CAS OpenIR  > 生化工程国家重点实验室
Preparation of a novel polymer monolith with high loading capacity by grafting block poly(PEGA-mPEGA) for high-efficiency solid phase synthesis
Zhang, Rongyue1,2; Li, Qiang2; Ji, Dekun1; Pan, Yiting1; Xu, Bo1; Huang, Yongdong2; Zhao, Lan2
2015-09-01
Source PublicationREACTIVE & FUNCTIONAL POLYMERS
ISSN1381-5148
Volume94Issue:SEPPages:63-69
Abstract

The polymer monolith for solid-phase synthesis with high efficiency was prepared through in situ copolymerization of chloromethylstyrene and ethylene glycol dimethacrylate (PCMS-EDMA). The obtained monolith was grafted by two kinds of poly( ethylene glycol) acrylate oligomer, poly(ethylene glycol) acrylate (PEGA) and poly(ethylene glycol) methyl ether acrylate (mPEGA). The monolith was grafted via activators generated by electron transfer atom transfer radical polymerization (AGET ATRP) with the increased number of functional groups (-OH). About 0.61-0.81 mmol/g hydroxyl group resulted from side groups in each grafting polymer chain. PmPEGA in the grafting block copolymer chains can increase the distance between the adjacent reactive sites of PEGA (-OH) in each polymer chain. Therefore, the grafted monoliths with the block copolymer of PEGA-co-mPEGA can give high yield (85%) and purity (93%) of the crude peptide (a difficult sequence-acyl carrier protein fragment 65-74) under the condition of high loading capacity (0.76 mmol/g). These results were higher than those by the grafted monolith with only polymer of PEGA (72% and 81%, respectively) and commercial Wang resin (43% and 39%, respectively). The synthetic efficiency on the grafted monolith with block copolymer in the continuous flow technique was 5-6 folds higher than Wang resins in the manual operation conditions. (C) 2015 Elsevier B.V. All rights reserved.

KeywordPorous Rigid Monolith Solid Phase Synthesis Block Copolymer Atom Transfer Radical Polymerization Continuous Flow Technique
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
DOI10.1016/j.reactfunctpolym.2015.08.002
Indexed BySCI
Language英语
WOS KeywordPEPTIDE-SYNTHESIS ; AFFINITY SEPARATION ; SUPPORTS ; COLUMNS ; POROGEN ; RESIN ; CHROMATOGRAPHY ; MORPHOLOGY
WOS Research AreaChemistry ; Engineering ; Polymer Science
WOS SubjectChemistry, Applied ; Engineering, Chemical ; Polymer Science
WOS IDWOS:000361925500008
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19620
Collection生化工程国家重点实验室
Affiliation1.Beijing Inst Petrochem Technol, Dept Chem Engn, Beijing 102617, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Rongyue,Li, Qiang,Ji, Dekun,et al. Preparation of a novel polymer monolith with high loading capacity by grafting block poly(PEGA-mPEGA) for high-efficiency solid phase synthesis[J]. REACTIVE & FUNCTIONAL POLYMERS,2015,94(SEP):63-69.
APA Zhang, Rongyue.,Li, Qiang.,Ji, Dekun.,Pan, Yiting.,Xu, Bo.,...&Zhao, Lan.(2015).Preparation of a novel polymer monolith with high loading capacity by grafting block poly(PEGA-mPEGA) for high-efficiency solid phase synthesis.REACTIVE & FUNCTIONAL POLYMERS,94(SEP),63-69.
MLA Zhang, Rongyue,et al."Preparation of a novel polymer monolith with high loading capacity by grafting block poly(PEGA-mPEGA) for high-efficiency solid phase synthesis".REACTIVE & FUNCTIONAL POLYMERS 94.SEP(2015):63-69.
Files in This Item:
File Name/Size DocType Version Access License
Preparation of a nov(965KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Rongyue]'s Articles
[Li, Qiang]'s Articles
[Ji, Dekun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Rongyue]'s Articles
[Li, Qiang]'s Articles
[Ji, Dekun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Rongyue]'s Articles
[Li, Qiang]'s Articles
[Ji, Dekun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.