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(1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study
Fu, Yunbin1,2; Wang, Mengyu1; Wang, Wenxia1; Tuo, Yaqin3; Guo, Zhimou4; Du, Yuguang5; Yin, Heng1
2015-11-01
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
卷号81期号:NOV页码:1069-1073
摘要In order to produce highly purified (1 -> 3)-beta-D-glucan oligosaccharides ((1 -> 3)-beta-D-GOS) monomers, a hydrophilic interaction liquid chromatography (HILIC) system with X-Amide stationary phase was performed. Nine (1 -> 3)-beta-D-GOS monomers with degree of polymerization (DP) from 2 to 10 were successfully separated. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) demonstrated that these monomers were with high purity. Furthermore, a hydrogen peroxide (H2O2) online detection method was established to monitor H2O2 releases in tobacco cells. This is the first report on nine consecutive (1 -> 3)-beta-D-GOS monomers purification and its effect upon H2O2-releasing in plants. Itwas found that (1 -> 3)-beta-D-GOS monomers with higher DP induced stronger defense responses in plants, which will pave the way for elucidating the relationship between (1 -> 3)-beta-D-GOS and biological activities. (C) 2015 Elsevier B.V. All rights reserved.
关键词(1 -> 3)-beta-d-glucan Oligosaccharides Monomer Degree Of Polymerization Hydrogen Peroxide Tobacco
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.ijbiomac.2015.09.028
收录类别SCI
语种英语
关键词[WOS]TOBACCO SUSPENSION CELLS ; BETA-1,3 GLUCAN SULFATE ; DEFENSE RESPONSES ; PLASMOPARA-VITICOLA ; RESISTANCE ; GRAPEVINE ; DEATH ; OLIGOGALACTURONIDES ; CHROMATOGRAPHY ; SEPARATION
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
项目资助者Hi-Tech Research and Development Program of China(2012AA021501 ; Natural Science Foundation of China(31370811) ; Youth Innovation Promotion Association, Chinese Academy of Sciences (CAS) ; 2011AA10A205)
WOS记录号WOS:000367408300133
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20251
专题研究所(批量导入)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycobiotechnol Grp, Dalian 116023, Liaoning, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
3.Dalian Glycobio Co Ltd, Dalian 116023, Liaoning, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Separat Mat & Separat Method Grp, Dalian 116023, Liaoning, Peoples R China
5.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
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Fu, Yunbin,Wang, Mengyu,Wang, Wenxia,et al. (1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,81(NOV):1069-1073.
APA Fu, Yunbin.,Wang, Mengyu.,Wang, Wenxia.,Tuo, Yaqin.,Guo, Zhimou.,...&Yin, Heng.(2015).(1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,81(NOV),1069-1073.
MLA Fu, Yunbin,et al."(1 -> 3)-beta-D-Glucan oligosaccharides monomers purification and its H2O2 induction effect study".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 81.NOV(2015):1069-1073.
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