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Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases
Zhang, Chenxu1,2; Zhang, Rongyue3; Li, Qiang1; Huang, Yongdong1; Zhao, Lan1; Su, Zhiguo1; Gong, Fangling1; Lv, Zheng4; Song, Hongyan4; Li, Wei4; Yuan, Qipeng2; Ma, Guanghui1
2015-11-05
Source PublicationSEPARATION AND PURIFICATION TECHNOLOGY
ISSN1383-5866
Volume154Issue:NOVPages:351-358
AbstractOctreotide, a disulfide-cyclized eight amino acids long analogue of somatostatin, has been used to treat the symptoms associated with metastatic carcinoid tumors (flushing and diarrhea), and vasoactive intestinal peptide secreting adenomas (watery diarrhea). As composed by D-amino acids, C-terminal alcohol, and disulfide bond, octreotide can be only prepared using chemistry synthesis method, which increases difficulty of the traditional reversed-phase separation. In this paper, we report a very fast separation of octreotide from synthetic peptide crude mixtures on a poly(styrene co-divinylbenzene) (PSt)-based reversed phases prepared by a quick membrane emulsification process. In general, fourier-transformed infrared resonance (FT-IR) spectroscopy, reversed-phase high performance liquid chromatography (HPLC) combined with electrospray ionisation mass spectrometry (ESI-MS), and nuclear magnetic resonance (NMR) spectroscopy are routinely used for analyses of resultant peptides. Under the optimized conditions, the octreotide is purified from 42.89% to 99.99%. Consequently, the poly(styrene co-divinylbenzene)-based phase can provide more flexibility to improve separation efficiency for the octreotide from the synthetic peptide crude mixtures. (C) 2015 Elsevier B.V. All rights reserved.
KeywordOctreotide Reversed-phase Separation Poly(Styrene-co-divinylbenzene)
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1016/j.seppur.2015.09.050
Indexed BySCI
Language英语
WOS KeywordAMINO-ACID ; PURIFICATION ; POLYSTYRENE ; PROTEINS ; HPLC ; SILICA ; COLUMNS ; RESINS
WOS Research AreaEngineering
WOS SubjectEngineering, Chemical
Funding OrganizationNational Natural Science Foundation of China(21206175 ; National Key Basic Research Program of China(2013BAB01B03 ; 51103158 ; 2014AA021005 ; 21476241 ; 2014AA021006) ; 21306206 ; 51403213)
WOS IDWOS:000364245500043
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/19808
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China
3.Beijing Inst Petrochem Technol, Dept Chem Engn, Beijing 102617, Peoples R China
4.Capital Normal Univ, Dept Chem, Beijing 100048, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Chenxu,Zhang, Rongyue,Li, Qiang,et al. Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2015,154(NOV):351-358.
APA Zhang, Chenxu.,Zhang, Rongyue.,Li, Qiang.,Huang, Yongdong.,Zhao, Lan.,...&Ma, Guanghui.(2015).Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases.SEPARATION AND PURIFICATION TECHNOLOGY,154(NOV),351-358.
MLA Zhang, Chenxu,et al."Rapid octreotide separation from synthetic peptide crude mixtures by chromatography on poly(styrene-co-divinylbenzene)-based reversed phases".SEPARATION AND PURIFICATION TECHNOLOGY 154.NOV(2015):351-358.
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