| Scale-up laccase production from Trametes versicolor stimulated by vanillic acid | |
| Wang, Ke-Feng1,2; Hu, Jian-Hua1,2; Guo, Chen1,2; Liu, Chun-Zhao1 | |
| 2016-07-01 | |
| 发表期刊 | BIOPROCESS AND BIOSYSTEMS ENGINEERING
 |
| ISSN | 1615-7591 |
| 卷号 | 39期号:7页码:1041-1049 |
| 摘要 | An efficient strategy for laccase production in Trametes versicolor cultures was developed using vanillic acid as the inducer. The optimized vanillic acid treatment strategy consisted of exposing 2-day-old mycelia cultures to 80 mg/L vanillic acid. After 4 days, laccase activity of 588.84 U/L was achieved in flasks which represented a 1.79-fold increase compared to the control. In 200-L airlift bioreactor, the maximal laccase activity reached up to 785.12 U/L using the optimized vanillic acid treatment strategy. The zymograms of culture supernatants revealed three bands with laccase activity, among which Lac1 and Lac2 were abundant laccase isoforms constitutively expressed, and Lac3 was an inducible isozyme by vanillic acid. The results of real-time quantitative PCR showed that the transcription level of lcc in T. versicolor cultures grown with vanillic acid for 7 days was about 5.64-fold greater than that without vanillic acid in flasks. In 200-L airlift bioreactor cultures of T. versicolor with addition of vanillic acid, the transcript level of lcc at day 7 was 2.62-fold higher than that in flasks with vanillic acid due to the good mass transfer and oxygen supply in the bioreactor system. This study provides a basis for understanding the induction mechanism of vanillic acid for laccase production and has good potential for industrial applications. |
| 关键词 | Trametes Versicolor Vanillic Acid Scale-up Culture Laccase Isoenzymes Lcc |
| 文章类型 | Article |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| DOI | 10.1007/s00449-016-1582-0 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | PLEUROTUS-SAJOR-CAJU ; GENE-EXPRESSION ; AROMATIC-COMPOUNDS ; DIFFERENTIAL REGULATION ; SUBMERGED CULTURES ; FUNGI ; DECOLORIZATION ; OPTIMIZATION ; INDUCTION ; DYES |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering |
| WOS类目 | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 项目资助者 | National Basic Research Program (973 Program) of China(2013CB733600) ; National Natural Science Foundation of China(21476242) ; National Key Technology Research and Development Program of China(2015BAK45B01) ; National High Technology Research and Development Program (863 Program) of China(2012AA101803) ; Knowledge Innovation Program of the Chinese Academy of Sciences(KSZD -EW-Z-015) ; Chinese Academy of Sciences Fellowships for Young International Scientists(2015VMC060) |
| WOS记录号 | WOS:000378566700003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ipe.ac.cn/handle/122111/21178 |
| 专题 | 生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Ke-Feng,Hu, Jian-Hua,Guo, Chen,et al. Scale-up laccase production from Trametes versicolor stimulated by vanillic acid[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2016,39(7):1041-1049. |
| APA | Wang, Ke-Feng,Hu, Jian-Hua,Guo, Chen,&Liu, Chun-Zhao.(2016).Scale-up laccase production from Trametes versicolor stimulated by vanillic acid.BIOPROCESS AND BIOSYSTEMS ENGINEERING,39(7),1041-1049. |
| MLA | Wang, Ke-Feng,et al."Scale-up laccase production from Trametes versicolor stimulated by vanillic acid".BIOPROCESS AND BIOSYSTEMS ENGINEERING 39.7(2016):1041-1049. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Scale-up laccase pro（510KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论