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Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses
Liu, Qi1,2; Jia, Jilei1,2; Yang, Tingyuan1; Fan, Qingze1,2; Wang, Lianyan1; Ma, Guanghui1,3
2016-04-06
Source PublicationSMALL
ISSN1613-6810
Volume12Issue:13Pages:1744-1757
AbstractAiming to enhance the immunogenicity of subunit vaccines, a novel antigen delivery and adjuvant system based on dopamine polymerization on the surface of poly(d,l-lactic-glycolic-acid) nanoparticles (NPs) with multiple mechanisms of immunity enhancement is developed. The mussel-inspired biomimetic polydopamine (pD) not only serves as a coating to NPs but also functionalizes NP surfaces. The method is facile and mild including simple incubation of the preformed NPs in the weak alkaline dopamine solution, and incorporation of hepatitis B surface antigen and TLR9 agonist unmethylated cytosine-guanine (CpG) motif with the pD surface. The as-constructed NPs possess pathogen-mimicking manners owing to their size, shape, and surface molecular immune-activating properties given by CpG. The biocompatibility and biosafety of these pathogen-mimicking NPs are confirmed using bone marrow-derived dendritic cells. Pathogen-mimicking NPs hold great potential as vaccine delivery and adjuvant system due to their ability to: 1) enhance cytokine secretion and immune cell recruitment at the injection site; 2) significantly activate and maturate dendritic cells; 3) induce stronger humoral and cellular immune responses in vivo. Furthermore, this simple and versatile dopamine polymerization method can be applicable to endow NPs with characteristics to mimic pathogen structure and function, and manipulate NPs for the generation of efficacious vaccine adjuvants.
KeywordDopamine Polymerization Pathogen-mimicking Polymeric Nanoparticles Surface Modification Vaccine Delivery And Adjuvant Systems
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
DOI10.1002/smll.201503662
Indexed BySCI
Language英语
WOS KeywordDENDRITIC CELLS ; INNATE IMMUNITY ; IN-VIVO ; SURFACE MODIFICATION ; BACTERIAL-DNA ; HIV-1 P24 ; CPG DNA ; DELIVERY ; POLYDOPAMINE ; ANTIGEN
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
Funding OrganizationNational Science Foundation of China(21476243) ; 973 Program(2013CB531500) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KSCX2-EW-R-19)
WOS IDWOS:000373733200010
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/20982
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Natl Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chem Sci & Engn Tianjin, Collaborat Innovat Ctr, Tianjin 300072, Peoples R China
Recommended Citation
GB/T 7714
Liu, Qi,Jia, Jilei,Yang, Tingyuan,et al. Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses[J]. SMALL,2016,12(13):1744-1757.
APA Liu, Qi,Jia, Jilei,Yang, Tingyuan,Fan, Qingze,Wang, Lianyan,&Ma, Guanghui.(2016).Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses.SMALL,12(13),1744-1757.
MLA Liu, Qi,et al."Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses".SMALL 12.13(2016):1744-1757.
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