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Sensitivity of human embryonic stem cells to different conditions during cryopreservation
Xu, Yanqing1; Zhang, Liang1,2; Xu, Jiandong1; Wei, Yuping1; Xu, Xia1
2015-12-01
Source PublicationCRYOBIOLOGY
ISSN0011-2240
Volume71Issue:3Pages:486-492
AbstractLow cell recovery rate of human embryonic stem cells (hESCs) resulting from cryopreservation damages leads to the difficulty in their successful commercialization of clinical applications. Hence in this study, sensitivity of human embryonic stem cells (hESCs) to different cooling rates, ice seeding and cryoprotective agent (CPA) types was compared and cell viability and recovery after cryopreservation under different cooling conditions were assessed. Both extracellular and intracellular ice formation were observed. Reactive oxidative species (ROS) accumulation of hESCs was determined. Cryopreservation of hESCs at 1 degrees C/min with the ice seeding and at the theoretically predicted optimal cooling rate (TPOCR) led to lower level of intracellular ROS, and prevented irregular and big ice clump formation compared with cryopreservation at 1 degrees C/min. This strategy further resulted in a significant increase in the hESC recovery when glycerol and 1,2-propanediol were used as the CPAs, but no increase for Me2SO. hESCs after cryopreservation under all the tested conditions still maintained their pluripotency. Our results provide guidance for improving the hESC cryopreservation recovery through the combination of CPA type, cooling rate and ice seeding. (C) 2015 Elsevier Inc. All rights reserved.
KeywordHescs Cryopreservation Recovery Ice Formation Reactive Oxidative Species
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.cryobiol.2015.10.151
Indexed BySCI
Language英语
WOS KeywordMEMBRANE-PERMEABILITY ; INTRACELLULAR ICE ; FREEZING INJURY ; ROCK INHIBITOR ; HUMAN OOCYTES ; SURVIVAL ; PROTOCOLS ; APOPTOSIS ; KINETICS ; RECOVERY
WOS Research AreaLife Sciences & Biomedicine - Other Topics ; Physiology
WOS SubjectBiology ; Physiology
Funding OrganizationNational Natural Science Foundation of China(21176238) ; Chinese Academy of Sciences
WOS IDWOS:000367496800016
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/20252
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Xu, Yanqing,Zhang, Liang,Xu, Jiandong,et al. Sensitivity of human embryonic stem cells to different conditions during cryopreservation[J]. CRYOBIOLOGY,2015,71(3):486-492.
APA Xu, Yanqing,Zhang, Liang,Xu, Jiandong,Wei, Yuping,&Xu, Xia.(2015).Sensitivity of human embryonic stem cells to different conditions during cryopreservation.CRYOBIOLOGY,71(3),486-492.
MLA Xu, Yanqing,et al."Sensitivity of human embryonic stem cells to different conditions during cryopreservation".CRYOBIOLOGY 71.3(2015):486-492.
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