The adsorption capacity for gamma-globulin could be optimized by tuning the chain length and the density of the charged polymer grafted onto the beads. The maximal saturated adsorption capacity for gamma-globulin reached 808 mg/g wet beads, which is nine times higher than that of non-grafted-ion-exchange beads. In a batch kinetics experiment, gamma-globulin achieved adsorption equilibriums rapidly (within 10 min). This finding indicates that the protein was involved in a distinct adsorption mechanism during the protein binding to surface-grafted polymer chains. The results demonstrated that polymer-grafted ion-exchange beads prepared by SI-ATRP possess a high adsorption capacity and rapid kinetics. Thus, these beads are expected to be highly useful as novel chromatographic materials for protein purification. (C) 2015 Elsevier B.V. All rights reserved.
KeywordBioseparation Polyclonal Antibodies Chromatography Adsorption High Capacity Polymer Grafting
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.bej.2015.07.010
Indexed BySCI
Language英语
WOS KeywordENTROPIC INTERACTION CHROMATOGRAPHY ; ADSORPTION ; TRANSPORT ; RESINS ; MEDIA ; ATRP ; COPOLYMERS ; KINETICS ; CATALYST ; BEHAVIOR
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
WOS IDWOS:000362137400014
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19616
Collection生化工程国家重点实验室
Affiliation1.Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, Tianjin 300072, Peoples R China
2.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Syst Bioengn, Tianjin 300072, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Li, Shu,Sun, Yan,Shi, Qing-Hong. Fabrication of high-capacity protein ion-exchangers with polymeric ion-exchange groups grafted onto micron-sized beads by atom transfer radical polymerization[J]. BIOCHEMICAL ENGINEERING JOURNAL,2015,103(NOV):122-129.
APA Li, Shu,Sun, Yan,&Shi, Qing-Hong.(2015).Fabrication of high-capacity protein ion-exchangers with polymeric ion-exchange groups grafted onto micron-sized beads by atom transfer radical polymerization.BIOCHEMICAL ENGINEERING JOURNAL,103(NOV),122-129.
MLA Li, Shu,et al."Fabrication of high-capacity protein ion-exchangers with polymeric ion-exchange groups grafted onto micron-sized beads by atom transfer radical polymerization".BIOCHEMICAL ENGINEERING JOURNAL 103.NOV(2015):122-129.
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