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Scale-up laccase production from Trametes versicolor stimulated by vanillic acid
Wang, Ke-Feng1,2; Hu, Jian-Hua1,2; Guo, Chen1,2; Liu, Chun-Zhao1
2016-07-01
发表期刊BIOPROCESS AND BIOSYSTEMS ENGINEERING
ISSN1615-7591
卷号39期号:7页码:1041-1049
摘要An efficient strategy for laccase production in Trametes versicolor cultures was developed using vanillic acid as the inducer. The optimized vanillic acid treatment strategy consisted of exposing 2-day-old mycelia cultures to 80 mg/L vanillic acid. After 4 days, laccase activity of 588.84 U/L was achieved in flasks which represented a 1.79-fold increase compared to the control. In 200-L airlift bioreactor, the maximal laccase activity reached up to 785.12 U/L using the optimized vanillic acid treatment strategy. The zymograms of culture supernatants revealed three bands with laccase activity, among which Lac1 and Lac2 were abundant laccase isoforms constitutively expressed, and Lac3 was an inducible isozyme by vanillic acid. The results of real-time quantitative PCR showed that the transcription level of lcc in T. versicolor cultures grown with vanillic acid for 7 days was about 5.64-fold greater than that without vanillic acid in flasks. In 200-L airlift bioreactor cultures of T. versicolor with addition of vanillic acid, the transcript level of lcc at day 7 was 2.62-fold higher than that in flasks with vanillic acid due to the good mass transfer and oxygen supply in the bioreactor system. This study provides a basis for understanding the induction mechanism of vanillic acid for laccase production and has good potential for industrial applications.
关键词Trametes Versicolor Vanillic Acid Scale-up Culture Laccase Isoenzymes Lcc
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1007/s00449-016-1582-0
收录类别SCI
语种英语
关键词[WOS]PLEUROTUS-SAJOR-CAJU ; GENE-EXPRESSION ; AROMATIC-COMPOUNDS ; DIFFERENTIAL REGULATION ; SUBMERGED CULTURES ; FUNGI ; DECOLORIZATION ; OPTIMIZATION ; INDUCTION ; DYES
WOS研究方向Biotechnology & Applied Microbiology ; Engineering
WOS类目Biotechnology & Applied Microbiology ; Engineering, Chemical
项目资助者National Basic Research Program (973 Program) of China(2013CB733600) ; National Natural Science Foundation of China(21476242) ; National Key Technology Research and Development Program of China(2015BAK45B01) ; National High Technology Research and Development Program (863 Program) of China(2012AA101803) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KSZD -EW-Z-015) ; Chinese Academy of Sciences Fellowships for Young International Scientists(2015VMC060)
WOS记录号WOS:000378566700003
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21178
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wang, Ke-Feng,Hu, Jian-Hua,Guo, Chen,et al. Scale-up laccase production from Trametes versicolor stimulated by vanillic acid[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2016,39(7):1041-1049.
APA Wang, Ke-Feng,Hu, Jian-Hua,Guo, Chen,&Liu, Chun-Zhao.(2016).Scale-up laccase production from Trametes versicolor stimulated by vanillic acid.BIOPROCESS AND BIOSYSTEMS ENGINEERING,39(7),1041-1049.
MLA Wang, Ke-Feng,et al."Scale-up laccase production from Trametes versicolor stimulated by vanillic acid".BIOPROCESS AND BIOSYSTEMS ENGINEERING 39.7(2016):1041-1049.
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