Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry

doi:10.1002/elps.201500471

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	Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry
	Cheng, Likun 1,2; Xu, Qingsong 1; Yang, Kaiyun 3; He, Jun 3; Chen, Daiwen 2; Du, Yuguang 4; Yin, Heng 1
	2016-06-01
发表期刊	ELECTROPHORESIS
ISSN	0173-0835
卷号	37 期号:11 页码:1525-1531
摘要	Swine plays a significant role in livestock agriculture. As a linkage between sows and piglets, porcine milk is crucial for the health of newborn piglets. Free milk oligosaccharides (MOs) are kinds of important bioactive substance in mammalian milk. However, little is known about the component and function of the porcine MOs (PMOs). In this study, a hydrophilic interaction chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HILIC-Q-TOF MS) system was utilized to profile the PMOs. Forty-one distinct PMOs were identified totally in three breeds of sows. The PMOs were highly sialylated (similar to 30%) and fucosylated PMOs (1-3%) were monitored at low levels. The most abundant oligosaccharide was a trisaccharide (Hex(3)) which contributed over 50% of the total PMOs abundance. Comparison of free MOs profiles revealed heterogeneity and variations among individuals and different breeds of sows, however, the MOs variation among breeds was limited even minor than that among individuals. Furthermore, most PMOs contents were higher in colostrum and decreased in the early lactation, but a few kinds increased at last. Different oligosaccharides had different patterns during lactation. Overall, these observations showed a more detailed PMOs library and would contribute to the exploration of influence of PMOs on piglets' health.
关键词	Hydrophilic Interaction Chromatography Liquid Chromatography-mass Spectrometry Mass Spectrometry Porcine Milk Oligosaccharides
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1002/elps.201500471
收录类别	SCI
语种	英语
关键词[WOS]	BOVINE-MILK ; MICROBIOTA ; QUANTIFICATION ; GLYCANS ; GLYCOME
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
项目资助者	National Natural Science Foundation of China(31372347) ; Special Fund for Marine Scientific Research(201305015-2) ; National High Technology Research and Development Program of China (863 Program)(2014AA093604) ; National Key Science & Technology Support Program(2013BAB01B01) ; CAS Youth Innovation Promotion Association(2015144)
WOS记录号	WOS:000379135200015
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ipe.ac.cn/handle/122111/21190
专题	研究所（批量导入）
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Sichuan Agr Univ, Inst Anim Nutr, Chengdu, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Likun,Xu, Qingsong,Yang, Kaiyun,et al. Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry[J]. ELECTROPHORESIS,2016,37(11):1525-1531.
APA	Cheng, Likun.,Xu, Qingsong.,Yang, Kaiyun.,He, Jun.,Chen, Daiwen.,...&Yin, Heng.(2016).Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry.ELECTROPHORESIS,37(11),1525-1531.
MLA	Cheng, Likun,et al."Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry".ELECTROPHORESIS 37.11(2016):1525-1531.

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