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Characterization of a new family 75 chitosanase from Aspergillus sp W-2
Zhang, Jianping1,2; Cao, Hailong1; Li, Shuguang1; Zhao, Yong1; Wang, Wenxia1; Xu, Qingsong1; Du, Yuguang1,3; Yin, Heng1
2015-11-01
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
卷号81期号:NOV页码:362-369
摘要A new chitosanase gene of glycoside hydrolase (GH) family 75, csnw2, was cloned from an isolated strain Aspergillus sp. W-2 (CGMCC7018). The mature CsnW2 protein, fused to His-tag at C-terminus, was expressed in Pichia pastoris X-33 and purified with the affinity chromatography of Ni2+-NTA. The novel recombinant CsnW2 showed maximal activity with chitosan at pH 6.0 and 55 degrees C. Moreover, it had good pH stability and thermostability at a broad pH range of 3.0-10.0 and a temperature range of 30-70 degrees C, respectively. The enzymatic activity of the CsnW2 could be significantly enhanced by Ca2+, Mn2+ and Mg2+ at a concentration of 1 mM, but strongly inhibited by Fe2+, Zn2+, Ge2+, Ni2+ and Cu2+ above 1 mM. The CsnW2 showed specific hydrolytic activity against chitosan and preferred to hydrolyze chitosan with high degree of deacetylation. The main products of chtiosan (92% deacetylation) were chitosan oligosaccharides (COS) with degree of polymerization (DP) arranging from 2 to 6. Combined with the hydrolysis of COS from DP2 to DP6, CsnW2 was considered to be an endo-acting chitosanase. (C) 2015 Elsevier B.V. All rights reserved.
关键词Chitosanase ASp.rgillus Sp. Glycoside Hydrolyase Family 75
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.ijbiomac.2015.08.026
收录类别SCI
语种英语
关键词[WOS]OLIGOSACCHARIDES ; FUMIGATUS ; CHITOOLIGOSACCHARIDES ; MECHANISM ; CLONING ; CHITIN
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
项目资助者Chinese High-Tech Research and Development Program(2011AA10A205 ; National Key Laboratory of Biochemical Engineering(2012KF-06) ; Chinese Academy of Sciences(KSZD-EW-Z-015-2) ; 2012AA021501 ; 2014AA093604)
WOS记录号WOS:000367408300044
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20247
专题研究所(批量导入)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Beijing 10090, Peoples R China
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Zhang, Jianping,Cao, Hailong,Li, Shuguang,et al. Characterization of a new family 75 chitosanase from Aspergillus sp W-2[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,81(NOV):362-369.
APA Zhang, Jianping.,Cao, Hailong.,Li, Shuguang.,Zhao, Yong.,Wang, Wenxia.,...&Yin, Heng.(2015).Characterization of a new family 75 chitosanase from Aspergillus sp W-2.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,81(NOV),362-369.
MLA Zhang, Jianping,et al."Characterization of a new family 75 chitosanase from Aspergillus sp W-2".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 81.NOV(2015):362-369.
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