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Development of a thermally stable formulation for L-asparaginase storage in aqueous conditions
Fu, Yankai1,2; Wu, Yanzhuo3; Wei, Yuping1; Chen, Xianhua3; Xu, Jiandong1; Xu, Xia1
2015-12-01
Source PublicationJOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
ISSN1381-1177
Volume122Issue:DECPages:8-14
AbstractL-Asparaginase is an enzyme broadly used in pharmaceutical and food industry. Freeze-drying of L-asparaginase is time and energy consuming, and can cause enzyme activity loss. Furthermore, temperature excursion during transport and storage may ruin its activity as well. Hence, it is necessary to develop a thermostable liquid formulation of L-asparaginase. In this study, a thermostable liquid formulation using glycine as a protecting excipient was developed based on the screening tests. The presence of glycine in the citrate-phosphate buffer significantly increased the melting point by 4 degrees C and further dramatically slowed down the denaturation of L-asparaginase at 45 and 50 degrees C No subunit dissociation of L-asparaginase was detected by native-polyacrylamide gel electrophoresis, indicating that the denaturation of L-asparaginase was mainly caused by the changes in the secondary structure, confirmed by the circular dichroism data. This novel thermostable liquid formulation could offer significant promise for long-term storage of L-asparaginase in aqueous conditions. (C) 2015 Elsevier B.V. All rights reserved.
KeywordL-asparaginase Enzyme Activity Denaturation Thermostable Liquid Formulation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.molcatb.2015.08.019
Indexed BySCI
Language英语
WOS KeywordHIGHLY ACTIVATED SUPPORTS ; PROTEIN PHARMACEUTICALS ; MAILLARD REACTION ; ESCHERICHIA-COLI ; STABILITY ; ACRYLAMIDE ; THERMOSTABILITY ; INACTIVATION ; CAROTOVORA ; SUBSTRATE
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Physical
WOS IDWOS:000366078800002
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/20146
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Shuanglu Pharmaceut Co Ltd, Beijing 100043, Peoples R China
Recommended Citation
GB/T 7714
Fu, Yankai,Wu, Yanzhuo,Wei, Yuping,et al. Development of a thermally stable formulation for L-asparaginase storage in aqueous conditions[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2015,122(DEC):8-14.
APA Fu, Yankai,Wu, Yanzhuo,Wei, Yuping,Chen, Xianhua,Xu, Jiandong,&Xu, Xia.(2015).Development of a thermally stable formulation for L-asparaginase storage in aqueous conditions.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,122(DEC),8-14.
MLA Fu, Yankai,et al."Development of a thermally stable formulation for L-asparaginase storage in aqueous conditions".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 122.DEC(2015):8-14.
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