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Asymmetrical flow field-flow fractionation coupled with multi-angle laser light scattering for stability comparison of virus-like particles in different solution environments
Chen, Yi1,2; Zhang, Yan1; Zhou, Yuefang1; Luo, Jian1; Su, Zhiguo1,3
2016-06-08
发表期刊VACCINE
ISSN0264-410X
卷号34期号:27页码:3164-3170
摘要The stabilities of two commercially available virus like particles, CHO-HBsAg expressed by Chinese hamster ovary (CHO) cells and Hans-HBsAg expressed by Hansenula polymorpha (Hans), were compared using AF4-MALLS under different treatment processes. The initial molecular weight and hydrodynamic diameter of CHO-HBsAg measured with AF4-MALLS were 4727 kDa and 29.4nm, while those of Hans-HBsAg were 3039 kDa and 22.8 nm respectively. In salt solution of 2 M ammonium sulfate, the molecular weight and size of CHO-HBsAg had little change, and its antigenicity remained 95%, while those of Hans-HBsAg changed greatly, resulting in aggregation and 75% antigenicity loss. In freeze-thaw operations, Hans-HBsAg aggregated heavily. Most of the aggregates precipitated and the rest soluble aggregates reached 10(5)-10(6) kDa in molecular weight. The antigenicity of Hatis-HBsAg decreased to 26.9% after five freeze-thaw cycles. For CHO-HBsAg, there was no obvious aggregation in freeze-thaw, and the antigenicity retained above 98%. In heating process, Hans-HBsAg gradually aggregated to large particles with temperature and the antigenicity decreased to 10% when the temperature reached 80 degrees C. In contrast, CHO-HBsAg would not aggregate with temperature, remained 92% antigenicity at 80 degrees C. The study demonstrated that CHO-HBsAg appeared to be a superior vaccine antigen in term of particle stability and constant antigenicity, which are important in production, transportation and storage. (C) 2016 Elsevier Ltd. All rights reserved.
关键词Asymmetrical Flow field-Flow Fractionation Virus-like Particle Antigenicity Hepatitis b Surface Antigen Aggregation
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.vaccine.2016.04.046
收录类别SCI
语种英语
关键词[WOS]B SURFACE-ANTIGEN ; VACCINE FORMULATION ; SIZE ; IMMUNOGENICITY ; STABILIZATION ; AGGREGATION ; YEAST ; CELLS
WOS研究方向Immunology ; Research & Experimental Medicine
WOS类目Immunology ; Medicine, Research & Experimental
项目资助者Natural Sciences Foundation of China(21336010 ; National Key Basic Research Program of China(2013CB733600) ; 21306207)
WOS记录号WOS:000378667900023
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21172
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beiertiao 1, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing 210023, Jiangsu, Peoples R China
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Chen, Yi,Zhang, Yan,Zhou, Yuefang,et al. Asymmetrical flow field-flow fractionation coupled with multi-angle laser light scattering for stability comparison of virus-like particles in different solution environments[J]. VACCINE,2016,34(27):3164-3170.
APA Chen, Yi,Zhang, Yan,Zhou, Yuefang,Luo, Jian,&Su, Zhiguo.(2016).Asymmetrical flow field-flow fractionation coupled with multi-angle laser light scattering for stability comparison of virus-like particles in different solution environments.VACCINE,34(27),3164-3170.
MLA Chen, Yi,et al."Asymmetrical flow field-flow fractionation coupled with multi-angle laser light scattering for stability comparison of virus-like particles in different solution environments".VACCINE 34.27(2016):3164-3170.
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