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Expression and Characterization of a New Thermostable Esterase from Clostridium thermocellum
Zhang, TX; Chen, HY; Ni, Z; Tian, R; Jia, JR; Chen, Z; Yang, SL
2015
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
Volume177Issue:7Pages:1437
Abstract

The thermostable esterase from the thermophilic bacterium Clostridium thermocellum DSM 1313 was expressed in Escherichia coli and purified by Ni2+ affinity chromatography. Its molecular weight was approximately 35 kDa according to 12 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The enzyme exhibited the highest specific activity with p-nitrophenyl butyrate (285 s(-1) mM(-1)). The activity of the esterase was greatest at 65 A degrees C, and the esterase maintained residual activity levels of 70 and 50 % after 3 h incubation at 65 and 70 A degrees C, respectively. Its activity was optimal at pH 7.0, was enhanced in the presence of Ca2+ and Mg2+, and was inhibited by Ni2+ and Cu2+. The addition of surfactants, such as Tween-20, Tween-80, Triton X-100, and SDS, at concentrations of 5 % (v/v) significantly inhibited the lipolytic action of the esterase. Enzyme activity was relatively stable in 10 % methanol, and 50 % residual activity was seen in 10 % DMSO, demonstrating its potential in biodiesel production and industrial applications.

KeywordThermostable Thermotoga-maritima Esterase Enzymes Expression Bacterium Characterization Cloning Clostridium Thermocellum Family Site
SubtypeArticle
DOI10.1007/s12010-015-1824-7
Indexed BySCI
Language英语
WOS IDWOS:000365165500004
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/20626
Collection研究所(批量导入)
Corresponding AuthorChen, HY
Recommended Citation
GB/T 7714
Zhang, TX,Chen, HY,Ni, Z,et al. Expression and Characterization of a New Thermostable Esterase from Clostridium thermocellum[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2015,177(7):1437.
APA Zhang, TX.,Chen, HY.,Ni, Z.,Tian, R.,Jia, JR.,...&Yang, SL.(2015).Expression and Characterization of a New Thermostable Esterase from Clostridium thermocellum.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,177(7),1437.
MLA Zhang, TX,et al."Expression and Characterization of a New Thermostable Esterase from Clostridium thermocellum".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 177.7(2015):1437.
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