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Enhanced non-inflammasome mediated immune responses by mannosylated zwitterionic-based cationic liposomes for HIV DNA vaccines
Qiao, Chenmeng1,2; Liu, Jiandong3; Yang, Jun1; Li, Yan1; Weng, Jie2; Shao, Yiming3; Zhang, Xin1
2016-04-01
发表期刊BIOMATERIALS
ISSN0142-9612
卷号85期号:APR页码:1-17
摘要

Human immunodeficiency virus (HIV) DNA vaccine can induce cellular and humoral immunity. A safe and effective HIV DNA vaccine is urgent need to prevent the spread of acquired immune deficiency syndrome (AIDS). The major drawback of DNA vaccines is the low immunogenicity, which is caused by the poor delivery to antigen presenting cells and insufficient antigen expression. Sparked by the capability of endosomal/lysosomal escape of the zwitterionic lipid distearoyl phosphoethanol-amine-polycarboxybetaine (DSPE-PCB), we attempted to develop a zwitterionic-based cationic liposome with enhanced immunogenicity of DNA vaccines. The mannosylated zwitterionic-based cationic liposome (man-ZCL) was constructed as a DNA vaccine adjuvant for HIV vaccination. Man-ZCL could complex with DNA antigens to form a tight structure and protect them from nuclei enzyme degradation. Benefited from the capability of the specific mannose receptor mediated antigen processing cells targeting and enhanced endosomal/lysosomal escape, the man-ZCL lipoplexes were supposed to promote antigen presentation and the immunogenicity of DNA vaccines. In vitro and in vivo results revealed that man-ZCL lipoplexes showed enhanced anti-HIV immune responses and lower toxicity compared with CpG/DNA and Lipo2k/DNA, and triggered a Th1/Th2 mixed immunity. An antigen-depot effect was observed in the administration site, and this resulted in enhanced retention of DNA antigens in draining lymph nodes. Most importantly, the man-ZCL could assist to activate T cells through a non-inflammasome pathway. These findings suggested that the man-ZCL could be potentially applied as a safe and efficient DNA adjuvant for HIV vaccines. (C) 2016 Elsevier Ltd. All rights reserved.

关键词Mannosylated Zwitterionic-based Cationic Liposome Hiv Dna Vaccine Adjuvant Non-inflammasome Pathway Immune Response
文章类型Article
WOS标题词Science & Technology ; Technology
DOI10.1016/j.biomaterials.2016.01.054
收录类别SCI
语种英语
关键词[WOS]Layered Double Hydroxides ; Gene Delivery ; Poly(Beta-amino Esters) ; Dendritic Cells ; Plasmid Dna ; In-vivo ; Nanoparticles ; Vaccination ; Adjuvant ; Immunogenicity
WOS研究方向Engineering ; Materials Science
WOS类目Engineering, Biomedical ; Materials Science, bioMaterials
项目资助者National Natural Science Foundation of China(21304099 ; National High Technology Research and Development Program(2014AA020708)
WOS记录号WOS:000371841400001
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20629
专题研究所(批量导入)
通讯作者Zhang, Xin
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Southwest Jiaotong Univ, Sch Mat Sci & Engn, Key Lab Adv Technol Mat, Minist Educ, Chengdu 610031, Peoples R China
3.Chinese Ctr Dis Control & Prevent, Collaborat Innovat Ctr Diag & Treatment Infect Di, Natl Ctr AIDS STD Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China
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Qiao, Chenmeng,Liu, Jiandong,Yang, Jun,et al. Enhanced non-inflammasome mediated immune responses by mannosylated zwitterionic-based cationic liposomes for HIV DNA vaccines[J]. BIOMATERIALS,2016,85(APR):1-17.
APA Qiao, Chenmeng.,Liu, Jiandong.,Yang, Jun.,Li, Yan.,Weng, Jie.,...&Zhang, Xin.(2016).Enhanced non-inflammasome mediated immune responses by mannosylated zwitterionic-based cationic liposomes for HIV DNA vaccines.BIOMATERIALS,85(APR),1-17.
MLA Qiao, Chenmeng,et al."Enhanced non-inflammasome mediated immune responses by mannosylated zwitterionic-based cationic liposomes for HIV DNA vaccines".BIOMATERIALS 85.APR(2016):1-17.
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