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Fabrication of high-capacity cation-exchangers for protein chromatography by atom transfer radical polymerization
Wang, Hong-Yan1,2; Sun, Yan1,2; Zhang, Su-Ling1,2; Luo, Jian3; Shi, Qing-Hong1,2,3
2016-09-15
发表期刊BIOCHEMICAL ENGINEERING JOURNAL
ISSN1369-703X
卷号113期号:SEP页码:19-29
摘要

This work reports the synthesis of novel cation-exchanger with controllable charge density and polymer chain length of poly(3-sulfopropyl methacrylate) (poly(SPM)) grafted via atom transfer radical polymerization onto Sepharose FF matrix. Polymer grafting provided a three-dimensional regular arrangement of the ligand and increased ionic capacity of the poly(SPM)-grafted cation-exchangers. The result showed that adsorption capacity for lysozyme enhanced greatly in poly(SPM)-grafted cation-exchangers whereas adsorption capacity for gamma-globulin decreased dramatically. It can be attributed to the consequence of the competition between electrostatic interaction and repulsive excluded volume interaction of grafted polymer and protein. For lysozyme, protein adsorption on poly(SPM)-grafted cation exchangers was driven dominantly by electrostatic interaction. By constrast, the repulsive excluded volume interaction between grafted polymer and protein was remarkable in adsorption of gamma-globulin on poly(SPM)-grafted cation exchangers. Moreover, the poly(SPM)-grafted cation exchangers exhibited great salt tolerance in protein adsorption and distinct intraparticle mass transfer properties. Finally, the results of dynamic binding capacity (DBC) for lysozyme showed that the poly(SPM)-grafted cation-exchangers had higher binding capacities than did the commercial SP Sepharose FF, and the maximal DBCs reached 192 mg/mL at 50 mmol/L NaCl. These results demonstrate great potential of poly(SPM)-grafted cation-exchangers for the large-scale purification of proteins. (C) 2016 Elsevier B.V. All rights reserved.

关键词Adsorption Chromatography Polyclonal Antibodies Diffusion Atom Transfer Radical Polymerization High Capacity
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1016/j.bej.2016.05.006
收录类别SCI
语种英语
关键词[WOS]ENTROPIC INTERACTION CHROMATOGRAPHY ; PORE-SIZE DISTRIBUTIONS ; ION-EXCHANGERS ; SEPHAROSE FF ; COMPOSITE MEDIA ; ADSORPTION ; TRANSPORT ; BRUSHES ; ADSORBENTS ; STRENGTH
WOS研究方向Biotechnology & Applied Microbiology ; Engineering
WOS类目Biotechnology & Applied Microbiology ; Engineering, Chemical
项目资助者Natural Science Foundation of China(21476166 ; High Tech Research and Development Program of China of the Ministry of Science and Technology of China(2012AA020206) ; National Key Laboratory of Biochemical Engineering(2014KF-03) ; 21236005)
WOS记录号WOS:000380869600004
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21428
专题生化工程国家重点实验室
作者单位1.Tianjin Univ, Dept Biochem Engn, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China
2.Tianjin Univ, Key Lab Syst Bioengn, Sch Chem Engn & Technol, Minist Educ, Tianjin 300072, Peoples R China
3.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China
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GB/T 7714
Wang, Hong-Yan,Sun, Yan,Zhang, Su-Ling,et al. Fabrication of high-capacity cation-exchangers for protein chromatography by atom transfer radical polymerization[J]. BIOCHEMICAL ENGINEERING JOURNAL,2016,113(SEP):19-29.
APA Wang, Hong-Yan,Sun, Yan,Zhang, Su-Ling,Luo, Jian,&Shi, Qing-Hong.(2016).Fabrication of high-capacity cation-exchangers for protein chromatography by atom transfer radical polymerization.BIOCHEMICAL ENGINEERING JOURNAL,113(SEP),19-29.
MLA Wang, Hong-Yan,et al."Fabrication of high-capacity cation-exchangers for protein chromatography by atom transfer radical polymerization".BIOCHEMICAL ENGINEERING JOURNAL 113.SEP(2016):19-29.
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