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Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus
Chen, Mei1; Huang, Xuenian1,2; Zhong, Chengwei1,2; Li, Jianjun1,3; Lu, Xuefeng1
2016-09-01
发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN0175-7598
卷号100期号:17页码:7541-7548
摘要

Itaconic acid, one of the most promising and flexible bio-based chemicals, is mainly produced by Aspergillus terreus. Previous studies to improve itaconic acid production in A. terreus through metabolic engineering were mainly focused on its biosynthesis pathway, while the itaconic acid-degrading pathway has largely been ignored. In this study, we used transcriptomic, proteomic, bioinformatic, and in vitro enzymatic analyses to identify three key enzymes, itaconyl-CoA transferase (IctA), itaconyl-CoA hydratase (IchA), and citramalyl-CoA lyase (CclA), that are involved in the catabolic pathway of itaconic acid in A. terreus. In the itaconic acid catabolic pathway in A. terreus, itaconic acid is first converted by IctA into itaconyl-CoA with succinyl-CoA as the CoA donor, and then itaconyl-CoA is hydrated into citramalyl-CoA by IchA. Finally, citramalyl-CoA is cleaved into acetyl-CoA and pyruvate by CclA. Moreover, IctA can also catalyze the reaction between citramalyl-CoA and succinate to generate succinyl-CoA and citramalate. These results, for the first time, identify the three key enzymes, IctA, IchA, and CclA, involved in the itaconic acid degrading pathway in itaconic acid producing A. terreus. The results will facilitate the improvement of itaconic acid production by metabolically engineering the catabolic pathway of itaconic acid in A. terreus.

关键词Itaconic Acid Aspergillus Terreus Degradation Pathway Metabolic Engineering
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00253-016-7554-0
收录类别SCI
语种英语
关键词[WOS]ISOCITRATE LYASE ; LIVER-MITOCHONDRIA ; METABOLISM ; PSEUDOMONAS ; EXPRESSION ; BIOSYNTHESIS ; ENZYMES ; PH
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
项目资助者National High Technology Research and Development Program of China(2015AA021003) ; National Natural Sciences Foundation of China(31500042 ; Key Research Program of the Chinese Academy of Sciences(KSZD-EW-Z-016-2) ; Science and Technology Service Network Initiative of the Chinese Academy of Sciences(KFJ-EW-STS077-RW10) ; 31400080)
WOS记录号WOS:000381583400016
引用统计
文献类型期刊论文
条目标识符http://ir.ipe.ac.cn/handle/122111/21438
专题生化工程国家重点实验室
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
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Chen, Mei,Huang, Xuenian,Zhong, Chengwei,et al. Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016,100(17):7541-7548.
APA Chen, Mei,Huang, Xuenian,Zhong, Chengwei,Li, Jianjun,&Lu, Xuefeng.(2016).Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,100(17),7541-7548.
MLA Chen, Mei,et al."Identification of an itaconic acid degrading pathway in itaconic acid producing Aspergillus terreus".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 100.17(2016):7541-7548.
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