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Gas-assisted magnetic separation for the purification of proteins in batch systems
Liu, Zhini1,2; Yang, Liangrong2; Dong, Tingting2; Li, Wensong1,2; Sun, Xitong2; Zhu, Menghao2; Duan, Zhengkang1; Liu, Qingfen2; Liu, Huizhou2
2016-02-01
发表期刊PARTICUOLOGY
ISSN1674-2001
卷号24期号:FEB页码:170-176
摘要In this paper, gas-assisted magnetic separation (GAMS), a technique that combines magnetic separation with flotation, was investigated for the potential large-scale separation of proteins. The GAMS process includes adsorption of target proteins and magnetic separation to recover protein-loaded magnetic particles from the dilute biosuspension with the assistance of bubbles. Microsized ethylenediamine-functionalized poly(glycidyl methacrylate) superparamagnetic microspheres (MPMs) and bovine serum albumin (BSA) were used as a model system. The feasibility of GAMS for capturing BSA-loaded MPMs from an appropriate medium was shown. High recovery of BSA-loaded MPMs was obtained by simple adjustment of the initial solution pH without extra detergents and antifoaming agents. The GAMS conditions were consistent with the adsorption conditions, and no proteins were desorbed from the MPMs during this process. Under the optimal conditions, the separation rate and recovery percentage reached 410 mL/min and 98% in 0.61 min, respectively. Conformational changes of BSA during the GAMS process were investigated by fluorescence spectroscopy and circular dichroism spectrometry. (C) 2015 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.
关键词Adsorption Magnetic Separation Flotation Recovery Bsa Conformation
文章类型Article
WOS标题词Science & Technology ; Technology
DOI10.1016/j.partic.2014.12.015
收录类别SCI
语种英语
关键词[WOS]SUPERPARAMAGNETIC EXTRACTION ; ADSORPTION ; PARTICLES ; ADSORBENT ; FLOTATION ; NANOPARTICLES ; MICROSPHERES ; REMOVAL ; ALBUMIN
WOS研究方向Engineering ; Materials Science
WOS类目Engineering, Chemical ; Materials Science, Multidisciplinary
项目资助者National Key Natural Science Foundation of China(21136009) ; National Natural Science Foundation of China(21106162)
WOS记录号WOS:000369457900020
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20606
专题生化工程国家重点实验室
作者单位1.Xiangtan Univ, Coll Chem Engn, Xiangtan 411105, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
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GB/T 7714
Liu, Zhini,Yang, Liangrong,Dong, Tingting,et al. Gas-assisted magnetic separation for the purification of proteins in batch systems[J]. PARTICUOLOGY,2016,24(FEB):170-176.
APA Liu, Zhini.,Yang, Liangrong.,Dong, Tingting.,Li, Wensong.,Sun, Xitong.,...&Liu, Huizhou.(2016).Gas-assisted magnetic separation for the purification of proteins in batch systems.PARTICUOLOGY,24(FEB),170-176.
MLA Liu, Zhini,et al."Gas-assisted magnetic separation for the purification of proteins in batch systems".PARTICUOLOGY 24.FEB(2016):170-176.
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