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High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism
Zhang, Bo1,4; Sun, Hongbing1,2; Li, Jing3; Wan, Yinhua3; Li, Yin1; Zhang, Yanping1
2016-08-01
Source PublicationPROCESS BIOCHEMISTRY
ISSN1359-5113
Volume51Issue:8Pages:967-972
AbstractEfficient xylose utilization is critical for the production of fuels from biomass hydrolysates. It is known that xylose catabolism is inhibited by glucose. In this study, we showed that ethanol also inhibits xylose catabolism. By introducing a xylose metabolic pathway into Saccharomyces cerevisiae and using evolutionary engineering, an engineered S. cerevisiae strain, W32N55, was obtained that can anaerobically ferment xylose to ethanol. The effect of ethanol on xylose utilization was investigated. The results showed that xylose catabolism was inhibited upon the addition of ethanol, and it resumed once ethanol was removed. Based on these results, a fermentation-pervaporation coupling process was developed. After the in situ removal of ethanol, 150 g/L glucose and 31 g/L xylose were consumed in 72 h, providing a total of 76 g/L ethanol and an overall total sugar yield of 0.42 g/g. We believe that this strain will be valuable to the bio-ethanol industry. (C) 2016 Elsevier Ltd. All rights reserved.
KeywordCellulosic Ethanol Ethanol Inhibition Fermentation-pervaporation Saccharomyces Cerevisiae Xylose Catabolism
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.procbio.2016.04.019
Indexed BySCI
Language英语
WOS KeywordFERMENTATION ; GLUCOSE ; PERVAPORATION ; EXPRESSION ; GROWTH ; XYL1 ; XKS1
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNational High Technology Research and Development Program of China(2012AA022106 ; Knowledge Innovation Project of the Chinese Academy of Sciences(KSCX2-EW-Q-14 ; 2012AA101807) ; KSCX1-YW-11C3)
WOS IDWOS:000380972500005
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21285
Collection研究所(批量导入)
Affiliation1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
4.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Bo,Sun, Hongbing,Li, Jing,et al. High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism[J]. PROCESS BIOCHEMISTRY,2016,51(8):967-972.
APA Zhang, Bo,Sun, Hongbing,Li, Jing,Wan, Yinhua,Li, Yin,&Zhang, Yanping.(2016).High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism.PROCESS BIOCHEMISTRY,51(8),967-972.
MLA Zhang, Bo,et al."High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism".PROCESS BIOCHEMISTRY 51.8(2016):967-972.
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