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Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli
Olajuyin, Ayobami Matthew1,2; Yang, Maohua1; Liu, Yilan1; Mu, Tingzhen1,2; Tian, Jiangnan1,2; Adaramoye, Oluwatosin Adekunle3; Xing, Jianmin1
2016-08-01
发表期刊BIORESOURCE TECHNOLOGY
ISSN0960-8524
卷号214期号:AUG页码:653-659
摘要Succinic acid, a C4 dicarboxylic acid is used in many fields such as food, agriculture, pharmaceutical and polymer industries. In this study, microbial production of succinic acid from Palmaria palmata was investigated for the first time. In engineered Escherichia coli KLPPP, lactate dehydrogenase, pyruvate formate lyase, phosphotransacetylase-acetate kinase and pyruvate oxidase genes were deleted while phosphoenolpyruvate carboxykinase was overexpressed. The recombinant exhibited higher molar yield of succinic acid on galactose (1.20 +/- 0.02 mol/mol) than glucose (0.48 +/- 0.03 mol/mol). The concentration and molar yield of succinic acid were 22.40 +/- 0.12 g/L and 1.13 +/- 0.02 mol/mol total sugar respectively after 72 h dual phase fermentation from P. palmata hydrolysate which composed of glucose (12.57 +/- 0.17 g/L) and galactose (18.03 +/- 0.10 g/L). The results demonstrate that P. palmata red macroalgae biomass represents a novel and an economically alternative feedstock for biochemicals production. (C) 2016 Elsevier Ltd. All rights reserved.
关键词Palmaria Palmata Escherichia Coli Succinic Acid Galactose Fermentation
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2016.04.117
收录类别SCI
语种英语
关键词[WOS]ANAEROBIOSPIRILLUM-SUCCINICIPRODUCENS ; BIOFUEL PRODUCTION ; DIAUXIC GROWTH ; FERMENTATION ; MACROALGAE ; GALACTOSE ; GLUCOSE ; STRAIN ; PATHWAYS ; MUTATION
WOS研究方向Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
项目资助者National High Technology Research and Development Program of China (863 Project)(2014AA021905)
WOS记录号WOS:000377366900083
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20957
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Ibadan, Dept Biochem, Fac Basic Med Sci, Ibadan, Nigeria
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Olajuyin, Ayobami Matthew,Yang, Maohua,Liu, Yilan,et al. Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli[J]. BIORESOURCE TECHNOLOGY,2016,214(AUG):653-659.
APA Olajuyin, Ayobami Matthew.,Yang, Maohua.,Liu, Yilan.,Mu, Tingzhen.,Tian, Jiangnan.,...&Xing, Jianmin.(2016).Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli.BIORESOURCE TECHNOLOGY,214(AUG),653-659.
MLA Olajuyin, Ayobami Matthew,et al."Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli".BIORESOURCE TECHNOLOGY 214.AUG(2016):653-659.
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