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Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus
Chen, Mei1; Huang, Xuenian1,2; Zhong, Chengwei1,2; Li, Jianjun1,3; Lu, Xuefeng1
2016-09-01
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
Volume100Issue:17Pages:7541-7548
Abstract

Itaconic acid, one of the most promising and flexible bio-based chemicals, is mainly produced by Aspergillus terreus. Previous studies to improve itaconic acid production in A. terreus through metabolic engineering were mainly focused on its biosynthesis pathway, while the itaconic acid-degrading pathway has largely been ignored. In this study, we used transcriptomic, proteomic, bioinformatic, and in vitro enzymatic analyses to identify three key enzymes, itaconyl-CoA transferase (IctA), itaconyl-CoA hydratase (IchA), and citramalyl-CoA lyase (CclA), that are involved in the catabolic pathway of itaconic acid in A. terreus. In the itaconic acid catabolic pathway in A. terreus, itaconic acid is first converted by IctA into itaconyl-CoA with succinyl-CoA as the CoA donor, and then itaconyl-CoA is hydrated into citramalyl-CoA by IchA. Finally, citramalyl-CoA is cleaved into acetyl-CoA and pyruvate by CclA. Moreover, IctA can also catalyze the reaction between citramalyl-CoA and succinate to generate succinyl-CoA and citramalate. These results, for the first time, identify the three key enzymes, IctA, IchA, and CclA, involved in the itaconic acid degrading pathway in itaconic acid producing A. terreus. The results will facilitate the improvement of itaconic acid production by metabolically engineering the catabolic pathway of itaconic acid in A. terreus.

KeywordItaconic Acid Aspergillus Terreus Degradation Pathway Metabolic Engineering
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00253-016-7554-0
Indexed BySCI
Language英语
WOS KeywordISOCITRATE LYASE ; LIVER-MITOCHONDRIA ; METABOLISM ; PSEUDOMONAS ; EXPRESSION ; BIOSYNTHESIS ; ENZYMES ; PH
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
Funding OrganizationNational High Technology Research and Development Program of China(2015AA021003) ; National Natural Sciences Foundation of China(31500042 ; Key Research Program of the Chinese Academy of Sciences(KSZD-EW-Z-016-2) ; Science and Technology Service Network Initiative of the Chinese Academy of Sciences(KFJ-EW-STS077-RW10) ; 31400080)
WOS IDWOS:000381583400016
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ipe.ac.cn/handle/122111/21438
Collection生化工程国家重点实验室
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
Recommended Citation
GB/T 7714
Chen, Mei,Huang, Xuenian,Zhong, Chengwei,et al. Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016,100(17):7541-7548.
APA Chen, Mei,Huang, Xuenian,Zhong, Chengwei,Li, Jianjun,&Lu, Xuefeng.(2016).Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,100(17),7541-7548.
MLA Chen, Mei,et al."Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 100.17(2016):7541-7548.
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