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Directing membrane chromatography to manufacture alpha(1)-antitrypsin from human plasma fraction IV
Fan, Jinxin1,2; Luo, Jianquan1; Song, Weijie1; Chen, Xiangrong1; Wan, Yinhua1
2015-12-04
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1423Issue:DECPages:63-70
AbstractThe surging demand for plasma proteins, mainly driven by the growing market and the development of new therapeutic indications, is promoting manufacturers to improve the throughput of plasma proteins. Due to the inherent convective mass transfer, membrane chromatography has been proved to be an efficient approach for extracting a small amount of target proteins from large-volume feed. In this study, ai-antitrypsin (AAT) was extracted from human plasma fraction IV by a two-step membrane chromatography. An anion-exchange membrane chromatography (AEMC) was used to capture the plasma proteins in bind/elute mode, and the obtained effluent was further polished by a hydrophobic interaction membrane chromatography (HIMC) in flow-through mode. Under optimal conditions, the recovery and purity of MT achieved 87.0% and 0.58 MT/protein (g/g) by AEMC, respectively. After the precise polishing by HIMC, the purity of AAT was 1.22 MT/protein (g/g). The comparison results showed that membrane chromatography outperformed column chromatography in both steps because of its high throughput. This two-step membrane chromatography could obtain an MT recovery of 83.3% and an activity recovery of 91.4%. The outcome of this work not only offers an alternative process for protein purification from plasma, but also provides guidelines for manufacturing product from a large-volume feed with multi-components by membrane chromatography. (C) 2015 Elsevier B.V. All rights reserved.
KeywordMembrane Chromatography Plasma Protein Alpha(1)-antitrypsin Ion-exchange Hydrophobic Interaction Membrane Adsorber
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2015.10.050
Indexed BySCI
Language英语
WOS KeywordION-EXCHANGE CHROMATOGRAPHY ; HYBRID BIOSEPARATION TECHNIQUE ; ALPHA(1) PROTEINASE-INHIBITOR ; SCALE ANTIBODY PURIFICATION ; MONOCLONAL-ANTIBODY ; ALPHA-1-ANTITRYPSIN ; CONCENTRATE ; WHEY ; STEP ; SEPARATION
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
Funding OrganizationNational High Technology Research and Development Program of China (863 Program)(2012AA021903) ; "100 Talents Program" of Institute of Process Engineering, Chinese Academy of Sciences
WOS IDWOS:000366073000008
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/20143
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Fan, Jinxin,Luo, Jianquan,Song, Weijie,et al. Directing membrane chromatography to manufacture alpha(1)-antitrypsin from human plasma fraction IV[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1423(DEC):63-70.
APA Fan, Jinxin,Luo, Jianquan,Song, Weijie,Chen, Xiangrong,&Wan, Yinhua.(2015).Directing membrane chromatography to manufacture alpha(1)-antitrypsin from human plasma fraction IV.JOURNAL OF CHROMATOGRAPHY A,1423(DEC),63-70.
MLA Fan, Jinxin,et al."Directing membrane chromatography to manufacture alpha(1)-antitrypsin from human plasma fraction IV".JOURNAL OF CHROMATOGRAPHY A 1423.DEC(2015):63-70.
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