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Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain length
Bao, Luyao1,3; Li, Jian-Jun1,5; Jia, Chenjun3,4; Li, Mei4; Lu, Xuefeng2
2016-08-31
发表期刊BIOTECHNOLOGY FOR BIOFUELS
ISSN1754-6834
卷号9期号:AUG页码:185
摘要

Background: Aldehyde-deformylating oxygenase (ADO) is an important enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, ADO exhibits quite low chain-length specificity with respect to the substrates ranging from C-4 to C-18 aldehydes, which is not suitable for producing fuels with different properties or different chain lengths.

关键词Aldehyde-deformylating Oxygenase Site-directed Mutagenesis Structure-guided Protein Engineering Chain-length Selectivity Synechococcus Elongatus Pcc7942
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1186/s13068-016-0596-9
收录类别SCI
语种英语
关键词[WOS]CYANOBACTERIAL ALDEHYDE ; FATTY-ACIDS ; ESCHERICHIA-COLI ; CARRIER PROTEIN ; E. COLI ; CONVERSION ; ALKANES ; DECARBONYLASE ; OVERPRODUCTION ; BIOSYNTHESIS
WOS研究方向Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目Biotechnology & Applied Microbiology ; Energy & Fuels
项目资助者National Science Foundation of China(31170765 ; National Basic Research Program of China (973)(2011CBA00907 ; CAS Cross and Cooperation Team for ScientificInnovation(Y31102110A) ; Excellent Youth Award of the Shandong NaturalScience Foundation(JQ201306) ; Shandong Taishan Scholarship ; 31370799) ; 2011CBA00902)
WOS记录号WOS:000383016100003
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21317
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Key Lab Biofuels, Qingdao, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, 189 Songling Rd, Qingdao 266101, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, 15 Datun Rd, Beijing 100101, Peoples R China
5.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, 1 North 2nd St, Beijing 100190, Peoples R China
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GB/T 7714
Bao, Luyao,Li, Jian-Jun,Jia, Chenjun,et al. Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain length[J]. BIOTECHNOLOGY FOR BIOFUELS,2016,9(AUG):185.
APA Bao, Luyao,Li, Jian-Jun,Jia, Chenjun,Li, Mei,&Lu, Xuefeng.(2016).Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain length.BIOTECHNOLOGY FOR BIOFUELS,9(AUG),185.
MLA Bao, Luyao,et al."Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain length".BIOTECHNOLOGY FOR BIOFUELS 9.AUG(2016):185.
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