Structurally related ganoderic acids induce apoptosis in human cervical cancer He La cells: Involvement of oxidative stress and antioxidant protective system
Liu, Ru-Ming1; Li, Ying-Bo2; Liang, Xiang-Feng2; Liu, Hui-Zhou2; Xiao, Jian-Hui1; Zhong, Jian-Jiang3,4
2015-10-05
发表期刊CHEMICO-BIOLOGICAL INTERACTIONS
ISSN0009-2797
卷号240期号:OCT页码:134-144
摘要

Ganoderic acids (GAs) produced by Ganoderma lucidum possess anticancer activities with the generation of reactive oxygen species (ROS). However, the role of oxidative stress in apoptotic process induced by GAs is still undefined. In this study, the effects of four structurally related GAs, i.e. GA-T, GA-Mk, and two deacetylated derivatives of GA-T (GA-T1 and GA-T2) on the antioxidant defense system and induced apoptosis in cervical cancer cells HeLa were investigated in vitro. Our results indicated that the tested GAs (5-40 mu M) induced apoptotic cell death through mitochondrial membrane potential decrease and activation of caspase-9 and caspase-3. Furthermore. GAs increased the generation of intracellular ROS and attenuated antioxidant defense system by decreasing glutathione (GSH) level, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities. The above effects were remarkably blocked by the exogenous antioxidants, i.e. N-acetylcysteine, catalase and diphenyleneiodonium chloride. The potency of the four GAs toward induced apoptosis, generation of ROS and suppression of antioxidant defense system was in the order of: GA-T > GA-Mk approximate to GA-T1 > GA-T2 in HeLa cells. These findings suggest that GAs induced mitochondria-dependent cell apoptosis in HeLa cells are mediated via enhancing oxidative stress and depressing antioxidant defense. Additionally, the acetylation of hydroxyl groups in GAs may contribute to their pro-oxidant activities and cytotoxicity, which is helpful to the development of novel chemotherapy agents. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

关键词Ganoderma Lucidum Ganoderic Acid Reactive Oxygen Species Acetylation Cell Apoptosis Antioxidant Defense System
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.cbi.2015.08.005
收录类别SCI
语种英语
关键词[WOS]MITOCHONDRIA-MEDIATED APOPTOSIS ; OXYGEN SPECIES ROS ; LUCIDUM MYCELIA ; IN-VITRO ; CARCINOMA-CELLS ; EXPRESSION ; ENHANCEMENT ; TRITERPENES ; DERIVATIVES ; BIOACTIVITY
WOS研究方向Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Toxicology
WOS类目Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Toxicology
项目资助者National Natural Science Foundation of China(30270038 ; Shanghai Science & Technology Commission(054319933 ; Guizhou High-Level Innovative Talent Support Program(QKH-RC-20154028) ; Program for Innovative Research Team in Guizhou Province(QKH-RCTD-20134035) ; Honghuagang district Science and Technology Project, Zunyi(2014-12) ; 20776084) ; 08DZ1971900)
WOS记录号WOS:000366950400014
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20185
专题湿法冶金清洁生产技术国家工程实验室
作者单位1.Zunyi Med Univ, Affiliated Hosp, Guizhou Ctr Translat Med, Zunyi 563000, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
3.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Lab Mol Biochem Engn, Shanghai 200240, Peoples R China
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GB/T 7714
Liu, Ru-Ming,Li, Ying-Bo,Liang, Xiang-Feng,et al. Structurally related ganoderic acids induce apoptosis in human cervical cancer He La cells: Involvement of oxidative stress and antioxidant protective system[J]. CHEMICO-BIOLOGICAL INTERACTIONS,2015,240(OCT):134-144.
APA Liu, Ru-Ming,Li, Ying-Bo,Liang, Xiang-Feng,Liu, Hui-Zhou,Xiao, Jian-Hui,&Zhong, Jian-Jiang.(2015).Structurally related ganoderic acids induce apoptosis in human cervical cancer He La cells: Involvement of oxidative stress and antioxidant protective system.CHEMICO-BIOLOGICAL INTERACTIONS,240(OCT),134-144.
MLA Liu, Ru-Ming,et al."Structurally related ganoderic acids induce apoptosis in human cervical cancer He La cells: Involvement of oxidative stress and antioxidant protective system".CHEMICO-BIOLOGICAL INTERACTIONS 240.OCT(2015):134-144.
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