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| Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry | |
| Cheng, Likun1,2; Xu, Qingsong1; Yang, Kaiyun3; He, Jun3; Chen, Daiwen2; Du, Yuguang4; Yin, Heng1 | |
| 2016-06-01 | |
| Source Publication | ELECTROPHORESIS
 |
| ISSN | 0173-0835 |
| Volume | 37Issue:11Pages:1525-1531 |
| Abstract | Swine plays a significant role in livestock agriculture. As a linkage between sows and piglets, porcine milk is crucial for the health of newborn piglets. Free milk oligosaccharides (MOs) are kinds of important bioactive substance in mammalian milk. However, little is known about the component and function of the porcine MOs (PMOs). In this study, a hydrophilic interaction chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (HILIC-Q-TOF MS) system was utilized to profile the PMOs. Forty-one distinct PMOs were identified totally in three breeds of sows. The PMOs were highly sialylated (similar to 30%) and fucosylated PMOs (1-3%) were monitored at low levels. The most abundant oligosaccharide was a trisaccharide (Hex(3)) which contributed over 50% of the total PMOs abundance. Comparison of free MOs profiles revealed heterogeneity and variations among individuals and different breeds of sows, however, the MOs variation among breeds was limited even minor than that among individuals. Furthermore, most PMOs contents were higher in colostrum and decreased in the early lactation, but a few kinds increased at last. Different oligosaccharides had different patterns during lactation. Overall, these observations showed a more detailed PMOs library and would contribute to the exploration of influence of PMOs on piglets' health. |
| Keyword | Hydrophilic Interaction Chromatography Liquid Chromatography-mass Spectrometry Mass Spectrometry Porcine Milk Oligosaccharides |
| Subtype | Article |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1002/elps.201500471 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | BOVINE-MILK ; MICROBIOTA ; QUANTIFICATION ; GLYCANS ; GLYCOME |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemical Research Methods ; Chemistry, Analytical |
| Funding Organization | National Natural Science Foundation of China(31372347) ; Special Fund for Marine Scientific Research(201305015-2) ; National High Technology Research and Development Program of China (863 Program)(2014AA093604) ; National Key Science & Technology Support Program(2013BAB01B01) ; CAS Youth Innovation Promotion Association(2015144) |
| WOS ID | WOS:000379135200015 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ipe.ac.cn/handle/122111/21190 |
| Collection | 研究所（批量导入） |
| Affiliation | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Sichuan Agr Univ, Inst Anim Nutr, Chengdu, Peoples R China 4.Chinese Acad Sci, Inst Proc Engn, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Cheng, Likun,Xu, Qingsong,Yang, Kaiyun,et al. Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry[J]. ELECTROPHORESIS,2016,37(11):1525-1531. |
| APA | Cheng, Likun.,Xu, Qingsong.,Yang, Kaiyun.,He, Jun.,Chen, Daiwen.,...&Yin, Heng.(2016).Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry.ELECTROPHORESIS,37(11),1525-1531. |
| MLA | Cheng, Likun,et al."Annotation of porcine milk oligosaccharides throughout lactation by hydrophilic interaction chromatography coupled with quadruple time of flight tandem mass spectrometry".ELECTROPHORESIS 37.11(2016):1525-1531. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Cheng_et_al-2016-ELE（505KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text | |
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