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Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide
Li, Qiang1; Zhao, Lan1; Zhang, Rongyue2; Huang, Yongdong1; Zhang, Yan1; Zhang, Kun1,3; Wu, Xuexing1; Zhang, Zhigang3; Gong, Fangling1; Su, Zhiguo1; Ma, Guanghui1
2016-04-01
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume1017Issue:APRPages:129-135
AbstractA high cross-linking polystyrene(PSt)-based anion-exchange material with uniformly size, high ion exchange capacity, and high hydrophilicity was synthesized by a novel surface functionalization approach in this study. Uniformly sized PSt microspheres were prepared by the membrane emulsion polymerization strategy, and then modified by (1) conversing resid ual surface vinyl groups to epoxy groups followed by quaternization, and (2) decorating aromatic ring matrix including nitration, reduction and attachment of glycidyltrimethylammonium chloride. The 3-D morphology and porous features of microspheres were observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The surface of the modified PSt became roughness but the particle size remained same. Meanwhile, FT-IR spectra and laser scanning confocal microscope (LCSM) indicated that the modification groups had been successfully covalently coated onto the PSt microspheres. Modified PSt microspheres showed greatly improved hydrophilicity and biocompatibility with 0.387 mmol/mL ion exchange capacity (IEC). In the application evaluation procedure, exenatide can be purified from 42.9% (peptide crudes) to 88.6% by modified PSt column with 97.1% recovery yield. This modified PSt microspheres had a large potential in application for efficient separation of peptides. (C) 2016 Elsevier B.V. All rights reserved.
KeywordPolystyrene Microspheres Hydrophilicity Anion-exchange Peptide Separation
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.jchromb.2016.02.044
Indexed BySCI
Language英语
WOS KeywordFRIEDEL-CRAFTS REACTION ; EXCHANGE CHROMATOGRAPHY ; GRAM SCALE ; ANALOG ; PURIFICATION ; PARTICLES ; EXENDIN-4 ; CATALYSTS ; PROTEINS ; CHLORIDE
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
Funding OrganizationNational Natural Science Foundation of China(21206175 ; National Key Basic Research Program of China(2013BAB01B03 ; 51103158 ; 2014AA021005 ; 21476241 ; 2014AA021006) ; 21306207 ; 21306206 ; 51403213)
WOS IDWOS:000373547200016
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/20985
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Beijing Inst Petrochem Technol, Dept Chem Engn, Beijing 102617, Peoples R China
3.Hebei Normal Univ Sci & Technol, Qinhuangdao 066004, Hebei, Peoples R China
Recommended Citation
GB/T 7714
Li, Qiang,Zhao, Lan,Zhang, Rongyue,et al. Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1017(APR):129-135.
APA Li, Qiang.,Zhao, Lan.,Zhang, Rongyue.,Huang, Yongdong.,Zhang, Yan.,...&Ma, Guanghui.(2016).Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1017(APR),129-135.
MLA Li, Qiang,et al."Functional hydrophilic polystyrene beads with uniformly size and high cross-linking degree facilitated rapid separation of exenatide".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1017.APR(2016):129-135.
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