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A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane
Zhang, D; Zhao, H; Fan, ZJ; Li, MJ; Du, PH; Liu, CM; Li, YP; Li, HT; Cao, HB
2015
发表期刊PLOS ONE
ISSN1932-6203
卷号10期号:6页码:e0130156
摘要

A sensitive and noble amperometric horseradish peroxidase (HRP) biosensor is fabricated via the deposition of gold nanoparticles (AuNPs) onto a three-dimensional (3D) porous carbonized chicken eggshell membrane (CESM). Due to the synergistic effects of the unique porous carbon architecture and well-distributed AuNPs, the enzyme-modified electrode shows an excellent electrochemical redox behavior. Compared with bare glass carbon electrode (GCE), the cathodic peak current of the enzymatic electrode increases 12.6 times at a formal potential of -100mV (vs. SCE) and charge-transfer resistance decreases 62.8%. Additionally, the AuNPs-CESM electrode exhibits a good biocompatibility, which effectively retains its bioactivity with a surface coverage of HRP 6.39x10(-9)mol cm(-2) (752 times higher than the theoretical monolayer coverage of HRP). Furthermore, the HRP-AuNPs-CESM-GCE electrode, as a biosensor for H2O2 detection, has a good accuracy and high sensitivity with the linear range of 0.01-2.7 mM H2O2 and the detection limit of 3 mu M H2O2(S/N = 3).

关键词Direct Electron-transfer Horseradish-peroxidase Electrochemical Biosensor Functionalized-graphene Biomass Carbon Nanotube Immobilization Thionine Mediator Hrp
文章类型Article
学科领域Multidisciplinary Sciences ; Science & Technology - Other Topics
DOI10.1371/journal.pone.0130156
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000356327000144
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20699
专题研究所(批量导入)
通讯作者Zhao, H
推荐引用方式
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Zhang, D,Zhao, H,Fan, ZJ,et al. A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane[J]. PLOS ONE,2015,10(6):e0130156.
APA Zhang, D.,Zhao, H.,Fan, ZJ.,Li, MJ.,Du, PH.,...&Cao, HB.(2015).A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane.PLOS ONE,10(6),e0130156.
MLA Zhang, D,et al."A Highly Sensitive and Selective Hydrogen Peroxide Biosensor Based on Gold Nanoparticles and Three-Dimensional Porous Carbonized Chicken Eggshell Membrane".PLOS ONE 10.6(2015):e0130156.
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