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Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization
Zhao, Xinwei1; Guan, Yueping1; Xia, Changfu1; Xia, Tingting2; Qiu, Xiaolin1; Wang, Chuhang1; Guo, Chen2
2016-03-10
发表期刊JOURNAL OF APPLIED POLYMER SCIENCE
ISSN0021-8995
卷号133期号:10页码:43005
摘要Micrometer-sized superparamagnetic poly(styrene-glycidyl methacrylate)/Fe3O4 spheres were synthesized by two-stage dispersion polymerization with modified hydrophobic Fe3O4 nanoparticles, styrene (St), and glycidyl methacrylate (GMA). The morphology and properties of the magnetic Fe3O4-P (St-GMA) microspheres were examined by scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry, thermogravimetric analysis, and attenuated total reflectance. The average size of the obtained magnetic microspheres was 1.50 mu m in diameter with a narrow size distribution, and the saturation magnetization of the magnetic microspheres was 8.23 emu/g. The magnetic Fe3O4-P (St-GMA) microspheres with immobilized iminodiacetic acid-Cu2+ groups were used to investigate the adsorption capacity and selectivity of the model proteins, bovine hemoglobin (BHb) and bovine serum albumin (BSA). We found that the adsorption capacity of BHb was as high as 190.66 mg/g of microspheres, which was 3.20 times greater than that of BSA, which was only 59.64 mg/g of microspheres as determined by high-performance liquid chromatography. With a rather low nonspecific adsorption, these microspheres have great potential for protein separation and purification applications. (C) 2015 Wiley Periodicals, Inc.
关键词Adsorption Functionalization Of Polymers Properties And Characterization Proteins
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
DOI10.1002/app.43005
收录类别SCI
语种英语
关键词[WOS]EMULSION POLYMERIZATION ; MONODISPERSE ; PARTICLES ; NANOPARTICLES ; SEPARATION ; IMMOBILIZATION ; PURIFICATION
WOS研究方向Polymer Science
WOS类目Polymer Science
项目资助者National Natural Science Foundation of China(51274035) ; National Basic Research Program of China(2013CB632600)
WOS记录号WOS:000367852900003
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20260
专题生化工程国家重点实验室
作者单位1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Lab Separat Sci & Engn, Beijing 100190, Peoples R China
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Zhao, Xinwei,Guan, Yueping,Xia, Changfu,et al. Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization[J]. JOURNAL OF APPLIED POLYMER SCIENCE,2016,133(10):43005.
APA Zhao, Xinwei.,Guan, Yueping.,Xia, Changfu.,Xia, Tingting.,Qiu, Xiaolin.,...&Guo, Chen.(2016).Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization.JOURNAL OF APPLIED POLYMER SCIENCE,133(10),43005.
MLA Zhao, Xinwei,et al."Preparation and characterization of magnetic poly(styrene-glycidyl methacrylate) microspheres for highly efficient protein adsorption by two-stage dispersion polymerization".JOURNAL OF APPLIED POLYMER SCIENCE 133.10(2016):43005.
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