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Expression and Characterization of a New Thermostable Esterase from Clostridium thermocellum | |
Zhang, TX; Chen, HY; Ni, Z; Tian, R; Jia, JR; Chen, Z; Yang, SL | |
2015 | |
Source Publication | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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ISSN | 0273-2289 |
Volume | 177Issue:7Pages:1437 |
Abstract | The thermostable esterase from the thermophilic bacterium Clostridium thermocellum DSM 1313 was expressed in Escherichia coli and purified by Ni2+ affinity chromatography. Its molecular weight was approximately 35 kDa according to 12 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The enzyme exhibited the highest specific activity with p-nitrophenyl butyrate (285 s(-1) mM(-1)). The activity of the esterase was greatest at 65 A degrees C, and the esterase maintained residual activity levels of 70 and 50 % after 3 h incubation at 65 and 70 A degrees C, respectively. Its activity was optimal at pH 7.0, was enhanced in the presence of Ca2+ and Mg2+, and was inhibited by Ni2+ and Cu2+. The addition of surfactants, such as Tween-20, Tween-80, Triton X-100, and SDS, at concentrations of 5 % (v/v) significantly inhibited the lipolytic action of the esterase. Enzyme activity was relatively stable in 10 % methanol, and 50 % residual activity was seen in 10 % DMSO, demonstrating its potential in biodiesel production and industrial applications. |
Keyword | Thermostable Thermotoga-maritima Esterase Enzymes Expression Bacterium Characterization Cloning Clostridium Thermocellum Family Site |
Subtype | Article |
DOI | 10.1007/s12010-015-1824-7 |
Indexed By | SCI |
Language | 英语 |
WOS ID | WOS:000365165500004 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ipe.ac.cn/handle/122111/20626 |
Collection | 研究所(批量导入) |
Corresponding Author | Chen, HY |
Recommended Citation GB/T 7714 | Zhang, TX,Chen, HY,Ni, Z,et al. Expression and Characterization of a New Thermostable Esterase from Clostridium thermocellum[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2015,177(7):1437. |
APA | Zhang, TX.,Chen, HY.,Ni, Z.,Tian, R.,Jia, JR.,...&Yang, SL.(2015).Expression and Characterization of a New Thermostable Esterase from Clostridium thermocellum.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,177(7),1437. |
MLA | Zhang, TX,et al."Expression and Characterization of a New Thermostable Esterase from Clostridium thermocellum".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 177.7(2015):1437. |
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Expression and Chara(760KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text |
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