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Efficient fabrication of high-capacity immobilized metal ion affinity chromatographic media: The role of the dextran-grafting process and its manipulation
Zhao, Lan1; Zhang, Jingfei1,2; Huang, Yongdong1; Li, Qiang1; Zhang, Rongyue3; Zhu, Kai1,4; Suo, Jia3; Su, Zhiguo1; Zhang, Zhigang2; Ma, Guanghui1
2016-03-01
Source PublicationJOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
Volume39Issue:6Pages:1130-1136
AbstractNovel high-capacity Ni2+ immobilized metal ion affinity chromatographic media were prepared through the dextran-grafting process. Dextran was grafted to an allyl-activated agarose-based matrix followed by functionalization for the immobilized metal ion affinity chromatographic media. With elaborate regulation of the allylation degree, dextran was completely or partly grafted to agarose microspheres, namely, completely dextran-grafted agarose microspheres and partly dextran-grafted ones, respectively. Confocal laser scanning microscope results demonstrated that a good adjustment of dextran-grafting degree was achieved, and dextran was distributed uniformly in whole completely dextran-grafted microspheres, while just distributed around the outside of the partly dextran-grafted ones. Flow hydrodynamic properties were improved greatly after the dextran-grafting process, and the flow velocity increased by about 30% compared with that of a commercial chromatographic medium (Ni Sepharose FF). A significant improvement of protein binding performance was also achieved by the dextran-grafting process, and partly dextran-grafted Ni2+ chelating medium had a maximum binding capacity for His-tagged lactate dehydrogenase about 2.5 times higher than that of Ni Sepharose FF. The results indicated that this novel chromatographic medium is promising for applications in high-efficiency and large-scale protein purification.
KeywordDextran Immobilized Metal Ion Affinity Chromatography Nickel Protein Purification
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1002/jssc.201501291
Indexed BySCI
Language英语
WOS KeywordEXCHANGE CHROMATOGRAPHY ; IMMUNOGLOBULIN-G ; AGAROSE MEDIA ; PURIFICATION ; ADSORPTION ; PROTEINS ; ADSORBENT ; RESINS ; TAGS ; GELS
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
Funding OrganizationNatural Sciences Foundation of China(21306206 ; National Key Technology R&D Program of the Ministry of Science and Technology(2013BAB01B03) ; National High Technology Research and Development Program of China (863 Program)(2014AA021006) ; National Key Scientific Instrument and Equipment Development Project(2013YQ14040502) ; 21206175 ; 21476241)
WOS IDWOS:000373617100013
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/20991
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Hebei Normal Univ Sci & Technol, Qinhuangdao, Hebei, Peoples R China
3.Beijing Inst Petrochem Technol, Dept Chem Engn, Beijing, Peoples R China
4.China Univ Min & Technol, Sch Chem & Environm Engn, Beijing, Peoples R China
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GB/T 7714
Zhao, Lan,Zhang, Jingfei,Huang, Yongdong,et al. Efficient fabrication of high-capacity immobilized metal ion affinity chromatographic media: The role of the dextran-grafting process and its manipulation[J]. JOURNAL OF SEPARATION SCIENCE,2016,39(6):1130-1136.
APA Zhao, Lan.,Zhang, Jingfei.,Huang, Yongdong.,Li, Qiang.,Zhang, Rongyue.,...&Ma, Guanghui.(2016).Efficient fabrication of high-capacity immobilized metal ion affinity chromatographic media: The role of the dextran-grafting process and its manipulation.JOURNAL OF SEPARATION SCIENCE,39(6),1130-1136.
MLA Zhao, Lan,et al."Efficient fabrication of high-capacity immobilized metal ion affinity chromatographic media: The role of the dextran-grafting process and its manipulation".JOURNAL OF SEPARATION SCIENCE 39.6(2016):1130-1136.
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