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Fabrication of high-capacity cation-exchangers for protein chromatography by atom transfer radical polymerization
Wang, Hong-Yan1,2; Sun, Yan1,2; Zhang, Su-Ling1,2; Luo, Jian3; Shi, Qing-Hong1,2,3
2016-09-15
Source PublicationBIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
Volume113Issue:SEPPages:19-29
Abstract

This work reports the synthesis of novel cation-exchanger with controllable charge density and polymer chain length of poly(3-sulfopropyl methacrylate) (poly(SPM)) grafted via atom transfer radical polymerization onto Sepharose FF matrix. Polymer grafting provided a three-dimensional regular arrangement of the ligand and increased ionic capacity of the poly(SPM)-grafted cation-exchangers. The result showed that adsorption capacity for lysozyme enhanced greatly in poly(SPM)-grafted cation-exchangers whereas adsorption capacity for gamma-globulin decreased dramatically. It can be attributed to the consequence of the competition between electrostatic interaction and repulsive excluded volume interaction of grafted polymer and protein. For lysozyme, protein adsorption on poly(SPM)-grafted cation exchangers was driven dominantly by electrostatic interaction. By constrast, the repulsive excluded volume interaction between grafted polymer and protein was remarkable in adsorption of gamma-globulin on poly(SPM)-grafted cation exchangers. Moreover, the poly(SPM)-grafted cation exchangers exhibited great salt tolerance in protein adsorption and distinct intraparticle mass transfer properties. Finally, the results of dynamic binding capacity (DBC) for lysozyme showed that the poly(SPM)-grafted cation-exchangers had higher binding capacities than did the commercial SP Sepharose FF, and the maximal DBCs reached 192 mg/mL at 50 mmol/L NaCl. These results demonstrate great potential of poly(SPM)-grafted cation-exchangers for the large-scale purification of proteins. (C) 2016 Elsevier B.V. All rights reserved.

KeywordAdsorption Chromatography Polyclonal Antibodies Diffusion Atom Transfer Radical Polymerization High Capacity
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.bej.2016.05.006
Indexed BySCI
Language英语
WOS KeywordENTROPIC INTERACTION CHROMATOGRAPHY ; PORE-SIZE DISTRIBUTIONS ; ION-EXCHANGERS ; SEPHAROSE FF ; COMPOSITE MEDIA ; ADSORPTION ; TRANSPORT ; BRUSHES ; ADSORBENTS ; STRENGTH
WOS Research AreaBiotechnology & Applied Microbiology ; Engineering
WOS SubjectBiotechnology & Applied Microbiology ; Engineering, Chemical
Funding OrganizationNatural Science Foundation of China(21476166 ; High Tech Research and Development Program of China of the Ministry of Science and Technology of China(2012AA020206) ; National Key Laboratory of Biochemical Engineering(2014KF-03) ; 21236005)
WOS IDWOS:000380869600004
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21428
Collection生化工程国家重点实验室
Affiliation1.Tianjin Univ, Dept Biochem Engn, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China
2.Tianjin Univ, Key Lab Syst Bioengn, Sch Chem Engn & Technol, Minist Educ, Tianjin 300072, Peoples R China
3.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Wang, Hong-Yan,Sun, Yan,Zhang, Su-Ling,et al. Fabrication of high-capacity cation-exchangers for protein chromatography by atom transfer radical polymerization[J]. BIOCHEMICAL ENGINEERING JOURNAL,2016,113(SEP):19-29.
APA Wang, Hong-Yan,Sun, Yan,Zhang, Su-Ling,Luo, Jian,&Shi, Qing-Hong.(2016).Fabrication of high-capacity cation-exchangers for protein chromatography by atom transfer radical polymerization.BIOCHEMICAL ENGINEERING JOURNAL,113(SEP),19-29.
MLA Wang, Hong-Yan,et al."Fabrication of high-capacity cation-exchangers for protein chromatography by atom transfer radical polymerization".BIOCHEMICAL ENGINEERING JOURNAL 113.SEP(2016):19-29.
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