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Polarity, selectivity and performance of hydrophilic organic/salt-containing aqueous two-phase system on counter-current chromatography for polar compounds
Liu, Dan1; Hong, Zhilai1; Gao, Mingzhe1; Wang, Zhixin1; Gu, Ming2; Zhang, Xiaozhe1; Xiao, Hongbin1,3
2016-05-27
发表期刊JOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
卷号1448期号:MAY页码:49-57
摘要The essential attributes of a solvent system for separation polar compounds on CCC are polarity, selectively and performance. Here, hydrophilic organic/salt-containing aqueous two-phase system (HO/S TPS) was evaluated as an alternative solvent system for CCC separation of polar compounds. Polarity measurements based on Rohrschneider-Snyder parameter was developed as quantitative assessing the polarity of HO/S TPS and comparing with an organic/aqueous system. All investigated 1-butanol/ethanol/saturated ammonium sulfate solution/water (BEAsWat) and 1-butanol/ethanol/saturated dipotassium hydrogen phosphate solution/water (BEDhpWat) systems with polarity values of organic phase from 4.5 to 6.8, were more polar than chloroform/methanol/water (1/1/1). The considerable water content of BEAsWat and BEDhpWat (0/1/1/1/) was 45.4 and 42.6% (w%) of hydrophilic organic phase, and 66.4 and 51.2% (w%) of salt-containing aqueous phase, respectively, closed to conventional aqueous two-phase system. Therefore, the polarity of HO/S TPS is in the middle of organic/aqueous and aqueous two-phase system. The LogK(C) values of twenty four polar compounds as model mixture confirmed that the polarities of HO/S TPSs were matched to that of the polar compounds and shown to be a very selective technique capable of separating positional isomers. Moreover, BEAsWat and BEDhpWat systems can be easily retained in CCC column with suitable elution mode. The hydrodynamic behavior reversion of HO/S TPS on hydrodynamic CCC was observed and was tentatively explained based on the density difference. Finally, caffeoylquinic acid isomers and dihydroxybenzoic acid isomers were successfully separated with HO/S TPS on CCC, respectively. Those results demonstrate that HO/S TPS on CCC is a performant and stable way to separate polar compounds from natural products. (C) 2016 Elsevier B.V. All rights reserved.
关键词Hydrophilic Organic/salt-containing Aqueous Two-phase System Counter-current Chromatography Hydrophilic Compounds Polarity Selectivity
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.chroma.2016.04.031
收录类别SCI
语种英语
关键词[WOS]FERMENTATION BROTHS ; SOLVENT SYSTEMS ; SALVIA-MILTIORRHIZA ; ACID-B ; SEPARATION ; PURIFICATION ; EXTRACTION ; 1,3-PROPANEDIOL ; 2,3-BUTANEDIOL ; PARTITION
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
项目资助者Natural Science Foundation of China(81303154) ; National Science and Technology Major Projects for "Major New Drugs Innovation and Development"(2014ZX09304307001-006)
WOS记录号WOS:000376211600006
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21086
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
3.Beijing Univ Chinese Med, Beijing, Peoples R China
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Liu, Dan,Hong, Zhilai,Gao, Mingzhe,et al. Polarity, selectivity and performance of hydrophilic organic/salt-containing aqueous two-phase system on counter-current chromatography for polar compounds[J]. JOURNAL OF CHROMATOGRAPHY A,2016,1448(MAY):49-57.
APA Liu, Dan.,Hong, Zhilai.,Gao, Mingzhe.,Wang, Zhixin.,Gu, Ming.,...&Xiao, Hongbin.(2016).Polarity, selectivity and performance of hydrophilic organic/salt-containing aqueous two-phase system on counter-current chromatography for polar compounds.JOURNAL OF CHROMATOGRAPHY A,1448(MAY),49-57.
MLA Liu, Dan,et al."Polarity, selectivity and performance of hydrophilic organic/salt-containing aqueous two-phase system on counter-current chromatography for polar compounds".JOURNAL OF CHROMATOGRAPHY A 1448.MAY(2016):49-57.
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