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Molecular weight controllable degradation of Laminaria japonica polysaccharides and its antioxidant properties
Zha Shenghua1,2; Zhao Qingsheng1; Zhao Bing1; Ouyang Jie3; Mo Jianling3; Chen Jinjin1; Cao Lili1; Zhang Hong4
2016-08-01
Source PublicationJOURNAL OF OCEAN UNIVERSITY OF CHINA
ISSN1672-5182
Volume15Issue:4Pages:637-642
AbstractIn this study, molecular weight controllable degradation of algal Laminaria japonica polysaccharides (LPS) was investigated by ultrasound combined with hydrogen peroxide. Three main factors, i.e., ultrasonic power (A), ultrasonic time (B), and H2O2 concentration (C) were chosen for optimizing parameters by employing three-factors, three-levels BBD. The influence of degradation on structure change and antioxidant activities was also investigated. A second-order polynomial equation including molecular weight (Y) of Laminaria japonica polysaccharides and each variable parameter, i.e., ultrasonic power (A), ultrasonic time (B), and H2O2 concentration (C), was established: Y=20718.67-4273.13A-4000.38B-1438.75C+2333.25AB+1511.00AC+873.00BC+2838.29A (2) + 2490.79B (2)+873.04C (2). The equation regression coefficient value (R (2) = 0.969) indicated that this equation was valid. The value of the adjusted determination coefficient (adjusted R (2) = 0.914) also confirmed that the model was highly significant. The results of selected experimental degradation conditions matched with the predicted value. FT-IR spectra revealed that the structures of LPS before and after degradation were not significantly changed. Antioxidant activities of LPS revealed that low Mws possessed stronger inhibitory than the original polysaccharides. The scavenging effects on superoxide radicals was the highest when IC50 of crude LPS was 4.92 mg mL(-1) and IC50 of Mw 18.576 KDa was 1.02 mg mL(-1), which was fourfold higher than initial polysaccharide.
KeywordLaminaria Japonica Polysaccharides Degradation Ultrasound
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1007/s11802-016-2943-7
Indexed BySCI
Language英语
WOS KeywordSULFATED POLYSACCHARIDES ; CHLOROPHYTA
WOS Research AreaOceanography
WOS SubjectOceanography
Funding OrganizationNational Natural Science Foundation of China(21506220)
WOS IDWOS:000379536300009
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21251
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China
4.Beijing Tong Ren Tang Hlth Pharmaceut Co Ltd, Beijing 100085, Peoples R China
Recommended Citation
GB/T 7714
Zha Shenghua,Zhao Qingsheng,Zhao Bing,et al. Molecular weight controllable degradation of Laminaria japonica polysaccharides and its antioxidant properties[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2016,15(4):637-642.
APA Zha Shenghua.,Zhao Qingsheng.,Zhao Bing.,Ouyang Jie.,Mo Jianling.,...&Zhang Hong.(2016).Molecular weight controllable degradation of Laminaria japonica polysaccharides and its antioxidant properties.JOURNAL OF OCEAN UNIVERSITY OF CHINA,15(4),637-642.
MLA Zha Shenghua,et al."Molecular weight controllable degradation of Laminaria japonica polysaccharides and its antioxidant properties".JOURNAL OF OCEAN UNIVERSITY OF CHINA 15.4(2016):637-642.
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