CAS OpenIR  > 生化工程国家重点实验室
Effects of Impurities in Alkali-Extracted Xylan on Its Enzymatic Hydrolysis to Produce Xylo-Oligosaccharides
Shen, Rui1,2; Li, Hong-Qiang1; Zhang, Jie2; Xu, Jian1
2016-07-01
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
Volume179Issue:5Pages:740-752
AbstractAs the second abundant natural carbohydrate, xylan is normally prepared through alkaline extraction and then used for xylo-oligosaccharides (XOS) production. However, the extracted xylan inevitably contains salt, ethanol, and pigment. In order to investigate the effects of these impurities on XOS production, the alkaline-extracted xylan with different kinds and concentrations of impurities was made and then hydrolyzed using alkaline xylanase (EC 3.2.1.8) to produce XOS. The results showed that a certain concentration of salt (NaCl) promoted the XOS production, while ethanol and pigment inhibited the enzymatic hydrolysis process significantly. The color value mainly ascribed to the phenolic compounds binding to xylan was a key restriction factor in the enzymatic hydrolysis later stage. Using optimal xylan sample (with 10 mg/mL NaCl, color value of 4.6 x 10(5), without ethanol) as substrate, the highest XOS yield of 58.58 % was obtained. As the substrate of XOS production, prepared xylan should contain colored materials and ethanol as less as possible, however, retains appropriate salt.
KeywordXylan Enzymatic Hydrolysis Xylo-oligosaccharides Sodium Chloride Ethanol Pigment
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s12010-016-2028-5
Indexed BySCI
Language英语
WOS KeywordLIGNOCELLULOSIC BIOMASS ; XYLOOLIGOSACCHARIDES ; PURIFICATION ; MANUFACTURE ; CELLULOSE
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
Funding OrganizationNational Natural Science Foundation of China(21276259) ; NSFC international (regional) cooperation and exchange project: the International Fund for Young Scientists(21450110062)
WOS IDWOS:000379755800005
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21232
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Beijing Univ Chem Technol, Beijing 100029, Peoples R China
Recommended Citation
GB/T 7714
Shen, Rui,Li, Hong-Qiang,Zhang, Jie,et al. Effects of Impurities in Alkali-Extracted Xylan on Its Enzymatic Hydrolysis to Produce Xylo-Oligosaccharides[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2016,179(5):740-752.
APA Shen, Rui,Li, Hong-Qiang,Zhang, Jie,&Xu, Jian.(2016).Effects of Impurities in Alkali-Extracted Xylan on Its Enzymatic Hydrolysis to Produce Xylo-Oligosaccharides.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,179(5),740-752.
MLA Shen, Rui,et al."Effects of Impurities in Alkali-Extracted Xylan on Its Enzymatic Hydrolysis to Produce Xylo-Oligosaccharides".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 179.5(2016):740-752.
Files in This Item:
File Name/Size DocType Version Access License
Effects of Impuritie(1113KB)期刊论文出版稿限制开放CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Shen, Rui]'s Articles
[Li, Hong-Qiang]'s Articles
[Zhang, Jie]'s Articles
Baidu academic
Similar articles in Baidu academic
[Shen, Rui]'s Articles
[Li, Hong-Qiang]'s Articles
[Zhang, Jie]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Shen, Rui]'s Articles
[Li, Hong-Qiang]'s Articles
[Zhang, Jie]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.