| Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli | |
| Olajuyin, Ayobami Matthew1,2; Yang, Maohua1; Liu, Yilan1; Mu, Tingzhen1,2; Tian, Jiangnan1,2; Adaramoye, Oluwatosin Adekunle3; Xing, Jianmin1 | |
| 2016-08-01 | |
| Source Publication | BIORESOURCE TECHNOLOGY
 |
| ISSN | 0960-8524 |
| Volume | 214Issue:AUGPages:653-659 |
| Abstract | Succinic acid, a C4 dicarboxylic acid is used in many fields such as food, agriculture, pharmaceutical and polymer industries. In this study, microbial production of succinic acid from Palmaria palmata was investigated for the first time. In engineered Escherichia coli KLPPP, lactate dehydrogenase, pyruvate formate lyase, phosphotransacetylase-acetate kinase and pyruvate oxidase genes were deleted while phosphoenolpyruvate carboxykinase was overexpressed. The recombinant exhibited higher molar yield of succinic acid on galactose (1.20 +/- 0.02 mol/mol) than glucose (0.48 +/- 0.03 mol/mol). The concentration and molar yield of succinic acid were 22.40 +/- 0.12 g/L and 1.13 +/- 0.02 mol/mol total sugar respectively after 72 h dual phase fermentation from P. palmata hydrolysate which composed of glucose (12.57 +/- 0.17 g/L) and galactose (18.03 +/- 0.10 g/L). The results demonstrate that P. palmata red macroalgae biomass represents a novel and an economically alternative feedstock for biochemicals production. (C) 2016 Elsevier Ltd. All rights reserved. |
| Keyword | Palmaria Palmata Escherichia Coli Succinic Acid Galactose Fermentation |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| DOI | 10.1016/j.biortech.2016.04.117 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | ANAEROBIOSPIRILLUM-SUCCINICIPRODUCENS ; BIOFUEL PRODUCTION ; DIAUXIC GROWTH ; FERMENTATION ; MACROALGAE ; GALACTOSE ; GLUCOSE ; STRAIN ; PATHWAYS ; MUTATION |
| WOS Research Area | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS Subject | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| Funding Organization | National High Technology Research and Development Program of China (863 Project)(2014AA021905) |
| WOS ID | WOS:000377366900083 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/20957 |
| Collection | 生化工程国家重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Ibadan, Dept Biochem, Fac Basic Med Sci, Ibadan, Nigeria |
| Recommended Citation GB/T 7714 | Olajuyin, Ayobami Matthew,Yang, Maohua,Liu, Yilan,et al. Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli[J]. BIORESOURCE TECHNOLOGY,2016,214(AUG):653-659. |
| APA | Olajuyin, Ayobami Matthew.,Yang, Maohua.,Liu, Yilan.,Mu, Tingzhen.,Tian, Jiangnan.,...&Xing, Jianmin.(2016).Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli.BIORESOURCE TECHNOLOGY,214(AUG),653-659. |
| MLA | Olajuyin, Ayobami Matthew,et al."Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli".BIORESOURCE TECHNOLOGY 214.AUG(2016):653-659. |
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| File Name/Size | DocType | Version | Access | License | ||
| Efficient production（582KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text | |
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