CAS OpenIR  > 湿法冶金清洁生产技术国家工程实验室
Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles
Yu, Jiemiao1,2,3; Yang, Liangrong2; Liang, Xiangfeng2; Dong, Tingting2,3; Liu, Huizhou1,2
2015-11-01
Source PublicationTALANTA
ISSN0039-9140
Volume144Issue:NOVPages:312-317
AbstractIn the protein separation, adsorption and desorption of target protein have been using different buffer condition. Different buffer will change the structure and activity of target protein in some cases. This work describes the use of different wavelength light for remote regulation of adsorption and desorption of target protein in the same buffer solutions. A dynamic system that captured and released protein in response to light is reported. Matrix gold nanoparticles and light-responsive affinity ligand comprising thrombin aptamer (APT15), polyethylene glycol linker, and azobenzene-modified complementary sequence were used. UV light induced a trans-cis isomerization of the azobenzene that destabilized the duplex of aptamer and azobenzene-modified complementary sequence, resulting in thrombin binding to aptamer sequence. Visible light irradiation resulted in DNA duplex rehybridization and thrombin released. Our work demonstrates that different light wavelengths effectively regulated the adsorption and desorption of thrombin in the same buffer, and this system also can capture and release prothrombin from plasma with different wavelength light. Furthermore, this method can be widely applied to a variety of different protein separation process. (C) 2015 Elsevier B.V. All rights reserved.
KeywordPhotoresponsive Thrombin Aptamer Capture Release
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1016/j.talanta.2015.06.053
Indexed BySCI
Language英语
WOS KeywordAZOBENZENE-TETHERED DNA ; AFFINITY-CHROMATOGRAPHY ; CAPILLARY CHROMATOGRAPHY ; COMBINATORIAL CHEMISTRY ; DUPLEX FORMATION ; PHOTOREGULATION ; TRANSCRIPTION ; POLYMERASE ; SELECTION ; BIND
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
Funding OrganizationNational Natural Science Foundation of China(21106162) ; National Key Natural Science Foundation of China(21136009)
WOS IDWOS:000363346200044
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19758
Collection湿法冶金清洁生产技术国家工程实验室
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,et al. Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles[J]. TALANTA,2015,144(NOV):312-317.
APA Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,Dong, Tingting,&Liu, Huizhou.(2015).Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles.TALANTA,144(NOV),312-317.
MLA Yu, Jiemiao,et al."Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles".TALANTA 144.NOV(2015):312-317.
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