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Directing membrane chromatography to manufacture alpha(1)-antitrypsin from human plasma fraction IV
Fan, Jinxin1,2; Luo, Jianquan1; Song, Weijie1; Chen, Xiangrong1; Wan, Yinhua1
2015-12-04
发表期刊JOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
卷号1423期号:DEC页码:63-70
摘要The surging demand for plasma proteins, mainly driven by the growing market and the development of new therapeutic indications, is promoting manufacturers to improve the throughput of plasma proteins. Due to the inherent convective mass transfer, membrane chromatography has been proved to be an efficient approach for extracting a small amount of target proteins from large-volume feed. In this study, ai-antitrypsin (AAT) was extracted from human plasma fraction IV by a two-step membrane chromatography. An anion-exchange membrane chromatography (AEMC) was used to capture the plasma proteins in bind/elute mode, and the obtained effluent was further polished by a hydrophobic interaction membrane chromatography (HIMC) in flow-through mode. Under optimal conditions, the recovery and purity of MT achieved 87.0% and 0.58 MT/protein (g/g) by AEMC, respectively. After the precise polishing by HIMC, the purity of AAT was 1.22 MT/protein (g/g). The comparison results showed that membrane chromatography outperformed column chromatography in both steps because of its high throughput. This two-step membrane chromatography could obtain an MT recovery of 83.3% and an activity recovery of 91.4%. The outcome of this work not only offers an alternative process for protein purification from plasma, but also provides guidelines for manufacturing product from a large-volume feed with multi-components by membrane chromatography. (C) 2015 Elsevier B.V. All rights reserved.
关键词Membrane Chromatography Plasma Protein Alpha(1)-antitrypsin Ion-exchange Hydrophobic Interaction Membrane Adsorber
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2015.10.050
收录类别SCI
语种英语
关键词[WOS]ION-EXCHANGE CHROMATOGRAPHY ; HYBRID BIOSEPARATION TECHNIQUE ; ALPHA(1) PROTEINASE-INHIBITOR ; SCALE ANTIBODY PURIFICATION ; MONOCLONAL-ANTIBODY ; ALPHA-1-ANTITRYPSIN ; CONCENTRATE ; WHEY ; STEP ; SEPARATION
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
项目资助者National High Technology Research and Development Program of China (863 Program)(2012AA021903) ; "100 Talents Program" of Institute of Process Engineering, Chinese Academy of Sciences
WOS记录号WOS:000366073000008
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20143
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Fan, Jinxin,Luo, Jianquan,Song, Weijie,et al. Directing membrane chromatography to manufacture alpha(1)-antitrypsin from human plasma fraction IV[J]. JOURNAL OF CHROMATOGRAPHY A,2015,1423(DEC):63-70.
APA Fan, Jinxin,Luo, Jianquan,Song, Weijie,Chen, Xiangrong,&Wan, Yinhua.(2015).Directing membrane chromatography to manufacture alpha(1)-antitrypsin from human plasma fraction IV.JOURNAL OF CHROMATOGRAPHY A,1423(DEC),63-70.
MLA Fan, Jinxin,et al."Directing membrane chromatography to manufacture alpha(1)-antitrypsin from human plasma fraction IV".JOURNAL OF CHROMATOGRAPHY A 1423.DEC(2015):63-70.
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