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Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres
Wang, Weichen1,2; Zhou, Weiqing1; Wei, Wei1; Li, Juan1; Hao, Dongxia1; Su, Zhiguo1; Ma, Guanghui1
2016-04-01
发表期刊POLYMERS
ISSN2073-4360
卷号8期号:4页码:116
摘要Compared with the one immobilized in the conventional mesoporous microspheres, the enzyme immobilized in gigaporous microspheres showed much higher activity and better stability. To gain a deeper understanding, we herein selected lipase as a prototype to comparatively analyze the adsorption behavior of lipase at interfaces in gigaporous and mesoporous polystyrene microspheres at very low lipase concentration, and further compared with the adsorption on a completely flat surface (a chip). Owing to the limited space of narrow pores, lipase molecules were inclined to be adsorbed as a monolayer in mesoporous microspheres. During this process, the interaction between lipase molecules and the interface was stronger, which could result in the structural change of lipase molecular and compromised specific activity. In addition to monolayer adsorption, more multilayer adsorption of enzyme molecules also occurred in gigaporous microspheres. Besides the adsorption state, the pore curvature also affected the lipase adsorption. Due to the multilayer adsorption, the excellent mass transfer properties for the substrate and the product in the large pores, and the small pore curvature, lipase immobilized in gigaporous microspheres showed better behaviors.
关键词Lipase Adsorption Gigaporous Mesoporous Microsphere Qcm-d
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
DOI10.3390/polym8040116
收录类别SCI
语种英语
关键词[WOS]QUARTZ-CRYSTAL MICROBALANCE ; CANDIDA-RUGOSA LIPASE ; PORE-SIZE ; IMMOBILIZED ENZYMES ; MESOPOROUS SILICA ; POLYESTER SYNTHESIS ; CATALYTIC-ACTIVITY ; PARTICLE-SIZE ; B ADSORPTION ; ANTARCTICA
WOS研究方向Polymer Science
WOS类目Polymer Science
项目资助者National Natural Science Foundation of China(21336010 ; National Science and Technology Support Program(2012BAD32B09) ; 863 Project(2012AA02A406) ; 21106161)
WOS记录号WOS:000375159900021
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21017
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wang, Weichen,Zhou, Weiqing,Wei, Wei,et al. Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres[J]. POLYMERS,2016,8(4):116.
APA Wang, Weichen.,Zhou, Weiqing.,Wei, Wei.,Li, Juan.,Hao, Dongxia.,...&Ma, Guanghui.(2016).Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres.POLYMERS,8(4),116.
MLA Wang, Weichen,et al."Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres".POLYMERS 8.4(2016):116.
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