| Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6 | |
| Wang, Xiaohui1,2,3; Chi, Naiyu3; Bai, Fengwu1; Du, Yuguang4; Zhao, Yong2; Yin, Heng2 | |
| 2016-03-01 | |
| 发表期刊 | EXTREMOPHILES
 |
| ISSN | 1431-0651 |
| 卷号 | 20期号:2页码:167-176 |
| 摘要 | We have previously reported a non-processive endo-type chitinase, ChiA, from a newly isolated marine psychrophilic bacterium, Pseudoalteromonas sp. DL-6. In this study, a processive exo-type chitinase, ChiC, was cloned from the same bacterium and characterized in detail. ChiC could hydrolyze crystalline chitin into (GlcNAc)(2) as the only observed product. It exhibited high catalytic activity even at low temperatures, e.g. close to 0 degrees C, or in the presence of 5 M NaCl, suggesting that ChiC was a cold-adapted and highly salt-tolerant chitinase. ChiC could also hydrolyze other substrates, including chitosan and Avicel, indicating its broad substrate specificity. Sequence features indicated that ChiC was a multi-domain protein having a deep substrate-binding groove that was regarded as characteristic of processive exo-chitinases. Enzymatic hydrolysis of chitin by ChiC could be remarkably boosted in the presence of ChiA, suggesting the synergy of ChiC and ChiA. This work provided a new evidence to prove that marine psychrophilic bacteria utilized a synergistic enzyme system to degrade recalcitrant chitin. |
| 关键词 | Chitin Exochitinase Cold-adapted Salt-tolerant Pseudoalteromonas Sp. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s00792-016-0810-5 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MARINE PSYCHROPHILIC BACTERIUM ; SP STRAIN O-7 ; SERRATIA-MARCESCENS ; RECALCITRANT POLYSACCHARIDES ; BIOCHEMICAL-CHARACTERIZATION ; MORITELLA-MARINA ; BINDING DOMAIN ; BETA-CHITIN ; DEGRADATION ; PROCESSIVITY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Microbiology |
| WOS类目 | Biochemistry & Molecular Biology ; Microbiology |
| 项目资助者 | program for National High-tech Research and Development Program(2014AA093604) ; National Science Foundation(31500039 ; National High-tech Research and Development Program(2012AA021501) ; Key Deployment Program of Chinese Academy of Sciences(KSZD-EW-Z-015-2) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2015144) ; 31300668) |
| WOS记录号 | WOS:000374832600006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ipe.ac.cn/handle/122111/21037 |
| 专题 | 管理及支撑部门 |
| 作者单位 | 1.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Nat Prod & Glycobiotechnol Res Grp, Dalian 116023, Peoples R China 3.Dalian Univ, Sch Life Sci & Biotechnol, Dalian 116622, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaohui,Chi, Naiyu,Bai, Fengwu,et al. Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6[J]. EXTREMOPHILES,2016,20(2):167-176. |
| APA | Wang, Xiaohui,Chi, Naiyu,Bai, Fengwu,Du, Yuguang,Zhao, Yong,&Yin, Heng.(2016).Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6.EXTREMOPHILES,20(2),167-176. |
| MLA | Wang, Xiaohui,et al."Characterization of a cold-adapted and salt-tolerant exo-chitinase (ChiC) from Pseudoalteromonas sp DL-6".EXTREMOPHILES 20.2(2016):167-176. |
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