Knowledge Management System Of Institute of process engineering,CAS
Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration | |
Chen, Guoqiang1,2; Song, Weijie1; Qi, Benkun1; Ghosh, Raja3; Wan, Yinhua1 | |
2016-05-15 | |
Source Publication | BIOCHEMICAL ENGINEERING JOURNAL
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ISSN | 1369-703X |
Volume | 109Issue:MAYPages:127-136 |
Abstract | For the purpose of residual PEG removal after protein purification and PEGylation, electro-ultrafiltration (EUF) was used for separation of human serum albumin (HSA) from polyethylene glycol (PEG) with different molecular weights. The results clearly demonstrated the effectiveness of EUF for achieving the desired objectives. Higher flux and lower HSA transmission were observed in EUF process with the feed consisting of a mixture of 1 g/L PEG and 1 g/L HSA, compared to conventional ultrafiltration (UF) process, while the PEG transmission was nearly the same. When the concentration of PEG increased from 1 g/L to 10 g/L while keeping the HSA concentration the same, more HSA passed through the membrane in the UF process due to reduction in protein diffusion and hydration by PEG. When the HSA concentration increased from 1 g/L to 10 g/L with the PEG concentration being 1 g/L, the PEG transmission decreased significantly. The application of electric field resulted in high flux and PEG transmission and low protein transmission through the membrane. The effect of electrolyte (i.e., ionic strength and type) on the efficiency of the EUF process was also investigated. (C) 2016 Elsevier B.V. All rights reserved. |
Keyword | Ultrafiltration Electro-ultrafiltration Human Serum Albumin Polyethylene Glycol Concentration Polarization |
Subtype | Article |
WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Technology |
DOI | 10.1016/j.bej.2016.01.004 |
Indexed By | SCI |
Language | 英语 |
WOS Keyword | Cross-flow Ultrafiltration ; Aqueous 2-phase Systems ; Electric-field ; Poly(Ethylene Glycol) ; Fruit Juice ; Proteins ; Membrane ; Removal ; Electrophoresis ; Retention |
WOS Research Area | Biotechnology & Applied Microbiology ; Engineering |
WOS Subject | Biotechnology & Applied Microbiology ; Engineering, Chemical |
Funding Organization | National Natural Science Foundation of China(20976175) ; Chinese Academy of Sciences Visiting Professorship for Senior International Scientists(2010T2G27) |
WOS ID | WOS:000372387200016 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ipe.ac.cn/handle/122111/20628 |
Collection | 研究所(批量导入) |
Corresponding Author | Wan, Yinhua |
Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.McMaster Univ, Dept Chem Engn, Hamilton, ON L8S 4L8, Canada |
Recommended Citation GB/T 7714 | Chen, Guoqiang,Song, Weijie,Qi, Benkun,et al. Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration[J]. BIOCHEMICAL ENGINEERING JOURNAL,2016,109(MAY):127-136. |
APA | Chen, Guoqiang,Song, Weijie,Qi, Benkun,Ghosh, Raja,&Wan, Yinhua.(2016).Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration.BIOCHEMICAL ENGINEERING JOURNAL,109(MAY),127-136. |
MLA | Chen, Guoqiang,et al."Separation of human serum albumin and polyethylene glycol by electro-ultrafiltration".BIOCHEMICAL ENGINEERING JOURNAL 109.MAY(2016):127-136. |
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Separation of human (2985KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text |
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