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Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres
Wang, Weichen1,2; Zhou, Weiqing1; Wei, Wei1; Li, Juan1; Hao, Dongxia1; Su, Zhiguo1; Ma, Guanghui1
2016-04-01
Source PublicationPOLYMERS
ISSN2073-4360
Volume8Issue:4Pages:116
AbstractCompared with the one immobilized in the conventional mesoporous microspheres, the enzyme immobilized in gigaporous microspheres showed much higher activity and better stability. To gain a deeper understanding, we herein selected lipase as a prototype to comparatively analyze the adsorption behavior of lipase at interfaces in gigaporous and mesoporous polystyrene microspheres at very low lipase concentration, and further compared with the adsorption on a completely flat surface (a chip). Owing to the limited space of narrow pores, lipase molecules were inclined to be adsorbed as a monolayer in mesoporous microspheres. During this process, the interaction between lipase molecules and the interface was stronger, which could result in the structural change of lipase molecular and compromised specific activity. In addition to monolayer adsorption, more multilayer adsorption of enzyme molecules also occurred in gigaporous microspheres. Besides the adsorption state, the pore curvature also affected the lipase adsorption. Due to the multilayer adsorption, the excellent mass transfer properties for the substrate and the product in the large pores, and the small pore curvature, lipase immobilized in gigaporous microspheres showed better behaviors.
KeywordLipase Adsorption Gigaporous Mesoporous Microsphere Qcm-d
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.3390/polym8040116
Indexed BySCI
Language英语
WOS KeywordQUARTZ-CRYSTAL MICROBALANCE ; CANDIDA-RUGOSA LIPASE ; PORE-SIZE ; IMMOBILIZED ENZYMES ; MESOPOROUS SILICA ; POLYESTER SYNTHESIS ; CATALYTIC-ACTIVITY ; PARTICLE-SIZE ; B ADSORPTION ; ANTARCTICA
WOS Research AreaPolymer Science
WOS SubjectPolymer Science
Funding OrganizationNational Natural Science Foundation of China(21336010 ; National Science and Technology Support Program(2012BAD32B09) ; 863 Project(2012AA02A406) ; 21106161)
WOS IDWOS:000375159900021
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21017
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Weichen,Zhou, Weiqing,Wei, Wei,et al. Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres[J]. POLYMERS,2016,8(4):116.
APA Wang, Weichen.,Zhou, Weiqing.,Wei, Wei.,Li, Juan.,Hao, Dongxia.,...&Ma, Guanghui.(2016).Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres.POLYMERS,8(4),116.
MLA Wang, Weichen,et al."Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres".POLYMERS 8.4(2016):116.
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