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Gas-assisted magnetic separation for the purification of proteins in batch systems
Liu, Zhini1,2; Yang, Liangrong2; Dong, Tingting2; Li, Wensong1,2; Sun, Xitong2; Zhu, Menghao2; Duan, Zhengkang1; Liu, Qingfen2; Liu, Huizhou2
Source PublicationPARTICUOLOGY
AbstractIn this paper, gas-assisted magnetic separation (GAMS), a technique that combines magnetic separation with flotation, was investigated for the potential large-scale separation of proteins. The GAMS process includes adsorption of target proteins and magnetic separation to recover protein-loaded magnetic particles from the dilute biosuspension with the assistance of bubbles. Microsized ethylenediamine-functionalized poly(glycidyl methacrylate) superparamagnetic microspheres (MPMs) and bovine serum albumin (BSA) were used as a model system. The feasibility of GAMS for capturing BSA-loaded MPMs from an appropriate medium was shown. High recovery of BSA-loaded MPMs was obtained by simple adjustment of the initial solution pH without extra detergents and antifoaming agents. The GAMS conditions were consistent with the adsorption conditions, and no proteins were desorbed from the MPMs during this process. Under the optimal conditions, the separation rate and recovery percentage reached 410 mL/min and 98% in 0.61 min, respectively. Conformational changes of BSA during the GAMS process were investigated by fluorescence spectroscopy and circular dichroism spectrometry. (C) 2015 Chinese Society of Particuology and Institute of Process Engineering, Chinese Academy of Sciences. Published by Elsevier B.V. All rights reserved.
KeywordAdsorption Magnetic Separation Flotation Recovery Bsa Conformation
WOS HeadingsScience & Technology ; Technology
Indexed BySCI
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Chemical ; Materials Science, Multidisciplinary
Funding OrganizationNational Key Natural Science Foundation of China(21136009) ; National Natural Science Foundation of China(21106162)
WOS IDWOS:000369457900020
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Xiangtan Univ, Coll Chem Engn, Xiangtan 411105, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Liu, Zhini,Yang, Liangrong,Dong, Tingting,et al. Gas-assisted magnetic separation for the purification of proteins in batch systems[J]. PARTICUOLOGY,2016,24(FEB):170-176.
APA Liu, Zhini.,Yang, Liangrong.,Dong, Tingting.,Li, Wensong.,Sun, Xitong.,...&Liu, Huizhou.(2016).Gas-assisted magnetic separation for the purification of proteins in batch systems.PARTICUOLOGY,24(FEB),170-176.
MLA Liu, Zhini,et al."Gas-assisted magnetic separation for the purification of proteins in batch systems".PARTICUOLOGY 24.FEB(2016):170-176.
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