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Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase
Zhu, Benwei1,2; Chen, Meijuan1; Yin, Heng3; Du, Yuguang4; Ning, Limin1
2016-06-01
发表期刊MARINE DRUGS
ISSN1660-3397
卷号14期号:6页码:108
摘要Enzymatic hydrolysis of sodium alginate to produce alginate oligosaccharides has drawn increasing attention due to its advantages of containing a wild reaction condition, excellent gel properties and specific products easy for purification. However, the efficient commercial enzyme tools are rarely available. A new alginate lyase with high activity (24,038 U/mg) has been purified from a newly isolated marine strain, Cellulophaga sp. NJ-1. The enzyme was most active at 50 degrees C and pH 8.0 and maintained stability at a broad pH range (6.0-10.0) and temperature below 40 degrees C. It had broad substrate specificity toward sodium alginate, heteropolymeric MG blocks (polyMG), homopolymeric M blocks (polyM) and homopolymeric G blocks (polyG), and possessed higher affinity toward polyG (15.63 mM) as well as polyMG (23.90 mM) than polyM (53.61 mM) and sodium alginate (27.21 mM). The TLC and MS spectroscopy analysis of degradation products suggested that it completely hydrolyzed sodium alginate into oligosaccharides of low degrees of polymerization (DPs). The excellent properties would make it a promising tool for full use of sodium alginate to produce oligosaccharides.
关键词Alginate Lyase Cellulophaga Enzymatic Hydrolysis Oligosaccharides
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.3390/md14060108
收录类别SCI
语种英语
关键词[WOS]OLIGOALGINATE LYASE ; CYTOKINE PRODUCTION ; DEGRADING ENZYMES ; PURIFICATION ; MACROPHAGES ; EXPRESSION ; INDUCTION ; CLONING ; CELLS
WOS研究方向Pharmacology & Pharmacy
WOS类目Chemistry, Medicinal
项目资助者Natural Science Foundation of the Jiangsu Higher Education Institutions of China(15KJB360007) ; National Natural Science Foundation of China(81503463)
WOS记录号WOS:000378770300005
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21179
专题研究所(批量导入)
作者单位1.Nanjing Univ Chinese Med, Preclin Med Coll, Nanjing 210023, Jiangsu, Peoples R China
2.Nanjing Univ Technol, Coll Food Sci & Light Ind, Nanjing 211816, Jiangsu, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
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Zhu, Benwei,Chen, Meijuan,Yin, Heng,et al. Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase[J]. MARINE DRUGS,2016,14(6):108.
APA Zhu, Benwei,Chen, Meijuan,Yin, Heng,Du, Yuguang,&Ning, Limin.(2016).Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase.MARINE DRUGS,14(6),108.
MLA Zhu, Benwei,et al."Enzymatic Hydrolysis of Alginate to Produce Oligosaccharides by a New Purified Endo-Type Alginate Lyase".MARINE DRUGS 14.6(2016):108.
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