CAS OpenIR  > 生化工程国家重点实验室
Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers
Fan, Jinxin1,2; Luo, Jianquan1; Chen, Xiangrong1; Wan, Yinhua1
2016-05-27
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1448Issue:MAYPages:121-126
AbstractPolydopamine, as an intermediate layer coated on PES membrane, was applied to fabricate various membrane adsorbers. Anion-exchange, hydrophobic interaction and affinity membrane adsorbers prepared by this facile method exhibited a high selectivity in fractionation of IgG (immunoglobulin)/HSA (human serum albumin) mixture. The anion-exchange membrane adsorber containing polyethylenimine (PEI) improved the HSA purity from 17.7% to 96.7%; The hydrophobic interaction membrane adsorber with Dodecyl mercaptan (DDM) as ligand obtained an IgG purity of 94.6%; Histidine attached affinity membrane chromatography achieved nearly a 100% purity of IgG. The present work indicated that the polydopamine layer not only activated membrane surface to attach various adsorptive ligands under the mild condition, but also reduced non-specific adsorption. Due to the versatile conjunction function, this facile mussel-inspired coating is also promising for the preparation of diverse membrane adsorbers. (C) 2016 Elsevier B.V. All rights reserved.
KeywordMembrane Adsorber Membrane Chromatography Ion-exchange Hydrophobic Interaction Affinity Membrane Adsorber
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2016.04.063
Indexed BySCI
Language英语
WOS KeywordPROTEIN SEPARATION ; SURFACE ; PURIFICATION ; POLYMER ; CHROMATOGRAPHY ; PERFORMANCE ; CAPACITY
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
Funding OrganizationNational High Technology Research and Development Program of China (863 Program)(2014AA021006) ; "100 Talents Program" of Chinese Academy of Sciences
WOS IDWOS:000376211600015
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21073
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Fan, Jinxin,Luo, Jianquan,Chen, Xiangrong,et al. Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1448(MAY):121-126.
APA Fan, Jinxin,Luo, Jianquan,Chen, Xiangrong,&Wan, Yinhua.(2016).Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers.JOURNAL OF CHROMATOGRAPHY A,1448(MAY),121-126.
MLA Fan, Jinxin,et al."Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers".JOURNAL OF CHROMATOGRAPHY A 1448.MAY(2016):121-126.
Files in This Item:
File Name/Size DocType Version Access License
Polydopamine meets p(1313KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Fan, Jinxin]'s Articles
[Luo, Jianquan]'s Articles
[Chen, Xiangrong]'s Articles
Baidu academic
Similar articles in Baidu academic
[Fan, Jinxin]'s Articles
[Luo, Jianquan]'s Articles
[Chen, Xiangrong]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Fan, Jinxin]'s Articles
[Luo, Jianquan]'s Articles
[Chen, Xiangrong]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.