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High molecular weight beta-poly(L-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture | |
Cao, Weifeng1; Chen, Xiangrong1; Luo, Jianquan1; Yin, Junxiang2; Qiao, Changsheng3; Wan, Yinhua1 | |
2016-04-01 | |
Source Publication | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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ISSN | 0141-8130 |
Volume | 85Issue:APRPages:192-199 |
Abstract | beta-Poly(malic acid) (PMLA) has attracted increasing attentions because of its potential application in medicine and other industries. In this study, the variation of PMLA molecular weight (M-w) in the batch culture and the strategies to enhance PMLA M-w were studied. Adding exogenous Ca2+ (0.1 g/L CaCl2) to the medium caused a significant increase in both PMLA concentration and M-w (11.38% and 26.3%, respectively) when Na2CO3 was used as the neutralizer. The M-w of PMLA during the process of batch culture, which associated with the specific PMLA production per unit cell mass (Y-p/x) before glucose was depleted, increased from 12.522KDa to its maximum 18.693KDa and then kept decreasing until the end of the culture. Compared with the results in batch culture, M-w increased by 84.4% (up to 19.51 kDa) with a productivity of 1.1 gh(-1) L-1 when the cells were maintained in exponential growth phase during Ca2+ added repeated batch culture. The present work provides an efficient approach to obtain superior quality PMLA product with high M-w. (C) 2015 Elsevier B.V. All rights reserved. |
Keyword | Beta-poly(L-malic Acid) Aureobasidium Pullulans Molecular Weight |
Subtype | Article |
Subject Area | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science ; Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1016/j.ijbiomac.2015.12.056 |
URL | 查看原文 |
Indexed By | SCI |
Language | 英语 |
WOS Keyword | Fungus Aureobasidium-pullulans ; Poly-l-malate ; L-malic Acid ; Physarum-polycephalum ; Polymalic Acid ; Saccharomyces-cerevisiae ; Melanin ; Strain ; Fermentation ; Combination |
WOS Research Area | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
Funding Organization | National Science Foundation of China(21406240) ; Chinese Academy of Sciences' Key Deployment Project(KSZD-EW-2-017-3) ; National High Technology Research and Development Program of China(2014AA021005 ; 2015AA021002) |
WOS ID | WOS:000370886800022 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ipe.ac.cn/handle/122111/20692 |
Collection | 研究所(批量导入) |
Corresponding Author | Wan, Yinhua |
Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.China Natl Ctr Biotechnol Dev, Beijing 100036, Peoples R China 3.Tianjin Univ Sci & Technol, Dept Bioengn, Tianjin 300457, Peoples R China |
Recommended Citation GB/T 7714 | Cao, Weifeng,Chen, Xiangrong,Luo, Jianquan,et al. High molecular weight beta-poly(L-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,85(APR):192-199. |
APA | Cao, Weifeng,Chen, Xiangrong,Luo, Jianquan,Yin, Junxiang,Qiao, Changsheng,&Wan, Yinhua.(2016).High molecular weight beta-poly(L-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,85(APR),192-199. |
MLA | Cao, Weifeng,et al."High molecular weight beta-poly(L-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 85.APR(2016):192-199. |
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High molecular weigh(1960KB) | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text |
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