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Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide
Li, Qiang1; Zhao, Lan1; Zhang, Rongyue2; Huang, Yongdong1; Zhang, Yan1; Zhang, Kun1,3; Wu, Xuexing1; Zhang, Zhigang3; Gong, Fangling1; Su, Zhiguo1; Ma, Guanghui1
2016-04-01
发表期刊JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
卷号1017期号:APR页码:129-135
摘要A high cross-linking polystyrene(PSt)-based anion-exchange material with uniformly size, high ion exchange capacity, and high hydrophilicity was synthesized by a novel surface functionalization approach in this study. Uniformly sized PSt microspheres were prepared by the membrane emulsion polymerization strategy, and then modified by (1) conversing resid ual surface vinyl groups to epoxy groups followed by quaternization, and (2) decorating aromatic ring matrix including nitration, reduction and attachment of glycidyltrimethylammonium chloride. The 3-D morphology and porous features of microspheres were observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The surface of the modified PSt became roughness but the particle size remained same. Meanwhile, FT-IR spectra and laser scanning confocal microscope (LCSM) indicated that the modification groups had been successfully covalently coated onto the PSt microspheres. Modified PSt microspheres showed greatly improved hydrophilicity and biocompatibility with 0.387 mmol/mL ion exchange capacity (IEC). In the application evaluation procedure, exenatide can be purified from 42.9% (peptide crudes) to 88.6% by modified PSt column with 97.1% recovery yield. This modified PSt microspheres had a large potential in application for efficient separation of peptides. (C) 2016 Elsevier B.V. All rights reserved.
关键词Polystyrene Microspheres Hydrophilicity Anion-exchange Peptide Separation
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.jchromb.2016.02.044
收录类别SCI
语种英语
关键词[WOS]FRIEDEL-CRAFTS REACTION ; EXCHANGE CHROMATOGRAPHY ; GRAM SCALE ; ANALOG ; PURIFICATION ; PARTICLES ; EXENDIN-4 ; CATALYSTS ; PROTEINS ; CHLORIDE
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
项目资助者National Natural Science Foundation of China(21206175 ; National Key Basic Research Program of China(2013BAB01B03 ; 51103158 ; 2014AA021005 ; 21476241 ; 2014AA021006) ; 21306207 ; 21306206 ; 51403213)
WOS记录号WOS:000373547200016
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20985
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Beijing Inst Petrochem Technol, Dept Chem Engn, Beijing 102617, Peoples R China
3.Hebei Normal Univ Sci & Technol, Qinhuangdao 066004, Hebei, Peoples R China
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Li, Qiang,Zhao, Lan,Zhang, Rongyue,et al. Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1017(APR):129-135.
APA Li, Qiang.,Zhao, Lan.,Zhang, Rongyue.,Huang, Yongdong.,Zhang, Yan.,...&Ma, Guanghui.(2016).Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1017(APR),129-135.
MLA Li, Qiang,et al."Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1017.APR(2016):129-135.
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