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Effects of mixed carbon sources on galactose and mannose content of exopolysaccharides and related enzyme activities in Ganoderma lucidum
Peng, Lin1,4; Li, Jie1,4; Liu, Ying3; Xu, Zhenghong1; Wu, Jian-Yong2; Ding, Zhongyang1,4; Gu, Zhenghua1,4; Zhang, Liang1,4; Shi, Guiyang1,4
2016
Source PublicationRSC ADVANCES
ISSN2046-2069
Volume6Issue:45Pages:39284-39291
AbstractThis work investigated the effects of mixed carbon sources on the monosaccharide composition of exopolysaccharides (EPS) and activity of related enzymes, phosphoglucose isomerase (PGI), alpha-phosphoglucomutase (PGM), phosphomannose isomerase (PMI) and GDP-D-Man pyrophosphorylase (GMP), in the EPS biosynthesis by Ganoderma lucidum. Combinations of two carbon sources, glucose (Glc) + mannose (Man) and Glc + galactose (Gal), were applied at various mass ratios in the liquid culture of G. lucidum. The results showed that Glc, Gal, and Man were the major monosaccharides in G. lucidum EPS, and the combined mole percentages of monosaccharides were >80%, the same as those in the carbon source mixture. Activities of PGI, PMI, and GMP were correlated with Man mole percentage in EPSs and were enhanced by increasing the proportion of Gal for the carbon source Glc + Gal. PGM activity was correlated with Gal mole percentage and was reduced by increasing the Man proportion for Glc + Man. The expression of four genes encoding PGI, PMI, GMP and PGM reached peak levels on day 6 of culture, whereas the enzyme activities increased steadily from day 3 to 8. The monosaccharide composition of G. lucidum EPS for various mixed carbon source conditions thus appeared to be controlled by the translational level of genes encoding PGM, PGI, PMI, and GMP. These findings will be helpful to control the monosaccharide composition for desired biological activity of EPS produced by G. lucidum fermentation.
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1039/c6ra04798j
Indexed BySCI
Language英语
WOS KeywordMONOSACCHARIDE COMPOSITION ; POLYSACCHARIDE ; PATHWAYS ; BIOSYNTHESIS ; EXTRACTION ; CULTURE ; PROTEIN ; STRAIN
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
Funding OrganizationNational Natural Science Foundation of China(31271918 ; National High Technology Research and Development Program of China 863 Program(2012AA021505) ; Fundamental Research Funds for the Central Universities(JUSRP51411B) ; Key Laboratory of Carbohydrate Chemistry and Biotechnology(KLCCB-KF201205) ; 21206177)
WOS IDWOS:000374972800096
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21043
Collection生化工程国家重点实验室
Affiliation1.Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Carbohydrate Chem & Biotechnol, Wuxi 214122, Peoples R China
2.Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
4.Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China
Recommended Citation
GB/T 7714
Peng, Lin,Li, Jie,Liu, Ying,et al. Effects of mixed carbon sources on galactose and mannose content of exopolysaccharides and related enzyme activities in Ganoderma lucidum[J]. RSC ADVANCES,2016,6(45):39284-39291.
APA Peng, Lin.,Li, Jie.,Liu, Ying.,Xu, Zhenghong.,Wu, Jian-Yong.,...&Shi, Guiyang.(2016).Effects of mixed carbon sources on galactose and mannose content of exopolysaccharides and related enzyme activities in Ganoderma lucidum.RSC ADVANCES,6(45),39284-39291.
MLA Peng, Lin,et al."Effects of mixed carbon sources on galactose and mannose content of exopolysaccharides and related enzyme activities in Ganoderma lucidum".RSC ADVANCES 6.45(2016):39284-39291.
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