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High molecular weight beta-poly(L-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture
Cao, Weifeng1; Chen, Xiangrong1; Luo, Jianquan1; Yin, Junxiang2; Qiao, Changsheng3; Wan, Yinhua1
2016-04-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume85Issue:APRPages:192-199
Abstract

beta-Poly(malic acid) (PMLA) has attracted increasing attentions because of its potential application in medicine and other industries. In this study, the variation of PMLA molecular weight (M-w) in the batch culture and the strategies to enhance PMLA M-w were studied. Adding exogenous Ca2+ (0.1 g/L CaCl2) to the medium caused a significant increase in both PMLA concentration and M-w (11.38% and 26.3%, respectively) when Na2CO3 was used as the neutralizer. The M-w of PMLA during the process of batch culture, which associated with the specific PMLA production per unit cell mass (Y-p/x) before glucose was depleted, increased from 12.522KDa to its maximum 18.693KDa and then kept decreasing until the end of the culture. Compared with the results in batch culture, M-w increased by 84.4% (up to 19.51 kDa) with a productivity of 1.1 gh(-1) L-1 when the cells were maintained in exponential growth phase during Ca2+ added repeated batch culture. The present work provides an efficient approach to obtain superior quality PMLA product with high M-w. (C) 2015 Elsevier B.V. All rights reserved.

KeywordBeta-poly(L-malic Acid) Aureobasidium Pullulans Molecular Weight
SubtypeArticle
Subject AreaBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science ; Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.ijbiomac.2015.12.056
URL查看原文
Indexed BySCI
Language英语
WOS KeywordFungus Aureobasidium-pullulans ; Poly-l-malate ; L-malic Acid ; Physarum-polycephalum ; Polymalic Acid ; Saccharomyces-cerevisiae ; Melanin ; Strain ; Fermentation ; Combination
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
Funding OrganizationNational Science Foundation of China(21406240) ; Chinese Academy of Sciences' Key Deployment Project(KSZD-EW-2-017-3) ; National High Technology Research and Development Program of China(2014AA021005 ; 2015AA021002)
WOS IDWOS:000370886800022
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/20692
Collection研究所(批量导入)
Corresponding AuthorWan, Yinhua
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.China Natl Ctr Biotechnol Dev, Beijing 100036, Peoples R China
3.Tianjin Univ Sci & Technol, Dept Bioengn, Tianjin 300457, Peoples R China
Recommended Citation
GB/T 7714
Cao, Weifeng,Chen, Xiangrong,Luo, Jianquan,et al. High molecular weight beta-poly(L-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2016,85(APR):192-199.
APA Cao, Weifeng,Chen, Xiangrong,Luo, Jianquan,Yin, Junxiang,Qiao, Changsheng,&Wan, Yinhua.(2016).High molecular weight beta-poly(L-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,85(APR),192-199.
MLA Cao, Weifeng,et al."High molecular weight beta-poly(L-malic acid) produced by A. pullulans with Ca2+ added repeated batch culture".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 85.APR(2016):192-199.
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