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Molecular shape and immunogenicity of meningococcal polysaccharide group A conjugate vaccine
Xu, Mengfang1,3; Xing, Xueqing2; Wu, Zhonghua2; Du, Yuguang1; Hu, Tao1
2015-10-26
Source PublicationVACCINE
ISSN0264-410X
Volume33Issue:43Pages:5815-5821
AbstractNeisseria meningitidis is a leading cause of severe bacterial infections in infants and young children. As a major virulence factor, meningococcal capsular polysaccharide (PS) is poorly immunogenic and generally does not induce immunological memory. Conjugation of PS with a carrier protein can significantly increase the PS-specific immunogenicity and induce immunological memory. It is well known that the molecular shape/size of the conjugate vaccine is important for its immunogenicity. However, little is known about the molecular shape/size of the meningococcal conjugate vaccine. A meningococcal PS ovalbumin (OVA) conjugate vaccine was prepared using cystamine as linker. Four components (P1-P4) with different molecular size were fractionated from the conjugate. Small angle X-ray scattering (SAXS) analysis revealed that the conjugate vaccine exhibited a rod-like shape similar to virus-like particles. PS-specific immunogenicity of the conjugate vaccine was related to its molecular shape and increased as a function of its molecular size. Thus, the present study provides a three-dimensional shape of the conjugate vaccine and helps to identify optimal design of a potent meningococcal conjugate vaccine. (C) 2015 Elsevier Ltd. All rights reserved.
KeywordNeisseria Meningitidis Polysaccharide Conjugate Vaccine Saxs Molecular Shape
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.vaccine.2015.09.021
Indexed BySCI
Language英语
WOS KeywordSOLUTION SCATTERING ; IMMUNE-RESPONSES ; RESOLUTION ; PROTEINS ; CARRIER ; DESIGN
WOS Research AreaImmunology ; Research & Experimental Medicine
WOS SubjectImmunology ; Medicine, Research & Experimental
Funding OrganizationBeijing Natural Science Foundation(7142104) ; National Natural Science Foundation of China(20906095 ; National High-tech R&D Program of Ministry of Sciences and Technology of China (863 Program)(2014AA093604) ; STS Project of Chinese Academy of Sciences(KFJ-EW-STS-027 ; 11405199 ; KFJ-EW-STS-098) ; 81402861)
WOS IDWOS:000363824600013
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19755
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Xu, Mengfang,Xing, Xueqing,Wu, Zhonghua,et al. Molecular shape and immunogenicity of meningococcal polysaccharide group A conjugate vaccine[J]. VACCINE,2015,33(43):5815-5821.
APA Xu, Mengfang,Xing, Xueqing,Wu, Zhonghua,Du, Yuguang,&Hu, Tao.(2015).Molecular shape and immunogenicity of meningococcal polysaccharide group A conjugate vaccine.VACCINE,33(43),5815-5821.
MLA Xu, Mengfang,et al."Molecular shape and immunogenicity of meningococcal polysaccharide group A conjugate vaccine".VACCINE 33.43(2015):5815-5821.
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