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Sensitivity of human embryonic stem cells to different conditions during cryopreservation
Xu, Yanqing1; Zhang, Liang1,2; Xu, Jiandong1; Wei, Yuping1; Xu, Xia1
2015-12-01
发表期刊CRYOBIOLOGY
ISSN0011-2240
卷号71期号:3页码:486-492
摘要Low cell recovery rate of human embryonic stem cells (hESCs) resulting from cryopreservation damages leads to the difficulty in their successful commercialization of clinical applications. Hence in this study, sensitivity of human embryonic stem cells (hESCs) to different cooling rates, ice seeding and cryoprotective agent (CPA) types was compared and cell viability and recovery after cryopreservation under different cooling conditions were assessed. Both extracellular and intracellular ice formation were observed. Reactive oxidative species (ROS) accumulation of hESCs was determined. Cryopreservation of hESCs at 1 degrees C/min with the ice seeding and at the theoretically predicted optimal cooling rate (TPOCR) led to lower level of intracellular ROS, and prevented irregular and big ice clump formation compared with cryopreservation at 1 degrees C/min. This strategy further resulted in a significant increase in the hESC recovery when glycerol and 1,2-propanediol were used as the CPAs, but no increase for Me2SO. hESCs after cryopreservation under all the tested conditions still maintained their pluripotency. Our results provide guidance for improving the hESC cryopreservation recovery through the combination of CPA type, cooling rate and ice seeding. (C) 2015 Elsevier Inc. All rights reserved.
关键词Hescs Cryopreservation Recovery Ice Formation Reactive Oxidative Species
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.cryobiol.2015.10.151
收录类别SCI
语种英语
关键词[WOS]MEMBRANE-PERMEABILITY ; INTRACELLULAR ICE ; FREEZING INJURY ; ROCK INHIBITOR ; HUMAN OOCYTES ; SURVIVAL ; PROTOCOLS ; APOPTOSIS ; KINETICS ; RECOVERY
WOS研究方向Life Sciences & Biomedicine - Other Topics ; Physiology
WOS类目Biology ; Physiology
项目资助者National Natural Science Foundation of China(21176238) ; Chinese Academy of Sciences
WOS记录号WOS:000367496800016
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20252
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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GB/T 7714
Xu, Yanqing,Zhang, Liang,Xu, Jiandong,et al. Sensitivity of human embryonic stem cells to different conditions during cryopreservation[J]. CRYOBIOLOGY,2015,71(3):486-492.
APA Xu, Yanqing,Zhang, Liang,Xu, Jiandong,Wei, Yuping,&Xu, Xia.(2015).Sensitivity of human embryonic stem cells to different conditions during cryopreservation.CRYOBIOLOGY,71(3),486-492.
MLA Xu, Yanqing,et al."Sensitivity of human embryonic stem cells to different conditions during cryopreservation".CRYOBIOLOGY 71.3(2015):486-492.
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