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Enrichment and separation of chlorogenic acid from the extract of Eupatorium adenophorum Spreng by macroporous resin
Liu, Boyan1,2; Dong, Beitao1,2; Yuan, Xiaofan1; Kuang, Qirong1; Zhao, Qingsheng1; Yang, Mei3; Liu, Jie3; Zhao, Bing1
2016
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume1008Issue:JANPages:58-64
AbstractA simple and efficient chromatographic method for separation of chlorogenic acid from Eupatorium adenophorum Spreng extract was developed. The adsorption properties of nine macroporous resins were evaluated. NKA-II resin showed much better adsorption/desorption properties. The adsorption of chlorogenic acid on NKA-II resin at 25 degrees C was well fitted to Langmuir isotherm model and pseudo-second-order kinetic model. The dynamic adsorption and desorption experiments were carried out on columns packed with NKA-II resin to optimize the separation process. The content of chlorogenic acid in the product increased to 22.17%, with a recovery yield of 82.41%. (C) 2015 Elsevier B.V. All rights reserved.
KeywordNka-ii Macroporous Resin Chlorogenic Acid Eupatorium Adenophorum Spreng Separation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.jchromb.2015.10.026
Indexed BySCI
Language英语
WOS KeywordSOUTHWEST CHINA ; CROFTON WEED ; ADSORPTION ; ADSORBENT ; RESOURCE ; PLANT
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
Funding OrganizationPanzhihua Xiyu Biotech Co., Ltd. (Sichuan, China)
WOS IDWOS:000368205500009
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/20268
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Panzhihua Xiyu Biotech Co Ltd, Panzhihua 617400, Peoples R China
Recommended Citation
GB/T 7714
Liu, Boyan,Dong, Beitao,Yuan, Xiaofan,et al. Enrichment and separation of chlorogenic acid from the extract of Eupatorium adenophorum Spreng by macroporous resin[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1008(JAN):58-64.
APA Liu, Boyan.,Dong, Beitao.,Yuan, Xiaofan.,Kuang, Qirong.,Zhao, Qingsheng.,...&Zhao, Bing.(2016).Enrichment and separation of chlorogenic acid from the extract of Eupatorium adenophorum Spreng by macroporous resin.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1008(JAN),58-64.
MLA Liu, Boyan,et al."Enrichment and separation of chlorogenic acid from the extract of Eupatorium adenophorum Spreng by macroporous resin".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1008.JAN(2016):58-64.
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