CAS OpenIR  > 研究所(批量导入)
Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration
Chen, Guoqiang1,2; Song, Weijie1; Qi, Benkun1; Ghosh, Raja3; Wan, Yinhua1
2016-05-15
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume109Issue:MAYPages:127-136
Abstract

For the purpose of residual PEG removal after protein purification and PEGylation, electro-ultrafiltration (EUF) was used for separation of human serum albumin (HSA) from polyethylene glycol (PEG) with different molecular weights. The results clearly demonstrated the effectiveness of EUF for achieving the desired objectives. Higher flux and lower HSA transmission were observed in EUF process with the feed consisting of a mixture of 1 g/L PEG and 1 g/L HSA, compared to conventional ultrafiltration (UF) process, while the PEG transmission was nearly the same. When the concentration of PEG increased from 1 g/L to 10 g/L while keeping the HSA concentration the same, more HSA passed through the membrane in the UF process due to reduction in protein diffusion and hydration by PEG. When the HSA concentration increased from 1 g/L to 10 g/L with the PEG concentration being 1 g/L, the PEG transmission decreased significantly. The application of electric field resulted in high flux and PEG transmission and low protein transmission through the membrane. The effect of electrolyte (i.e., ionic strength and type) on the efficiency of the EUF process was also investigated. (C) 2016 Elsevier B.V. All rights reserved.

KeywordUltrafiltration Electro-ultrafiltration Human Serum Albumin Polyethylene Glycol Concentration Polarization
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.bej.2016.01.004
Indexed BySCI
Language英语
WOS KeywordCross-flow Ultrafiltration ; Aqueous 2-phase Systems ; Electric-field ; Poly(Ethylene Glycol) ; Fruit Juice ; Proteins ; Membrane ; Removal ; Electrophoresis ; Retention
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNational Natural Science Foundation of China(20976175) ; Chinese Academy of Sciences Visiting Professorship for Senior International Scientists(2010T2G27)
WOS IDWOS:000372387200016
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/20628
Collection研究所(批量导入)
Corresponding AuthorWan, Yinhua
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.McMaster Univ, Dept Chem Engn, Hamilton, ON L8S 4L8, Canada
Recommended Citation
GB/T 7714
Chen, Guoqiang,Song, Weijie,Qi, Benkun,et al. Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration[J]. BIOCHEMICAL ENGINEERING JOURNAL,2016,109(MAY):127-136.
APA Chen, Guoqiang,Song, Weijie,Qi, Benkun,Ghosh, Raja,&Wan, Yinhua.(2016).Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration.BIOCHEMICAL ENGINEERING JOURNAL,109(MAY),127-136.
MLA Chen, Guoqiang,et al."Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration".BIOCHEMICAL ENGINEERING JOURNAL 109.MAY(2016):127-136.
Files in This Item:
File Name/Size DocType Version Access License
Separation of human (2985KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, Guoqiang]'s Articles
[Song, Weijie]'s Articles
[Qi, Benkun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, Guoqiang]'s Articles
[Song, Weijie]'s Articles
[Qi, Benkun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, Guoqiang]'s Articles
[Song, Weijie]'s Articles
[Qi, Benkun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.