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The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine
Wang, Yue-Qi1,2; Liu, Yan1,2; Wang, Yu-Xia3; Wu, Ya-Jun3; Jia, Pei-Yuan1; Shan, Jun-Jie3; Wu, Jie1; Ma, Guang-Hui1,4; Su, Zhi-Guo1
2016-10-01
发表期刊INTERNATIONAL IMMUNOPHARMACOLOGY
ISSN1567-5769
卷号39期号:OCT页码:84-91
摘要

Infectious diseases possess a big threat to the livestock industry worldwide. Currently, inactivated veterinary vaccines have attracted much attention to prevent infection due to their safer profile compared to live attenuated vaccine. However, its intrinsic poor immunogenicity demands the incorporation of an adjuvant. Mineral oil based adjuvant (Montanide (TM) ISA206) was usually used to potentiate the efficacy of veterinary vaccines. However, ISA206 could not induce robust cellular immune responses, which was very important in controlling virus replication and clearing the infected cells. Moreover, mineral oil would result in severe side effects. To improve both the humoral and cellular immune responses of porcine reproductive and respiratory syndrome virus (PRRSV) inactivated vaccine, we developed pH-sensitive and size-controllable quaternized chitosan hydrogel microparticles (Gel MPs) without using chemical cross linking agent. Gel MPs, ionic cross-linked with glycerophosphate (GP), were biocompatible and could efficiently adsorb the inactivated PRRSV vaccine with a loading capacity of 579.05 mu g/mg. After intramuscular immunization in mice, results suggested that Gel MPs elicited significantly higher cell-mediated immune responses and comparable humoral immune responses compared to ISA 206. Re, garding the biocompatibility, safety and effectiveness, Gel MPs would be a promising candidate to enhance the efficacy of veterinary vaccine. (C) 2016 Elsevier B.V. All rights reserved.

关键词Quaternized Chitosan Hydrogel Microparticles Adjuvant Veterinary Vaccine Porcine Reproductive And Respiratory Syndrome Virus Isa 206
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.intimp.2016.07.012
收录类别SCI
语种英语
关键词[WOS]CLUSTER FIELD STRAIN ; OIL-BASED ADJUVANTS ; VETERINARY VACCINES ; IMMUNE-RESPONSES ; DELIVERY-SYSTEM ; PRRSV VACCINE ; NANOPARTICLES ; PIGS ; MICRONEEDLES ; IMMUNIZATION
WOS研究方向Immunology ; Pharmacology & Pharmacy
WOS类目Immunology ; Pharmacology & Pharmacy
项目资助者National Science and Technology Major Project(2014ZX09102045) ; National High TechnoloSgy Research, Development Program of China(2014AA093604) ; Special Fund for Agro-scientific Research in the public Interest(201303046)
WOS记录号WOS:000383932000012
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21333
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Acad Mil Med Sci, Inst Pharmacol & Toxicol, Beijing, Peoples R China
4.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing, Jiangsu, Peoples R China
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Wang, Yue-Qi,Liu, Yan,Wang, Yu-Xia,et al. The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2016,39(OCT):84-91.
APA Wang, Yue-Qi.,Liu, Yan.,Wang, Yu-Xia.,Wu, Ya-Jun.,Jia, Pei-Yuan.,...&Su, Zhi-Guo.(2016).The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine.INTERNATIONAL IMMUNOPHARMACOLOGY,39(OCT),84-91.
MLA Wang, Yue-Qi,et al."The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine".INTERNATIONAL IMMUNOPHARMACOLOGY 39.OCT(2016):84-91.
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