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Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration
Chen, Guoqiang1,2; Song, Weijie1; Qi, Benkun1; Ghosh, Raja3; Wan, Yinhua1
2016-05-15
发表期刊BIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
卷号109期号:MAY页码:127-136
摘要

For the purpose of residual PEG removal after protein purification and PEGylation, electro-ultrafiltration (EUF) was used for separation of human serum albumin (HSA) from polyethylene glycol (PEG) with different molecular weights. The results clearly demonstrated the effectiveness of EUF for achieving the desired objectives. Higher flux and lower HSA transmission were observed in EUF process with the feed consisting of a mixture of 1 g/L PEG and 1 g/L HSA, compared to conventional ultrafiltration (UF) process, while the PEG transmission was nearly the same. When the concentration of PEG increased from 1 g/L to 10 g/L while keeping the HSA concentration the same, more HSA passed through the membrane in the UF process due to reduction in protein diffusion and hydration by PEG. When the HSA concentration increased from 1 g/L to 10 g/L with the PEG concentration being 1 g/L, the PEG transmission decreased significantly. The application of electric field resulted in high flux and PEG transmission and low protein transmission through the membrane. The effect of electrolyte (i.e., ionic strength and type) on the efficiency of the EUF process was also investigated. (C) 2016 Elsevier B.V. All rights reserved.

关键词Ultrafiltration Electro-ultrafiltration Human Serum Albumin Polyethylene Glycol Concentration Polarization
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.bej.2016.01.004
收录类别SCI
语种英语
关键词[WOS]Cross-flow Ultrafiltration ; Aqueous 2-phase Systems ; Electric-field ; Poly(Ethylene Glycol) ; Fruit Juice ; Proteins ; Membrane ; Removal ; Electrophoresis ; Retention
WOS研究方向Biotechnology & Applied Microbiology ; Engineering
WOS类目Biotechnology & Applied Microbiology ; Engineering, Chemical
项目资助者National Natural Science Foundation of China(20976175) ; Chinese Academy of Sciences Visiting Professorship for Senior International Scientists(2010T2G27)
WOS记录号WOS:000372387200016
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20628
专题研究所(批量导入)
通讯作者Wan, Yinhua
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.McMaster Univ, Dept Chem Engn, Hamilton, ON L8S 4L8, Canada
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GB/T 7714
Chen, Guoqiang,Song, Weijie,Qi, Benkun,et al. Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration[J]. BIOCHEMICAL ENGINEERING JOURNAL,2016,109(MAY):127-136.
APA Chen, Guoqiang,Song, Weijie,Qi, Benkun,Ghosh, Raja,&Wan, Yinhua.(2016).Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration.BIOCHEMICAL ENGINEERING JOURNAL,109(MAY),127-136.
MLA Chen, Guoqiang,et al."Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration".BIOCHEMICAL ENGINEERING JOURNAL 109.MAY(2016):127-136.
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