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Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine
Xia, Ting-Ting; Liu, Chun-Zhao1; Hu, Jian-Hua; Guo, Chen1
2016-07-01
Source PublicationCHEMICAL ENGINEERING JOURNAL
ISSN1385-8947
Volume295Issue:JULYPages:201-206
AbstractPolyethylenimine (PEI) as a spacer-arm polymer was modified on amine-functioned Fe3O4 nanoparticles (Fe3O4-NH2 NPs). PEI modified Fe3O4 nanoparticles (Fe304-NH2-PEI NPs) were chelated with Cu2+ to immobilize laccase through metal affinity adsorption. Meanwhile, PEI un-modified Fe3O4-NH2 NPs acted as a control group. The adsorption capacity of Fe304-NH2-PEI-Cu2+ NPs was larger than Fe3O4-NH2-Cu2+ NPs almost in the whole range of laccase concentration. And the activity recovery of Fe3O4-NH2-PEILaccase was also higher than Fe304-NH2-Laccase when the laccase concentration was lower than 26 pg/mL. The maximum activity recovery of Fe3O4-NH2-PEI-Laccase (107.41%) was much higher than Fe3O4-NH2-Laccase (42.75%). The corresponding specific activity of Fe3O4-NH2-PEI-Laccase and Fe3O4-NH2-Laccase was separately 101.33 and 74.45 times as large as free laccase product. The enzymatic properties of the two immobilized laccases were both improved and the immobilization realized reuse of laccase. The results showed that Fe3O4-NH2-PEI-Cu2+ NPs were more promising than Fe3O4NH2-Cu2+ NPs for purification and immobilization of laccase simultaneously and are potential for large-scale laccase immobilization. (C) 2016 Elsevier B.V. All rights reserved.
KeywordAmine-functioned Fe3o4 Nanoparticle Polyethylenimine Spacer-arm Cu2++ Chelated Laccase Immobilization
SubtypeArticle
WOS HeadingsScience & Technology ; Technology
DOI10.1016/j.cej.2016.03.044
Indexed BySCI
Language英语
WOS KeywordCHELATE AFFINITY INTERACTION ; TRAMETES-VERSICOLOR ; PHENOLIC-COMPOUNDS ; REVERSIBLE IMMOBILIZATION ; COMPOSITE ; DEGRADATION ; PARTICLES ; REMOVAL ; DYE ; CHITOSAN
WOS Research AreaEngineering
WOS SubjectEngineering, Environmental ; Engineering, Chemical
Funding OrganizationNational Basic Research Program (973 Program) of China(2013CB733600) ; National Natural Science Foundation of China(21476242) ; National Key Technology Research and Development Program of China(2015BAK45B01) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KSZD-EW-Z-015)
WOS IDWOS:000375507300022
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21072
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Xia, Ting-Ting,Liu, Chun-Zhao,Hu, Jian-Hua,et al. Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine[J]. CHEMICAL ENGINEERING JOURNAL,2016,295(JULY):201-206.
APA Xia, Ting-Ting,Liu, Chun-Zhao,Hu, Jian-Hua,&Guo, Chen.(2016).Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine.CHEMICAL ENGINEERING JOURNAL,295(JULY),201-206.
MLA Xia, Ting-Ting,et al."Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine".CHEMICAL ENGINEERING JOURNAL 295.JULY(2016):201-206.
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