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High-throughput screening of high Monascus pigment-producing strain based on digital image processing
Xia, Meng-lei1,2; Wang, Lan1; Yang, Zhi-xia3; Chen, Hong-zhang1
2016-04-01
发表期刊JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
ISSN1367-5435
卷号43期号:4页码:451-461
摘要

This work proposed a new method which applied image processing and support vector machine (SVM) for screening of mold strains. Taking Monascus as example, morphological characteristics of Monascus colony were quantified by image processing. And the association between the characteristics and pigment production capability was determined by SVM. On this basis, a highly automated screening strategy was achieved. The accuracy of the proposed strategy is 80.6 %, which is compatible with the existing methods (81.1 % for microplate and 85.4 % for flask). Meanwhile, the screening of 500 colonies only takes 20-30 min, which is the highest rate among all published results. By applying this automated method, 13 strains with high-predicted production were obtained and the best one produced as 2.8-fold (226 U/mL) of pigment and 1.9-fold (51 mg/L) of lovastatin compared with the parent strain. The current study provides us with an effective and promising method for strain improvement.

关键词Monascus Ruber Pigment Image Processing Support Vector Machine Automatized Operation
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10295-015-1729-z
URL查看原文
收录类别SCI
语种英语
关键词[WOS]Support Vector Machine ; Submerged Fermentation ; Morphology ; Aspergillus ; Purpureus ; Culture
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
项目资助者National Key Laboratory of Biochemical Engineering(2013KF-01) ; National High Technology Research and Development Program (863 Program)(2012AA021302)
WOS记录号WOS:000372538900003
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20651
专题研究所(批量导入)
通讯作者Chen, Hong-zhang
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Xinjiang Univ, Coll Math & Syst Sci, Urumqi 830046, Peoples R China
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Xia, Meng-lei,Wang, Lan,Yang, Zhi-xia,et al. High-throughput screening of high Monascus pigment-producing strain based on digital image processing[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2016,43(4):451-461.
APA Xia, Meng-lei,Wang, Lan,Yang, Zhi-xia,&Chen, Hong-zhang.(2016).High-throughput screening of high Monascus pigment-producing strain based on digital image processing.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,43(4),451-461.
MLA Xia, Meng-lei,et al."High-throughput screening of high Monascus pigment-producing strain based on digital image processing".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 43.4(2016):451-461.
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