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Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine
Xia, Ting-Ting; Liu, Chun-Zhao1; Hu, Jian-Hua; Guo, Chen1
2016-07-01
发表期刊CHEMICAL ENGINEERING JOURNAL
ISSN1385-8947
卷号295期号:JULY页码:201-206
摘要Polyethylenimine (PEI) as a spacer-arm polymer was modified on amine-functioned Fe3O4 nanoparticles (Fe3O4-NH2 NPs). PEI modified Fe3O4 nanoparticles (Fe304-NH2-PEI NPs) were chelated with Cu2+ to immobilize laccase through metal affinity adsorption. Meanwhile, PEI un-modified Fe3O4-NH2 NPs acted as a control group. The adsorption capacity of Fe304-NH2-PEI-Cu2+ NPs was larger than Fe3O4-NH2-Cu2+ NPs almost in the whole range of laccase concentration. And the activity recovery of Fe3O4-NH2-PEILaccase was also higher than Fe304-NH2-Laccase when the laccase concentration was lower than 26 pg/mL. The maximum activity recovery of Fe3O4-NH2-PEI-Laccase (107.41%) was much higher than Fe3O4-NH2-Laccase (42.75%). The corresponding specific activity of Fe3O4-NH2-PEI-Laccase and Fe3O4-NH2-Laccase was separately 101.33 and 74.45 times as large as free laccase product. The enzymatic properties of the two immobilized laccases were both improved and the immobilization realized reuse of laccase. The results showed that Fe3O4-NH2-PEI-Cu2+ NPs were more promising than Fe3O4NH2-Cu2+ NPs for purification and immobilization of laccase simultaneously and are potential for large-scale laccase immobilization. (C) 2016 Elsevier B.V. All rights reserved.
关键词Amine-functioned Fe3o4 Nanoparticle Polyethylenimine Spacer-arm Cu2++ Chelated Laccase Immobilization
文章类型Article
WOS标题词Science & Technology ; Technology
DOI10.1016/j.cej.2016.03.044
收录类别SCI
语种英语
关键词[WOS]CHELATE AFFINITY INTERACTION ; TRAMETES-VERSICOLOR ; PHENOLIC-COMPOUNDS ; REVERSIBLE IMMOBILIZATION ; COMPOSITE ; DEGRADATION ; PARTICLES ; REMOVAL ; DYE ; CHITOSAN
WOS研究方向Engineering
WOS类目Engineering, Environmental ; Engineering, Chemical
项目资助者National Basic Research Program (973 Program) of China(2013CB733600) ; National Natural Science Foundation of China(21476242) ; National Key Technology Research and Development Program of China(2015BAK45B01) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KSZD-EW-Z-015)
WOS记录号WOS:000375507300022
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21072
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China
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Xia, Ting-Ting,Liu, Chun-Zhao,Hu, Jian-Hua,et al. Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine[J]. CHEMICAL ENGINEERING JOURNAL,2016,295(JULY):201-206.
APA Xia, Ting-Ting,Liu, Chun-Zhao,Hu, Jian-Hua,&Guo, Chen.(2016).Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine.CHEMICAL ENGINEERING JOURNAL,295(JULY),201-206.
MLA Xia, Ting-Ting,et al."Improved performance of immobilized laccase on amine-functioned magnetic Fe3O4 nanoparticles modified with polyethylenimine".CHEMICAL ENGINEERING JOURNAL 295.JULY(2016):201-206.
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