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Thermo- and pH-responsive polymer brushes-grafted gigaporous polystyrene microspheres as a high-speed protein chromatography matrix
Qu, Jian-Bo1; Xu, Yu-Liang1; Liu, Jun-Yi1; Zeng, Jing-Bin1; Chen, Yan-Li1; Zhou, Wei-Qing2; Liu, Jian-Guo1
2016-04-08
发表期刊JOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
卷号1441期号:APR页码:60-67
摘要Dual thermo- and pH-responsive chromatography has been proposed using poly(N-isopropylacrylamid-eco-butyl methacrylate-co-N,N-dimethylaminopropyl acrylamide) (P(NIPAM-co-BMA-co-DMAPAAM)) brushes grafted gigaporous polystyrene microspheres (GPM) as matrix. Atom transfer radical polymerization (ATRP) initiator was first coupled onto GPM through Friedel-Crafts acylation with 2-bromoisobutyryl bromide. The dual-responsive polymer brushes were then grafted onto GPM via surface-initiated ATRP. The surface composition, gigaporous structure, protein adsorption and dual-responsive chromatographic properties of the matrix (GPM-P(NIPAM-co-BMA-co-DMAPAAM) were characterized in detail. Results showed that GPM were successfully grafted with thermoresponsive cationic polymer brushes and that the gigaporous structure was well maintained. A column packed with GPM-P(NIPAM-co-BMA-co-DMAPAAM presented low backpressure, good permeability and appreciable thermo-responsibility. By changing pH of the mobile phase and temperature of the column in turn, the column can separate three model proteins at the mobile phase velocity up to 2528 cm h(-1). A separation mechanism of this matrix was also proposed. All results indicate that the dual thermo- and pH-responsive chromatography matrix has great potentials in 'green' high-speed protein chromatography. (C) 2016 Elsevier B.V. All rights reserved.
关键词Gigaporous Polystyrene Microspheres Thermo- And Ph-responsive Polymer Brushes Protein Separation High-speed Protein Chromatography Atom Transfer Radical Polymerization
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2016.02.072
收录类别SCI
语种英语
关键词[WOS]STATIONARY-PHASE ; PERFUSION CHROMATOGRAPHY ; AQUEOUS CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; SEPARATION ; PEPTIDES ; BIOMOLECULES ; ADSORPTION ; SUPPORTS ; LIGAND
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
项目资助者National Natural Science Foundation of China(21176257 ; key Science and Technology Program of Qingdao Economic & Technical Development Zone(2013-1-56) ; 21473256 ; 21276280)
WOS记录号WOS:000373552300008
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/20990
专题生化工程国家重点实验室
作者单位1.China Univ Petr East China, Ctr Bioengn & Biotechnol, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
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Qu, Jian-Bo,Xu, Yu-Liang,Liu, Jun-Yi,et al. Thermo- and pH-responsive polymer brushes-grafted gigaporous polystyrene microspheres as a high-speed protein chromatography matrix[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1441(APR):60-67.
APA Qu, Jian-Bo.,Xu, Yu-Liang.,Liu, Jun-Yi.,Zeng, Jing-Bin.,Chen, Yan-Li.,...&Liu, Jian-Guo.(2016).Thermo- and pH-responsive polymer brushes-grafted gigaporous polystyrene microspheres as a high-speed protein chromatography matrix.JOURNAL OF CHROMATOGRAPHY A,1441(APR),60-67.
MLA Qu, Jian-Bo,et al."Thermo- and pH-responsive polymer brushes-grafted gigaporous polystyrene microspheres as a high-speed protein chromatography matrix".JOURNAL OF CHROMATOGRAPHY A 1441.APR(2016):60-67.
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