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Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli
Olajuyin, Ayobami Matthew1,2; Yang, Maohua1; Liu, Yilan1; Mu, Tingzhen1,2; Tian, Jiangnan1,2; Adaramoye, Oluwatosin Adekunle3; Xing, Jianmin1
2016-08-01
Source PublicationBIORESOURCE TECHNOLOGY
ISSN0960-8524
Volume214Issue:AUGPages:653-659
AbstractSuccinic acid, a C4 dicarboxylic acid is used in many fields such as food, agriculture, pharmaceutical and polymer industries. In this study, microbial production of succinic acid from Palmaria palmata was investigated for the first time. In engineered Escherichia coli KLPPP, lactate dehydrogenase, pyruvate formate lyase, phosphotransacetylase-acetate kinase and pyruvate oxidase genes were deleted while phosphoenolpyruvate carboxykinase was overexpressed. The recombinant exhibited higher molar yield of succinic acid on galactose (1.20 +/- 0.02 mol/mol) than glucose (0.48 +/- 0.03 mol/mol). The concentration and molar yield of succinic acid were 22.40 +/- 0.12 g/L and 1.13 +/- 0.02 mol/mol total sugar respectively after 72 h dual phase fermentation from P. palmata hydrolysate which composed of glucose (12.57 +/- 0.17 g/L) and galactose (18.03 +/- 0.10 g/L). The results demonstrate that P. palmata red macroalgae biomass represents a novel and an economically alternative feedstock for biochemicals production. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordPalmaria Palmata Escherichia Coli Succinic Acid Galactose Fermentation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.biortech.2016.04.117
Indexed BySCI
Language英语
WOS KeywordANAEROBIOSPIRILLUM-SUCCINICIPRODUCENS ; BIOFUEL PRODUCTION ; DIAUXIC GROWTH ; FERMENTATION ; MACROALGAE ; GALACTOSE ; GLUCOSE ; STRAIN ; PATHWAYS ; MUTATION
WOS Research AreaAgriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectAgricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
Funding OrganizationNational High Technology Research and Development Program of China (863 Project)(2014AA021905)
WOS IDWOS:000377366900083
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Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/20957
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Univ Ibadan, Dept Biochem, Fac Basic Med Sci, Ibadan, Nigeria
Recommended Citation
GB/T 7714
Olajuyin, Ayobami Matthew,Yang, Maohua,Liu, Yilan,et al. Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli[J]. BIORESOURCE TECHNOLOGY,2016,214(AUG):653-659.
APA Olajuyin, Ayobami Matthew.,Yang, Maohua.,Liu, Yilan.,Mu, Tingzhen.,Tian, Jiangnan.,...&Xing, Jianmin.(2016).Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli.BIORESOURCE TECHNOLOGY,214(AUG),653-659.
MLA Olajuyin, Ayobami Matthew,et al."Efficient production of succinic acid from Palmaria palmata hydrolysate by metabolically engineered Escherichia coli".BIORESOURCE TECHNOLOGY 214.AUG(2016):653-659.
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