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Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers
Fan, Jinxin1,2; Luo, Jianquan1; Chen, Xiangrong1; Wan, Yinhua1
2016-05-27
发表期刊JOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
卷号1448期号:MAY页码:121-126
摘要Polydopamine, as an intermediate layer coated on PES membrane, was applied to fabricate various membrane adsorbers. Anion-exchange, hydrophobic interaction and affinity membrane adsorbers prepared by this facile method exhibited a high selectivity in fractionation of IgG (immunoglobulin)/HSA (human serum albumin) mixture. The anion-exchange membrane adsorber containing polyethylenimine (PEI) improved the HSA purity from 17.7% to 96.7%; The hydrophobic interaction membrane adsorber with Dodecyl mercaptan (DDM) as ligand obtained an IgG purity of 94.6%; Histidine attached affinity membrane chromatography achieved nearly a 100% purity of IgG. The present work indicated that the polydopamine layer not only activated membrane surface to attach various adsorptive ligands under the mild condition, but also reduced non-specific adsorption. Due to the versatile conjunction function, this facile mussel-inspired coating is also promising for the preparation of diverse membrane adsorbers. (C) 2016 Elsevier B.V. All rights reserved.
关键词Membrane Adsorber Membrane Chromatography Ion-exchange Hydrophobic Interaction Affinity Membrane Adsorber
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2016.04.063
收录类别SCI
语种英语
关键词[WOS]PROTEIN SEPARATION ; SURFACE ; PURIFICATION ; POLYMER ; CHROMATOGRAPHY ; PERFORMANCE ; CAPACITY
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
项目资助者National High Technology Research and Development Program of China (863 Program)(2014AA021006) ; "100 Talents Program" of Chinese Academy of Sciences
WOS记录号WOS:000376211600015
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21073
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Fan, Jinxin,Luo, Jianquan,Chen, Xiangrong,et al. Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1448(MAY):121-126.
APA Fan, Jinxin,Luo, Jianquan,Chen, Xiangrong,&Wan, Yinhua.(2016).Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers.JOURNAL OF CHROMATOGRAPHY A,1448(MAY),121-126.
MLA Fan, Jinxin,et al."Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers".JOURNAL OF CHROMATOGRAPHY A 1448.MAY(2016):121-126.
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