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The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine
Wang, Yue-Qi1,2; Liu, Yan1,2; Wang, Yu-Xia3; Wu, Ya-Jun3; Jia, Pei-Yuan1; Shan, Jun-Jie3; Wu, Jie1; Ma, Guang-Hui1,4; Su, Zhi-Guo1
2016-10-01
Source PublicationINTERNATIONAL IMMUNOPHARMACOLOGY
ISSN1567-5769
Volume39Issue:OCTPages:84-91
Abstract

Infectious diseases possess a big threat to the livestock industry worldwide. Currently, inactivated veterinary vaccines have attracted much attention to prevent infection due to their safer profile compared to live attenuated vaccine. However, its intrinsic poor immunogenicity demands the incorporation of an adjuvant. Mineral oil based adjuvant (Montanide (TM) ISA206) was usually used to potentiate the efficacy of veterinary vaccines. However, ISA206 could not induce robust cellular immune responses, which was very important in controlling virus replication and clearing the infected cells. Moreover, mineral oil would result in severe side effects. To improve both the humoral and cellular immune responses of porcine reproductive and respiratory syndrome virus (PRRSV) inactivated vaccine, we developed pH-sensitive and size-controllable quaternized chitosan hydrogel microparticles (Gel MPs) without using chemical cross linking agent. Gel MPs, ionic cross-linked with glycerophosphate (GP), were biocompatible and could efficiently adsorb the inactivated PRRSV vaccine with a loading capacity of 579.05 mu g/mg. After intramuscular immunization in mice, results suggested that Gel MPs elicited significantly higher cell-mediated immune responses and comparable humoral immune responses compared to ISA 206. Re, garding the biocompatibility, safety and effectiveness, Gel MPs would be a promising candidate to enhance the efficacy of veterinary vaccine. (C) 2016 Elsevier B.V. All rights reserved.

KeywordQuaternized Chitosan Hydrogel Microparticles Adjuvant Veterinary Vaccine Porcine Reproductive And Respiratory Syndrome Virus Isa 206
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.intimp.2016.07.012
Indexed BySCI
Language英语
WOS KeywordCLUSTER FIELD STRAIN ; OIL-BASED ADJUVANTS ; VETERINARY VACCINES ; IMMUNE-RESPONSES ; DELIVERY-SYSTEM ; PRRSV VACCINE ; NANOPARTICLES ; PIGS ; MICRONEEDLES ; IMMUNIZATION
WOS Research AreaImmunology ; Pharmacology & Pharmacy
WOS SubjectImmunology ; Pharmacology & Pharmacy
Funding OrganizationNational Science and Technology Major Project(2014ZX09102045) ; National High TechnoloSgy Research, Development Program of China(2014AA093604) ; Special Fund for Agro-scientific Research in the public Interest(201303046)
WOS IDWOS:000383932000012
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21333
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Acad Mil Med Sci, Inst Pharmacol & Toxicol, Beijing, Peoples R China
4.Jiangsu Natl Synerget Innovat Ctr Adv Mat SICAM, Nanjing, Jiangsu, Peoples R China
Recommended Citation
GB/T 7714
Wang, Yue-Qi,Liu, Yan,Wang, Yu-Xia,et al. The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2016,39(OCT):84-91.
APA Wang, Yue-Qi.,Liu, Yan.,Wang, Yu-Xia.,Wu, Ya-Jun.,Jia, Pei-Yuan.,...&Su, Zhi-Guo.(2016).The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine.INTERNATIONAL IMMUNOPHARMACOLOGY,39(OCT),84-91.
MLA Wang, Yue-Qi,et al."The potential adjuvanticity of quaternized chitosan hydrogel based microparticles for porcine reproductive and respiratory syndrome virus inactivated vaccine".INTERNATIONAL IMMUNOPHARMACOLOGY 39.OCT(2016):84-91.
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