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Enrichment and separation of chlorogenic acid from the extract of Eupatorium adenophorum Spreng by macroporous resin
Liu, Boyan1,2; Dong, Beitao1,2; Yuan, Xiaofan1; Kuang, Qirong1; Zhao, Qingsheng1; Yang, Mei3; Liu, Jie3; Zhao, Bing1
2016
发表期刊JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
卷号1008期号:JAN页码:58-64
摘要A simple and efficient chromatographic method for separation of chlorogenic acid from Eupatorium adenophorum Spreng extract was developed. The adsorption properties of nine macroporous resins were evaluated. NKA-II resin showed much better adsorption/desorption properties. The adsorption of chlorogenic acid on NKA-II resin at 25 degrees C was well fitted to Langmuir isotherm model and pseudo-second-order kinetic model. The dynamic adsorption and desorption experiments were carried out on columns packed with NKA-II resin to optimize the separation process. The content of chlorogenic acid in the product increased to 22.17%, with a recovery yield of 82.41%. (C) 2015 Elsevier B.V. All rights reserved.
关键词Nka-ii Macroporous Resin Chlorogenic Acid Eupatorium Adenophorum Spreng Separation
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.jchromb.2015.10.026
收录类别SCI
语种英语
关键词[WOS]SOUTHWEST CHINA ; CROFTON WEED ; ADSORPTION ; ADSORBENT ; RESOURCE ; PLANT
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
项目资助者Panzhihua Xiyu Biotech Co., Ltd. (Sichuan, China)
WOS记录号WOS:000368205500009
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20268
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Panzhihua Xiyu Biotech Co Ltd, Panzhihua 617400, Peoples R China
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Liu, Boyan,Dong, Beitao,Yuan, Xiaofan,et al. Enrichment and separation of chlorogenic acid from the extract of Eupatorium adenophorum Spreng by macroporous resin[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1008(JAN):58-64.
APA Liu, Boyan.,Dong, Beitao.,Yuan, Xiaofan.,Kuang, Qirong.,Zhao, Qingsheng.,...&Zhao, Bing.(2016).Enrichment and separation of chlorogenic acid from the extract of Eupatorium adenophorum Spreng by macroporous resin.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1008(JAN),58-64.
MLA Liu, Boyan,et al."Enrichment and separation of chlorogenic acid from the extract of Eupatorium adenophorum Spreng by macroporous resin".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1008.JAN(2016):58-64.
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