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Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration
Chen, Guoqiang1,2; Song, Weijie1; Qi, Benkun1; Ghosh, Raja3; Wan, Yinhua1

For the purpose of residual PEG removal after protein purification and PEGylation, electro-ultrafiltration (EUF) was used for separation of human serum albumin (HSA) from polyethylene glycol (PEG) with different molecular weights. The results clearly demonstrated the effectiveness of EUF for achieving the desired objectives. Higher flux and lower HSA transmission were observed in EUF process with the feed consisting of a mixture of 1 g/L PEG and 1 g/L HSA, compared to conventional ultrafiltration (UF) process, while the PEG transmission was nearly the same. When the concentration of PEG increased from 1 g/L to 10 g/L while keeping the HSA concentration the same, more HSA passed through the membrane in the UF process due to reduction in protein diffusion and hydration by PEG. When the HSA concentration increased from 1 g/L to 10 g/L with the PEG concentration being 1 g/L, the PEG transmission decreased significantly. The application of electric field resulted in high flux and PEG transmission and low protein transmission through the membrane. The effect of electrolyte (i.e., ionic strength and type) on the efficiency of the EUF process was also investigated. (C) 2016 Elsevier B.V. All rights reserved.

KeywordUltrafiltration Electro-ultrafiltration Human Serum Albumin Polyethylene Glycol Concentration Polarization
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
Indexed BySCI
WOS KeywordCross-flow Ultrafiltration ; Aqueous 2-phase Systems ; Electric-field ; Poly(Ethylene Glycol) ; Fruit Juice ; Proteins ; Membrane ; Removal ; Electrophoresis ; Retention
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNational Natural Science Foundation of China(20976175) ; Chinese Academy of Sciences Visiting Professorship for Senior International Scientists(2010T2G27)
WOS IDWOS:000372387200016
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorWan, Yinhua
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.McMaster Univ, Dept Chem Engn, Hamilton, ON L8S 4L8, Canada
Recommended Citation
GB/T 7714
Chen, Guoqiang,Song, Weijie,Qi, Benkun,et al. Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration[J]. BIOCHEMICAL ENGINEERING JOURNAL,2016,109(MAY):127-136.
APA Chen, Guoqiang,Song, Weijie,Qi, Benkun,Ghosh, Raja,&Wan, Yinhua.(2016).Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration.BIOCHEMICAL ENGINEERING JOURNAL,109(MAY),127-136.
MLA Chen, Guoqiang,et al."Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration".BIOCHEMICAL ENGINEERING JOURNAL 109.MAY(2016):127-136.
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