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Development of a thermally stable formulation for L-asparaginase storage in aqueous conditions
Fu, Yankai1,2; Wu, Yanzhuo3; Wei, Yuping1; Chen, Xianhua3; Xu, Jiandong1; Xu, Xia1
2015-12-01
发表期刊JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
ISSN1381-1177
卷号122期号:DEC页码:8-14
摘要L-Asparaginase is an enzyme broadly used in pharmaceutical and food industry. Freeze-drying of L-asparaginase is time and energy consuming, and can cause enzyme activity loss. Furthermore, temperature excursion during transport and storage may ruin its activity as well. Hence, it is necessary to develop a thermostable liquid formulation of L-asparaginase. In this study, a thermostable liquid formulation using glycine as a protecting excipient was developed based on the screening tests. The presence of glycine in the citrate-phosphate buffer significantly increased the melting point by 4 degrees C and further dramatically slowed down the denaturation of L-asparaginase at 45 and 50 degrees C No subunit dissociation of L-asparaginase was detected by native-polyacrylamide gel electrophoresis, indicating that the denaturation of L-asparaginase was mainly caused by the changes in the secondary structure, confirmed by the circular dichroism data. This novel thermostable liquid formulation could offer significant promise for long-term storage of L-asparaginase in aqueous conditions. (C) 2015 Elsevier B.V. All rights reserved.
关键词L-asparaginase Enzyme Activity Denaturation Thermostable Liquid Formulation
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.molcatb.2015.08.019
收录类别SCI
语种英语
关键词[WOS]HIGHLY ACTIVATED SUPPORTS ; PROTEIN PHARMACEUTICALS ; MAILLARD REACTION ; ESCHERICHIA-COLI ; STABILITY ; ACRYLAMIDE ; THERMOSTABILITY ; INACTIVATION ; CAROTOVORA ; SUBSTRATE
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemistry & Molecular Biology ; Chemistry, Physical
WOS记录号WOS:000366078800002
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20146
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Shuanglu Pharmaceut Co Ltd, Beijing 100043, Peoples R China
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GB/T 7714
Fu, Yankai,Wu, Yanzhuo,Wei, Yuping,et al. Development of a thermally stable formulation for L-asparaginase storage in aqueous conditions[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2015,122(DEC):8-14.
APA Fu, Yankai,Wu, Yanzhuo,Wei, Yuping,Chen, Xianhua,Xu, Jiandong,&Xu, Xia.(2015).Development of a thermally stable formulation for L-asparaginase storage in aqueous conditions.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,122(DEC),8-14.
MLA Fu, Yankai,et al."Development of a thermally stable formulation for L-asparaginase storage in aqueous conditions".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 122.DEC(2015):8-14.
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