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Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6
Wang, Xiaohui1,2,3; Chi, Naiyu3; Bai, Fengwu1; Du, Yuguang4; Zhao, Yong2; Yin, Heng2
2016-03-01
发表期刊EXTREMOPHILES
ISSN1431-0651
卷号20期号:2页码:167-176
摘要We have previously reported a non-processive endo-type chitinase, ChiA, from a newly isolated marine psychrophilic bacterium, Pseudoalteromonas sp. DL-6. In this study, a processive exo-type chitinase, ChiC, was cloned from the same bacterium and characterized in detail. ChiC could hydrolyze crystalline chitin into (GlcNAc)(2) as the only observed product. It exhibited high catalytic activity even at low temperatures, e.g. close to 0 degrees C, or in the presence of 5 M NaCl, suggesting that ChiC was a cold-adapted and highly salt-tolerant chitinase. ChiC could also hydrolyze other substrates, including chitosan and Avicel, indicating its broad substrate specificity. Sequence features indicated that ChiC was a multi-domain protein having a deep substrate-binding groove that was regarded as characteristic of processive exo-chitinases. Enzymatic hydrolysis of chitin by ChiC could be remarkably boosted in the presence of ChiA, suggesting the synergy of ChiC and ChiA. This work provided a new evidence to prove that marine psychrophilic bacteria utilized a synergistic enzyme system to degrade recalcitrant chitin.
关键词Chitin Exochitinase Cold-adapted Salt-tolerant Pseudoalteromonas Sp.
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00792-016-0810-5
收录类别SCI
语种英语
关键词[WOS]MARINE PSYCHROPHILIC BACTERIUM ; SP STRAIN O-7 ; SERRATIA-MARCESCENS ; RECALCITRANT POLYSACCHARIDES ; BIOCHEMICAL-CHARACTERIZATION ; MORITELLA-MARINA ; BINDING DOMAIN ; BETA-CHITIN ; DEGRADATION ; PROCESSIVITY
WOS研究方向Biochemistry & Molecular Biology ; Microbiology
WOS类目Biochemistry & Molecular Biology ; Microbiology
项目资助者program for National High-tech Research and Development Program(2014AA093604) ; National Science Foundation(31500039 ; National High-tech Research and Development Program(2012AA021501) ; Key Deployment Program of Chinese Academy of Sciences(KSZD-EW-Z-015-2) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2015144) ; 31300668)
WOS记录号WOS:000374832600006
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21037
专题管理及支撑部门
作者单位1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycobiotechnol Res Grp, Dalian 116023, Peoples R China
3.Dalian Univ, Sch Life Sci & Biotechnol, Dalian 116622, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
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Wang, Xiaohui,Chi, Naiyu,Bai, Fengwu,et al. Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6[J]. EXTREMOPHILES,2016,20(2):167-176.
APA Wang, Xiaohui,Chi, Naiyu,Bai, Fengwu,Du, Yuguang,Zhao, Yong,&Yin, Heng.(2016).Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6.EXTREMOPHILES,20(2),167-176.
MLA Wang, Xiaohui,et al."Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6".EXTREMOPHILES 20.2(2016):167-176.
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