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High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism
Zhang, Bo1,4; Sun, Hongbing1,2; Li, Jing3; Wan, Yinhua3; Li, Yin1; Zhang, Yanping1
2016-08-01
发表期刊PROCESS BIOCHEMISTRY
ISSN1359-5113
卷号51期号:8页码:967-972
摘要Efficient xylose utilization is critical for the production of fuels from biomass hydrolysates. It is known that xylose catabolism is inhibited by glucose. In this study, we showed that ethanol also inhibits xylose catabolism. By introducing a xylose metabolic pathway into Saccharomyces cerevisiae and using evolutionary engineering, an engineered S. cerevisiae strain, W32N55, was obtained that can anaerobically ferment xylose to ethanol. The effect of ethanol on xylose utilization was investigated. The results showed that xylose catabolism was inhibited upon the addition of ethanol, and it resumed once ethanol was removed. Based on these results, a fermentation-pervaporation coupling process was developed. After the in situ removal of ethanol, 150 g/L glucose and 31 g/L xylose were consumed in 72 h, providing a total of 76 g/L ethanol and an overall total sugar yield of 0.42 g/g. We believe that this strain will be valuable to the bio-ethanol industry. (C) 2016 Elsevier Ltd. All rights reserved.
关键词Cellulosic Ethanol Ethanol Inhibition Fermentation-pervaporation Saccharomyces Cerevisiae Xylose Catabolism
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.procbio.2016.04.019
收录类别SCI
语种英语
关键词[WOS]FERMENTATION ; GLUCOSE ; PERVAPORATION ; EXPRESSION ; GROWTH ; XYL1 ; XKS1
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS类目Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
项目资助者National High Technology Research and Development Program of China(2012AA022106 ; Knowledge Innovation Project of the Chinese Academy of Sciences(KSCX2-EW-Q-14 ; 2012AA101807) ; KSCX1-YW-11C3)
WOS记录号WOS:000380972500005
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21285
专题研究所(批量导入)
作者单位1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China
2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
4.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
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Zhang, Bo,Sun, Hongbing,Li, Jing,et al. High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism[J]. PROCESS BIOCHEMISTRY,2016,51(8):967-972.
APA Zhang, Bo,Sun, Hongbing,Li, Jing,Wan, Yinhua,Li, Yin,&Zhang, Yanping.(2016).High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism.PROCESS BIOCHEMISTRY,51(8),967-972.
MLA Zhang, Bo,et al."High-titer-ethanol production from cellulosic hydrolysate by an engineered strain of Saccharomyces cerevisiae during an in situ removal process reducing the inhibition of ethanol on xylose metabolism".PROCESS BIOCHEMISTRY 51.8(2016):967-972.
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