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Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry
Cheng, Likun1,2; Xu, Qingsong1; Yang, Kaiyun3; He, Jun3; Chen, Daiwen2; Du, Yuguang4; Yin, Heng1
2016-06-01
发表期刊ELECTROPHORESIS
ISSN0173-0835
卷号37期号:11页码:1525-1531
摘要Swine plays a significant role in livestock agriculture. As a linkage between sows and piglets, porcine milk is crucial for the health of newborn piglets. Free milk oligosaccharides (MOs) are kinds of important bioactive substance in mammalian milk. However, little is known about the component and function of the porcine MOs (PMOs). In this study, a hydrophilic interaction chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HILIC-Q-TOF MS) system was utilized to profile the PMOs. Forty-one distinct PMOs were identified totally in three breeds of sows. The PMOs were highly sialylated (similar to 30%) and fucosylated PMOs (1-3%) were monitored at low levels. The most abundant oligosaccharide was a trisaccharide (Hex(3)) which contributed over 50% of the total PMOs abundance. Comparison of free MOs profiles revealed heterogeneity and variations among individuals and different breeds of sows, however, the MOs variation among breeds was limited even minor than that among individuals. Furthermore, most PMOs contents were higher in colostrum and decreased in the early lactation, but a few kinds increased at last. Different oligosaccharides had different patterns during lactation. Overall, these observations showed a more detailed PMOs library and would contribute to the exploration of influence of PMOs on piglets' health.
关键词Hydrophilic Interaction Chromatography Liquid Chromatography-mass Spectrometry Mass Spectrometry Porcine Milk Oligosaccharides
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1002/elps.201500471
收录类别SCI
语种英语
关键词[WOS]BOVINE-MILK ; MICROBIOTA ; QUANTIFICATION ; GLYCANS ; GLYCOME
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
项目资助者National Natural Science Foundation of China(31372347) ; Special Fund for Marine Scientific Research(201305015-2) ; National High Technology Research and Development Program of China (863 Program)(2014AA093604) ; National Key Science & Technology Support Program(2013BAB01B01) ; CAS Youth Innovation Promotion Association(2015144)
WOS记录号WOS:000379135200015
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21190
专题研究所(批量导入)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Sichuan Agr Univ, Inst Anim Nutr, Chengdu, Peoples R China
4.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China
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GB/T 7714
Cheng, Likun,Xu, Qingsong,Yang, Kaiyun,et al. Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry[J]. ELECTROPHORESIS,2016,37(11):1525-1531.
APA Cheng, Likun.,Xu, Qingsong.,Yang, Kaiyun.,He, Jun.,Chen, Daiwen.,...&Yin, Heng.(2016).Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry.ELECTROPHORESIS,37(11),1525-1531.
MLA Cheng, Likun,et al."Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry".ELECTROPHORESIS 37.11(2016):1525-1531.
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