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Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization
Zhao, Xinwei1; Guan, Yueping1; Xia, Changfu1; Xia, Tingting2; Qiu, Xiaolin1; Wang, Chuhang1; Guo, Chen2
2016-03-10
Source PublicationJOURNAL OF APPLIED POLYMER SCIENCE
ISSN0021-8995
Volume133Issue:10Pages:43005
AbstractMicrometer-sized superparamagnetic poly(styrene-glycidyl methacrylate)/Fe3O4 spheres were synthesized by two-stage dispersion polymerization with modified hydrophobic Fe3O4 nanoparticles, styrene (St), and glycidyl methacrylate (GMA). The morphology and properties of the magnetic Fe3O4-P (St-GMA) microspheres were examined by scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry, thermogravimetric analysis, and attenuated total reflectance. The average size of the obtained magnetic microspheres was 1.50 mu m in diameter with a narrow size distribution, and the saturation magnetization of the magnetic microspheres was 8.23 emu/g. The magnetic Fe3O4-P (St-GMA) microspheres with immobilized iminodiacetic acid-Cu2+ groups were used to investigate the adsorption capacity and selectivity of the model proteins, bovine hemoglobin (BHb) and bovine serum albumin (BSA). We found that the adsorption capacity of BHb was as high as 190.66 mg/g of microspheres, which was 3.20 times greater than that of BSA, which was only 59.64 mg/g of microspheres as determined by high-performance liquid chromatography. With a rather low nonspecific adsorption, these microspheres have great potential for protein separation and purification applications. (C) 2015 Wiley Periodicals, Inc.
KeywordAdsorption Functionalization Of Polymers Properties And Characterization Proteins
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1002/app.43005
Indexed BySCI
Language英语
WOS KeywordEMULSION POLYMERIZATION ; MONODISPERSE ; PARTICLES ; NANOPARTICLES ; SEPARATION ; IMMOBILIZATION ; PURIFICATION
WOS Research AreaPolymer Science
WOS SubjectPolymer Science
Funding OrganizationNational Natural Science Foundation of China(51274035) ; National Basic Research Program of China(2013CB632600)
WOS IDWOS:000367852900003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/20260
Collection生化工程国家重点实验室
Affiliation1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Xinwei,Guan, Yueping,Xia, Changfu,et al. Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization[J]. JOURNAL OF APPLIED POLYMER SCIENCE,2016,133(10):43005.
APA Zhao, Xinwei.,Guan, Yueping.,Xia, Changfu.,Xia, Tingting.,Qiu, Xiaolin.,...&Guo, Chen.(2016).Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization.JOURNAL OF APPLIED POLYMER SCIENCE,133(10),43005.
MLA Zhao, Xinwei,et al."Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization".JOURNAL OF APPLIED POLYMER SCIENCE 133.10(2016):43005.
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