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Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses
Liu, Qi1,2; Jia, Jilei1,2; Yang, Tingyuan1; Fan, Qingze1,2; Wang, Lianyan1; Ma, Guanghui1,3
2016-04-06
发表期刊SMALL
ISSN1613-6810
卷号12期号:13页码:1744-1757
摘要Aiming to enhance the immunogenicity of subunit vaccines, a novel antigen delivery and adjuvant system based on dopamine polymerization on the surface of poly(d,l-lactic-glycolic-acid) nanoparticles (NPs) with multiple mechanisms of immunity enhancement is developed. The mussel-inspired biomimetic polydopamine (pD) not only serves as a coating to NPs but also functionalizes NP surfaces. The method is facile and mild including simple incubation of the preformed NPs in the weak alkaline dopamine solution, and incorporation of hepatitis B surface antigen and TLR9 agonist unmethylated cytosine-guanine (CpG) motif with the pD surface. The as-constructed NPs possess pathogen-mimicking manners owing to their size, shape, and surface molecular immune-activating properties given by CpG. The biocompatibility and biosafety of these pathogen-mimicking NPs are confirmed using bone marrow-derived dendritic cells. Pathogen-mimicking NPs hold great potential as vaccine delivery and adjuvant system due to their ability to: 1) enhance cytokine secretion and immune cell recruitment at the injection site; 2) significantly activate and maturate dendritic cells; 3) induce stronger humoral and cellular immune responses in vivo. Furthermore, this simple and versatile dopamine polymerization method can be applicable to endow NPs with characteristics to mimic pathogen structure and function, and manipulate NPs for the generation of efficacious vaccine adjuvants.
关键词Dopamine Polymerization Pathogen-mimicking Polymeric Nanoparticles Surface Modification Vaccine Delivery And Adjuvant Systems
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
DOI10.1002/smll.201503662
收录类别SCI
语种英语
关键词[WOS]DENDRITIC CELLS ; INNATE IMMUNITY ; IN-VIVO ; SURFACE MODIFICATION ; BACTERIAL-DNA ; HIV-1 P24 ; CPG DNA ; DELIVERY ; POLYDOPAMINE ; ANTIGEN
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
项目资助者National Science Foundation of China(21476243) ; 973 Program(2013CB531500) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KSCX2-EW-R-19)
WOS记录号WOS:000373733200010
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20982
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Natl Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Inst Proc Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chem Sci & Engn Tianjin, Collaborat Innovat Ctr, Tianjin 300072, Peoples R China
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Liu, Qi,Jia, Jilei,Yang, Tingyuan,et al. Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses[J]. SMALL,2016,12(13):1744-1757.
APA Liu, Qi,Jia, Jilei,Yang, Tingyuan,Fan, Qingze,Wang, Lianyan,&Ma, Guanghui.(2016).Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses.SMALL,12(13),1744-1757.
MLA Liu, Qi,et al."Pathogen-Mimicking Polymeric Nanoparticles based on Dopamine Polymerization as Vaccines Adjuvants Induce Robust Humoral and Cellular Immune Responses".SMALL 12.13(2016):1744-1757.
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