The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine | |
Wang, Yue-Qi1,2; Liu, Yan1,2; Wang, Yu-Xia3; Wu, Ya-Jun3; Jia, Pei-Yuan1; Shan, Jun-Jie3; Wu, Jie1; Ma, Guang-Hui1,4; Su, Zhi-Guo1 | |
2016-10-01 | |
Source Publication | INTERNATIONAL IMMUNOPHARMACOLOGY
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ISSN | 1567-5769 |
Volume | 39Issue:OCTPages:84-91 |
Abstract | Infectious diseases possess a big threat to the livestock industry worldwide. Currently, inactivated veterinary vaccines have attracted much attention to prevent infection due to their safer profile compared to live attenuated vaccine. However, its intrinsic poor immunogenicity demands the incorporation of an adjuvant. Mineral oil based adjuvant (Montanide (TM) ISA206) was usually used to potentiate the efficacy of veterinary vaccines. However, ISA206 could not induce robust cellular immune responses, which was very important in controlling virus replication and clearing the infected cells. Moreover, mineral oil would result in severe side effects. To improve both the humoral and cellular immune responses of porcine reproductive and respiratory syndrome virus (PRRSV) inactivated vaccine, we developed pH-sensitive and size-controllable quaternized chitosan hydrogel microparticles (Gel MPs) without using chemical cross linking agent. Gel MPs, ionic cross-linked with glycerophosphate (GP), were biocompatible and could efficiently adsorb the inactivated PRRSV vaccine with a loading capacity of 579.05 mu g/mg. After intramuscular immunization in mice, results suggested that Gel MPs elicited significantly higher cell-mediated immune responses and comparable humoral immune responses compared to ISA 206. Re, garding the biocompatibility, safety and effectiveness, Gel MPs would be a promising candidate to enhance the efficacy of veterinary vaccine. (C) 2016 Elsevier B.V. All rights reserved. |
Keyword | Quaternized Chitosan Hydrogel Microparticles Adjuvant Veterinary Vaccine Porcine Reproductive And Respiratory Syndrome Virus Isa 206 |
Subtype | Article |
WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1016/j.intimp.2016.07.012 |
Indexed By | SCI |
Language | 英语 |
WOS Keyword | CLUSTER FIELD STRAIN ; OIL-BASED ADJUVANTS ; VETERINARY VACCINES ; IMMUNE-RESPONSES ; DELIVERY-SYSTEM ; PRRSV VACCINE ; NANOPARTICLES ; PIGS ; MICRONEEDLES ; IMMUNIZATION |
WOS Research Area | Immunology ; Pharmacology & Pharmacy |
WOS Subject | Immunology ; Pharmacology & Pharmacy |
Funding Organization | National Science and Technology Major Project(2014ZX09102045) ; National High TechnoloSgy Research, Development Program of China(2014AA093604) ; Special Fund for Agro-scientific Research in the public Interest(201303046) |
WOS ID | WOS:000383932000012 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ipe.ac.cn/handle/122111/21333 |
Collection | 生化工程国家重点实验室 |
Affiliation | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Acad Mil Med Sci, Inst Pharmacol & Toxicol, Beijing, Peoples R China 4.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing, Jiangsu, Peoples R China |
Recommended Citation GB/T 7714 | Wang, Yue-Qi,Liu, Yan,Wang, Yu-Xia,et al. The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2016,39(OCT):84-91. |
APA | Wang, Yue-Qi.,Liu, Yan.,Wang, Yu-Xia.,Wu, Ya-Jun.,Jia, Pei-Yuan.,...&Su, Zhi-Guo.(2016).The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine.INTERNATIONAL IMMUNOPHARMACOLOGY,39(OCT),84-91. |
MLA | Wang, Yue-Qi,et al."The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine".INTERNATIONAL IMMUNOPHARMACOLOGY 39.OCT(2016):84-91. |
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